针刺印堂百会风池肾俞穴组对慢性应激模型大鼠海马脑源性神经营养因子基因表达的影响

发布时间：2017-12-27 17:08

本文关键词：针刺印堂百会风池肾俞穴组对慢性应激模型大鼠海马脑源性神经营养因子基因表达的影响　出处：《中医杂志》2017年21期 　论文类型：期刊论文

【摘要】：目的探讨针刺印堂百会风池肾俞穴组治疗抑郁症的可能作用机制。方法 40只SD大鼠随机分为正常组、模型组、针刺组、西药组,每组10只。正常组大鼠正常饲养,其余各组大鼠孤养并进行28天的不可预见的慢性应激刺激建立慢性应激性大鼠模型。造模同时模型组每日予2 ml生理盐水灌胃;针刺组选用印堂、百会、风池(双侧)、肾俞(双侧)进行针刺治疗,每次30 min,每日1次;西药组给予氟西汀胶囊0.18 mg/(kg·d)灌胃,每日1次,各组均灌胃28天;正常组正常饲养不灌胃。检测各组大鼠海马脑源性神经营养因子(BDNF)、环磷腺苷效应元件结合蛋白(CREB)mRNA含量及海马BDNF I-17位点、BDNF I-17 Cp G6位点基因甲基化水平。结果与正常组比较,模型组大鼠海马CREB、BDNF mRNA含量水平明显降低(P0.05或P0.01);针刺组和西药组较模型组大鼠海马CREB、BDNF mRNA含量增加(P0.05)。各组大鼠BDNF I-17位点甲基化水平未出现明显差异(P0.05),在BDNF I-17 Cp G6位点上模型组大鼠甲基化水平明显低于正常组(P0.05),其他各组未出现明显差异。结论针刺印堂百会风池肾俞穴组可有效增加慢性应激模型大鼠海马内CREB、BDNF mRNA水平,可能是其抗抑郁机制之一。
[Abstract]:Objective to investigate the possible mechanism of acupuncture Baihui Fengchi Yintang Shenshu group in the treatment of depression. Methods 40 SD rats were randomly divided into the normal group, the model group, the acupuncture group and the western medicine group, with 10 rats in each group. The rats in the normal group were raised normally, and the rest of the rats were isolated and used for 28 days of unforeseeable chronic stress stimulation to establish a chronic stress rat model. Rats in model group treated 2 ml normal saline; acupuncture group selected Baihui, Yintang, Fengchi (bilateral), Shenshu (bilateral) were treated by acupuncture, 30 min each time, 1 times a day; the western medicine group was given fluoxetine capsules 0.18 mg/ (kg - D) orally, 1 times a day, groups were given the stomach for 28 days; the normal group normal feeding without gavage. Hippocampal brain-derived neurotrophic factor (BDNF), cAMP content binding protein (CREB) mRNA content, and BDNF I-17 and BDNF I-17 Cp G6 gene methylation levels were detected in each group. Results compared with the normal group, the levels of CREB and BDNF mRNA in hippocampus of model group were significantly decreased (P0.05 or P0.01). The content of CREB and BDNF mRNA increased in hippocampus of acupuncture group and Western medicine group (P0.05). There was no significant difference in the methylation level of BDNF I-17 sites in each group (P0.05). The methylation level of the rats in the model group was significantly lower than that in the normal group (P0.05) at the BDNF I-17 Cp G6 site, but no significant difference was found in the other groups. Conclusion acupuncture Baihui Fengchi Yintang Shenshu group can effectively increase the hippocampus of chronic stress rats in CREB, BDNF mRNA level, which may be one of the mechanisms of anti depression.
【作者单位】：中日友好医院;
【基金】：国家自然科学基金(81202766)
【分类号】：R245;R-332
【正文快照】： 抑郁症是精神科常见病、多发病,其发病以显著而持久的心境低落为主要症状,属于心境障碍的一种[1],抑郁症的发病受遗传影响,在环境与基因的交互作用下发病[2]。中医学针刺疗法治疗抑郁症不仅可有效缓解抑郁症状,而且跟抗抑郁药比较,具有起效迅速、无毒副作用等特点,成为抗抑郁

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