大黄素、茶多酚和鱼腥草素钠对TNBS诱导大鼠结肠炎的治疗作用

发布时间：2018-01-01 09:31

  本文关键词：大黄素、茶多酚和鱼腥草素钠对TNBS诱导大鼠结肠炎的治疗作用　出处：《湖南师范大学》2016年硕士论文　论文类型：学位论文

  更多相关文章： 大黄素 茶多酚 TNBS 炎症性肠病 鱼腥草素钠

【摘要】：目的:观察大黄素、茶多酚和鱼腥草素钠对2,4,6-三硝基苯磺酸(TNBS)诱导大鼠结肠炎的治疗作用,并探讨其可能的免疫机制。方法:72只SPF级S-D大鼠随机分为九组,为空白对照组(A组)、大黄素高剂量组(B组,50mg/kg)、大黄素低剂量组(C组,25mg/kg)、茶多酚高剂量组(D组,340mg/kg)、茶多酚低剂量组(E组,170mg/kg)、鱼腥草素钠高剂量组(F组,100mg/kg)、鱼腥草素钠低剂量组(G组,50mg/kg)、柳氮磺吡啶组(H组,100mg/kg)和模型组(I组)。造模当日,B-I组大鼠均以75mg/kgTNBS乙醇溶液灌肠,A组以同体积30%乙醇溶液灌肠。造模24h后,B-H组定时以给定剂量的药物灌胃,A组和I组以相同体积的蒸馏水灌胃。每天1次,持续14d。观察大鼠每日体重变化、大便状态,解剖后观察结肠组织大体改变和HE染色后病理变化并评分,检测血清TNF-α、IL-lβ、IL-4、IL-10水平及ALT、AST和肌酐含量。结果:⑴与A组相比,B-H组和I组大鼠一般情况较差,体重明显减轻,大体评分和组织病理评分均较低,TNF-α和IL-lβ水平明显增高,IL-4和IL-10的表达显著下降,差异有统计学意义(P0.05),但G组与I组相比,大部分指标P0.05,差异无统计学意义。⑵与I组相比,B-H组一般情况较好,体重有所回升,大体状况和组织病理显示炎症程度明显减轻,且TNF-α和IL-lβ表达显著下降,IL-4和IL-10的水平有明显提升,差异有统计学意义(P0.05)。⑶B-H组之间两两比较,B-F组所有指标和H组相比以及B、C组两组相比有差异均无统计学意义(P0.05);然而D、E两组和F、G两组分别比较P值均小于0.05,差异有统计学意义。⑷药物肝肾毒性检测,B-I组大鼠ALT、AST值明显高于A组,B-H组ALT、AST值明显低于I组(P0.05);但A-I组肌酐值无显著性差异(P0.05)。结论:⑴采用75mg/kg浓度的TNBS溶液诱导,成功建立了大鼠结肠炎模型。⑵25mg/kg、50mg/kg剂量的大黄素,170mg/kg、340mg/kg的茶多酚和100mg/kg的鱼腥草素钠灌胃可减轻大鼠结肠病理组织炎症和损伤,降低血清促炎因子TNF-α和IL-lβ水平,升高抑炎因子IL-4和IL-10水平,对大鼠结肠炎有治疗作用,且未见肝肾毒性。⑶170mg/kg、340mg/kg剂量的茶多酚对大鼠结肠炎的治疗作用存在量-效关系;而大黄素在50mg/kg、25mg/kg两种剂量灌胃的情况下,对大鼠结肠炎治疗作用无明显差异。
[Abstract]:Objective: to observe the therapeutic effect of emodin, tea polyphenol and houttuynin sodium on rat colitis induced by TNBS. Methods Seventy-two SPF S-D rats were randomly divided into nine groups: blank control group A group and emodin high dose group B group (50 mg / kg). The emodin concentration was 25 mg / kg in C group, 340 mg / kg in D group and 170 mg / kg in E group (P < 0.05). High dose of houttuyfonate sodium group (100 mg / kg), low dose group (50 mg / kg) of sodium houttuynate sodium and sulfasalopyridine group (H group). On the same day, the rats in the B-I group were enema with 75 mg / kg TNBS ethanol solution. The rats in group A were perfused with the same volume of 30% ethanol solution 24 hours later, the rats in group B and H were perfused with the same volume of distilled water once a day. For 14 days, the changes of body weight, stool status, colonic gross changes and pathological changes after HE staining were observed, and the serum TNF- 伪 IL-l 尾 and IL-4 were detected. Results compared with group A, IL-10 level and alt AST and creatinine content in group 鈪，

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