异常黑胆质成熟剂对病证结合痴呆大鼠模型海马组织线粒体酶活性的影响

发布时间：2018-01-01 10:19

本文关键词：异常黑胆质成熟剂对病证结合痴呆大鼠模型海马组织线粒体酶活性的影响　出处：《中华中医药杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:探讨异常黑胆质成熟剂对病证结合痴呆大鼠模型海马组织线粒体酶活性的影响。方法:根据传统维吾尔医学理论,应用多因素复合作用来建立异常黑胆质证载体动物模型之后,将凝集态β淀粉样蛋白1-40(Aβ1-40)定位注射到右侧海马,进一步建立异常黑胆质型痴呆病证结合模型,SD大鼠96只随机分为正常对照组、单纯痴呆组、异常黑胆质证模型组、异常黑胆质型AD组、异常黑胆质成熟剂干预组(分为高、中、低剂量组)、阳性对照组,分离海马以生化法检测Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase、SDH的活性。结果:与正常对照组比较,单纯痴呆组Na~+-K~+-ATPase酶活性及SDH活性显著降低(P0.05),异常黑胆质型AD组Na~+-K~+-ATPase酶活性显著升高(P0.01)、Ca~(2+)-Mg~(2+)-ATPase酶活性及SDH活性显著降低(P0.05,P0.01),异常黑胆质证模型组Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase酶活性显著升高(P0.01,P0.05)、SDH活性显著降低(P0.05),异常黑胆质成熟剂各剂量干预组Ca~(2+)-Mg~(2+)-ATPase酶活性上升(P0.05)。与异常黑胆质型AD组比较,异常黑胆质成熟剂中、高剂量干预组Na~+-K~+-ATPase酶活性及SDH活性显著上升(P0.05)。与单纯痴呆组比较,异常黑胆质证模型组及阳性对照组Na~+-K~+-ATPase酶活性显著升高(P0.05),异常黑胆质型AD组Ca~(2+)-Mg~(2+)-ATPase酶活性及SDH活性降低(P0.05)。结论:异常黑胆质成熟剂可能通过改善病症结合痴呆模型大鼠脑组织能量的合成和分解、调节线粒体功能而起作用。
[Abstract]:Objective: to investigate the effect of abnormal black bile maturation on mitochondrial enzyme activity in hippocampus of rats with disease syndrome combined with dementia. Methods: according to the traditional Uygur medical theory. After the animal model of abnormal black bile syndrome carrier was established by multifactor compound action, the agglutinated 尾 -amyloid 1-40A 尾 1-40) was injected into the right hippocampus. Further more, 96 SD rats were randomly divided into normal control group, simple dementia group, abnormal black gallbladder syndrome model group and abnormal black gallbladder type AD group. The abnormal black bile maturation agent intervention group was divided into high, middle and low dose groups and positive control group. The hippocampus was isolated and detected by biochemical method for Na ~ -K ~ -ATPase. Results: compared with the normal control group, the activity of Ca~(2 was compared with that of the normal control group. The activity of Na ~ -K ~ -ATPase and SDH in simple dementia group decreased significantly (P 0.05). The activity of Na ~ -K ~ -ATPase increased significantly in AD group (P 0.01). The activity of Ca~(2 and SDH decreased significantly (P 0.05 P 0.01). The enzyme activity of Na ~ -K ~ -AT Paseate CaO2-Mg-Mg-PS-ATPase was significantly increased in the model group of abnormal black gallbladder syndrome (P0.01 ~ + P0.05). The activity of SDH decreased significantly (P 0.05). The activity of Ca~(2 mg-mg 2 + -ATPase in the abnormally black bile maturation group was higher than that in the abnormal dark-gallbladder type AD group (P 0.05), and compared with that in the abnormal black bile type AD group (P < 0.05). The activity of Na ~ -K ~ -ATPase and SDH in the high dose intervention group was significantly higher than that in the simple dementia group. The activity of Na ~ -K ~ -ATPase was significantly increased in the model group of abnormal black gallbladder syndrome and the positive control group (P0.05). The activity of Ca~(2 and SDH decreased in AD group with abnormal black bile type (P0.05). Conclusion: abnormal black bilirubin maturation agent may improve the synthesis and decomposition of brain energy in dementia model rats. Regulate the function of mitochondria and play a role.
【作者单位】：新疆医科大学附属中医医院肿瘤科;新疆医科大学维吾尔医学院;
【基金】：国家自然科学基金项目(No.81202773)~~
【分类号】：R29
【正文快照】： 乌鲁木齐830011)阿尔茨海默病(Alzheimer’s disease,AD)是由多种原因引起的神经系统退行性疾病,病程缓慢且不可逆,主要以认知和记忆功能损害及减退、精神行为症状以及日常生活能力减退为特征[1]。截至2010年,全世界范围内AD患者已达到3 560万[2]。研究人员发现,预计到2050年

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