电针刺激天枢穴对急性重症胰腺炎大鼠肺组织AQP-1表达的影响

发布时间：2018-01-02 01:10

本文关键词：电针刺激天枢穴对急性重症胰腺炎大鼠肺组织AQP-1表达的影响　出处：《山东医药》2017年22期 　论文类型：期刊论文

【摘要】：目的观察电针刺激天枢穴对急性重症胰腺炎大鼠肺组织AQP-1表达的影响。方法将60只雄性SD大鼠随机分为正常对照组、正常电针组、模型组、电针模型组,各15只。正常对照组、正常电针组不做任何处理;模型组、电针模型组逆行胰胆管注射5%牛磺胆酸钠制备急性重症胰腺炎模型。正常电针组、电针模型组在模型制备成功2 h后,电针刺激大鼠天枢穴。造模8 h后,采用RT-PCR和免疫组化法分别检测各组大鼠肺组织中AQP-1mRNA、蛋白。结果模型组大鼠肺组织AQP-1 mRNA、蛋白表达低于正常对照组和正常电针组(P均0.05),电针模型组大鼠肺组织AQP-1 mRNA、蛋白表达高于模型组(P均0.05),正常对照组与正常电针组大鼠肺组织AQP-1 mRNA、蛋白表达比较差异无统计学意义。结论电针刺激天枢穴可升高急性重症胰腺炎大鼠肺组织AQP-1的表达。
[Abstract]:Objective to observe the effect of electroacupuncture on the expression of AQP-1 in lung tissue of rats with severe acute pancreatitis. Methods 60 male SD rats were randomly divided into normal control group, normal electroacupuncture group and model group. Electroacupuncture model group (n = 15), normal control group (n = 15) and normal electroacupuncture group (n = 15) were not treated. Model group, electroacupuncture model group, retrograde injection of 5% sodium taurocholate into the pancreatic duct to make acute severe pancreatitis model, normal electroacupuncture group, electroacupuncture model group 2 hours after the establishment of the model. The rats were stimulated by electroacupuncture at Tianshu point. After 8 hours of modeling, the AQP-1mRNA in lung tissue of each group was detected by RT-PCR and immunohistochemistry respectively. Results the expression of AQP-1 mRNAs in lung tissue of model group was lower than that of normal control group and normal electroacupuncture group (P 0.05). The expression of AQP-1 mRNAs in the lung tissue of the model group was higher than that of the model group (P 0.05), and the AQP-1 mRNA in the lung tissue of the normal control group and the normal electroacupuncture group was higher than that of the control group and the normal electroacupuncture group. Conclusion stimulation of Tianshu point by electroacupuncture can increase the expression of AQP-1 in lung tissue of rats with severe acute pancreatitis.
【作者单位】：中国人民解放军第181中心医院;桂林医学院附属医院;广东省中医院;
【基金】：广西高校科学技术研究项目(YB2014273)
【分类号】：R245
【正文快照】： 3广东省中医院)急性重症胰腺炎(SAP)病情凶险,且常并发急性肺损伤(ALI)加重病情。ALI主要病理特征为肺血管内皮细胞及肺泡上皮细胞广泛性损伤,全身炎症反应综合征在肺部表现为肺部弥漫性渗出和难以用单纯吸氧纠正的低氧血症[1]。此种胰源性肺损伤的终末结局为呼吸窘迫综合征,

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