额尔敦—乌日勒对动脉粥样硬化易损斑块家兔血管内皮功能的影响

发布时间：2018-01-02 01:28

本文关键词：额尔敦—乌日勒对动脉粥样硬化易损斑块家兔血管内皮功能的影响　出处：《内蒙古医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的通过观测额尔敦-乌日勒对家兔动脉粥样硬化易损斑块模型血管内皮因子ET、PAF、TXA-2、PGI2、VEGF、E-selectin、SDF-1以及血管内皮凝血纤溶物质t-PA、PAI-1、Fbg的影响,明确其稳定斑块的作用机制。方法将50只家兔,随机分为正常组和造模组。正常组10只不进行造模,普通饲料喂养;造模组40只采用高脂饮食喂养、免疫损伤、球囊拉伤术复合因素造模,建立家兔动脉粥样硬化易损斑块模型。8周末造模成功后,将造模组随机分为模型组、辛伐他汀组、额尔敦-乌日勒组,正常组和模型组分别灌服同体积的蒸馏水,给药容量均为4ml·kg-1·d-1;辛伐他汀组给予辛伐他汀5mg·kg-1·d-1;额尔敦-乌日勒组给予额尔敦-乌日勒0.4g·kg-1·d-1,连续给药16周。第24周末实验结束,将最终完成实验的39只家兔(正常组10只、模型组10只、额尔敦-乌日勒组10只、辛伐他汀组9只)处死取材。观测以下指标:1.运用免疫组化法检测各组家兔主动脉血管内皮因子ET、PAF、VEGF、SDF-1、t-PA、PAI的表达情况。2.运用Elisa方法检测各组家兔血清中血管内皮因子ET、PAF、TXA-2、PGI2、VEGF、E-selectin、SDF-1、t-PA、PAI、Fbg的浓度。结果1.免疫组化结果显示:(1)额尔敦-乌日勒能降低主动脉ET、PAF表达(P0.01),在降低ET表达上优于辛伐他汀(P0.05)。(2)与模型组相比,额尔敦-乌日勒组主动脉VEGF和SDF-1的表达显著减少(P0.05),但对E-selectin的影响无显著性差异(P0.05)。(3)与模型组比较,额尔敦-乌日勒组主动脉PAI-1的表达显著降低(P0.01),且表达量低于辛伐他汀组(P0.05),但对t-PA的影响无统计学差异(P0.05)。2.Elisa检测结果显示:(1)与模型组比较,额尔敦-乌日勒显著降低血清ET、PAF(P0.01),在降低ET水平上优于辛伐他汀组。(2)额尔敦-乌日勒能显著降低血清TXA-2的水平、升高PGI2的水平(P0.05)。(3)额尔敦-乌日勒能显著降低家兔血清VEGF和SDF-1的浓度(P0.01),且对其降低SDF-1的浓度优于辛伐他汀组(P0.05)。(4)与模型组比较,额尔敦-乌日勒组家兔血清PAI-1及Fbg水平显著降低(P0.01),但对t-PA的影响无统计学差异(P0.05);结论额尔敦-乌日勒具有改善动脉粥样硬化血管内皮功能的作用,其可能通过降低血管内皮因子ET、PAF、TXA-2、VEGF、SDF-1的水平以及升高血清PGI2的水平;同时也可通过降低血清血管内皮凝血纤溶物质PAI-1、Fbg水平来发挥抗动脉粥样硬化和稳定易损斑块的作用。
[Abstract]:Objective to observe the effect of Erden-Uzhler on vascular endothelial factor (ETF) TXA-2 PGI2 (VEGFN) E-selectin in atherosclerotic vulnerable plaque model in rabbits. The effects of SDF-1 and the fibrinolytic substance t-PAI-1Fbg on the stability of plaque were determined. Methods 50 rabbits were selected. They were randomly divided into normal group and model group. The model group (40 rats) were fed with high fat diet, immune injury and balloon wound were used to make the model. The model group was randomly divided into model group after the successful establishment of atherosclerotic plaque model at the end of week .8. Simvastatin group, Erden-Uril group, normal group and model group were given distilled water of the same volume, respectively, and the dosage was 4ml 路kg-1 路d-1; Simvastatin group was given simvastatin 5 mg 路kg-1 路d -1; Erden-Uzhler group was given 0.4 g 路kg-1 路d-1 of Erden-Ujil for 16 weeks. At the end of the 24th week, 39 rabbits (10 normal rabbits) were selected to complete the experiment at the end of the 24th week. Ten rats in the model group, 10 in the Erden-Ure group and 9 in the simvastatin group were killed. The following indexes were observed: 1. The endothelium factor ETF-PAF in aorta of rabbits in each group was detected by immunohistochemical method. The expression of VEGFF-1t-PAI in rabbit serum was detected by Elisa method. Results 1.Immunohistochemical results showed that Erden-1) Erden-Uzhler could decrease the et of aorta. The expression of PAF was higher than that of simvastatin (P0.05, P0.05, P < 0.05), which was better than that of the model group in decreasing the expression of et. The expression of VEGF and SDF-1 in the aorta of Erden-Ujil group was significantly decreased (P 0.05). However, there was no significant difference in the effect of E-selectin on the expression of PAI-1 in the aorta of the Erden-Ujil group (P 0.05) compared with the model group (P 0.01). The expression of t-PA in simvastatin group was lower than that in simvastatin group, but there was no significant difference in the effect of t-PA. Erden-Uzhler significantly reduced the level of serum ETF-PAFN P0.01, which was superior to that of simvastatin group in lowering the level of TXA-2.) Erden-Uriel could significantly reduce the level of serum TXA-2. Raising the level of PGI2 (P0.05N.) Erden-Uzhler could significantly decrease the concentration of VEGF and SDF-1 in serum of rabbits (P 0.01). The decrease of SDF-1 concentration was better than that of simvastatin group (P0.05A. 4) and compared with the model group. The levels of serum PAI-1 and Fbg in Erden-Uzhler group decreased significantly (P 0.01), but there was no significant difference in the effect of t-PA on serum PAI-1 and Fbg. Conclusion Erden-Uzhler can improve the endothelial function of atherosclerotic vessels, which may be through the reduction of vascular endothelial factor ETF-PAFTXA-2VEGF.Conclusion Erden-Uzhler can improve the endothelial function of atherosclerotic vessels. The level of SDF-1 and the level of serum PGI2; At the same time, it can also play the role of anti-atherosclerosis and stabilization of vulnerable plaque by reducing the level of PAI-1 FBG.
【学位授予单位】：内蒙古医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R29

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