广西民族药材走马胎、血党质量标准实验研究

发布时间：2018-01-03 02:27

本文关键词：广西民族药材走马胎、血党质量标准实验研究　出处：《广西中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:通过实验研究广西民族药材走马胎、血党的质量标准,初步拟定走马胎和血党的质量标准及建立走马胎、血党的HPLC指纹图谱研究方法,为药材的质量控制提供可靠的依据。方法:(1)参照《中国药典》2015年版通则对走马胎和血党药材进行水分、总灰分、浸出物的测定。(2)运用微波消解ICP-MS检测走马胎和血党药材中重金属Cu、As、Hg、Cd、Pb的含量。(3)以没食子酸为对照品,参照药典的方法进行TLC鉴别。(4)运用HPLC,测定广西不同产地和采集时间的走马胎和血党药材中没食子酸的含量。(5)运用HPLC,通过相似度软件对不同产地的药材进行分析,得到指纹图谱的共有模式。结果:(1)走马胎药材中水分含量5.65%~12.77%;总灰分含量1.54%~2.63%;酸不溶性灰分含量0.07%~0.62%;浸出物含量10.14%~17.36%。血党药材水分含量10.69%~12.50%;总灰分含量4.07%~4.85%;酸不溶性灰分含量0.32%~1.05%;浸出物含量12.03%~17.94%。(2)走马胎药材中重金属Cu、As、Hg、Pb的含量均符合标准规定;Cd的含量除2013年7月广西来宾市金秀县采集的走马胎药材外,其余批次均符合规定。10批血党药材中重金属Cu、Cd、Hg均符合药典标准规定,其中有2批血党药材中As的含量超过标准规定,4批血党药材中Pb的含量不符合标准。(3)走马胎和血党药材的TLC鉴别方法可靠且重复性好。得到清晰的斑点,分离效果好。(4)HPLC法测定没食子酸的含量,经方法学验证,具有良好的线性关系、精密度、重复性和稳定性,加样回收率均在95%-105%范围内。(5)不同批次的走马胎和血党药材指纹图谱相似度结果均大于0.9,各特征峰分离度较好,符合指纹图谱检测要求。结论:综合上述的实验结果,初步建立了走马胎和血党药材的质量标准,为走马胎和血党药材的评价和分析提供了较科学的实验依据。初步建立走马胎和血党药材HPLC指纹图谱的研究方法,该方法具有准确性、可靠性、特征性强的特点,稳定性、精密度和重复性结果均符合要求,可广泛运用于走马胎和血党药材的质量评价。
[Abstract]:Objective: to study the quality standard of horse fetus and blood party of Guangxi nationality medicinal materials, to draw up the quality standard of walking horse fetus and blood party and to establish the HPLC fingerprint study method of walking horse fetus and blood party. Methods according to the general rules of the 2015 edition of Chinese Pharmacopoeia, the moisture content and total ash content of the Chinese medicinal materials were determined. Determination of the extract by microwave digestion ICP-MS method for the determination of the content of heavy metal CuAsAsHgfen CDPb. 3) Gallic acid was used as the reference substance. According to the method of Pharmacopoeia, TLC was used to identify the content of Gallic acid in different producing areas and collecting time in Guangxi. The content of Gallic acid was determined by HPLC. The common pattern of fingerprint was obtained by using similarity software. Results: the water content in the crude drug was 5.65 ~ 12.77% and the content of water was 5.65 ~ (12.77). The total ash content was 1.54 and 2.63. The content of acid insoluble ash was 0.07% and 0.62%; The content of the extract was 10.14% and 17.36%. The water content of Xue Dang medicinal material was 10.699.12.50%. The total ash content is 4.07 and 4.85; The acid insoluble ash content was 0.32% and 1.05%; The content of the heavy metal Cu _ (AsA) H _ (+) H _ (G) Pb in the extract 12.03 ~ 17.94 ~ (2) is in accordance with the standard. In addition to the contents of CD collected in July 2013 in Jinxiu County of Laibin City, Guangxi, the other batches were in accordance with the requirements of the Pharmacopoeia standard for the heavy metal Cu ~ (2 +) CD ~ (2 +) Hg in 10 batches of Xue Dang medicinal materials. Among them, 2 batches of as in Xue Dang medicinal materials exceeded the standard. The content of Pb in 4 batches of Xue Dang medicinal materials was not up to the standard. 3) the TLC identification method of the Hedyotis and Xue Dang herbs was reliable and reproducible, and the clear spots were obtained. The content of Gallic acid was determined by HPLC. The method was proved to be linear, precise, reproducible and stable. The recoveries were in the range of 95 ~ 105%.) the similarity between the fingerprint of different batches of walking horse fetus and Xue Dang was greater than 0.9, and the separation degree of each characteristic peak was better. Conclusion: based on the above experimental results, the quality standard of the crude drugs was established preliminarily. It provides a scientific experimental basis for the evaluation and analysis of walking horse fetus and Xueduan medicinal materials. A preliminary study method of HPLC fingerprint of walking horse fetus and Xue Dou medicinal materials is established. The method is accurate and reliable. The characteristic characteristics, stability, precision and reproducibility all meet the requirements, and can be widely used to evaluate the quality of the foetus and Xue Dang medicinal materials.
【学位授予单位】：广西中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R29

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