针灸对DDP化疗小鼠肝脏组织中MDA和CAT含量的影响

发布时间：2018-01-08 12:26

本文关键词：针灸对DDP化疗小鼠肝脏组织中MDA和CAT含量的影响　出处：《时珍国医国药》2017年10期 　论文类型：期刊论文

【摘要】：目的通过观察针灸对顺铂(DDP)化疗小鼠肝脏组织中丙二醛(MDA)和过氧化氢酶(CAT)表达含量的影响,阐释针灸对顺铂化疗所致小鼠肝脏损伤的保护机制。方法选用清洁级,雄性昆明种小鼠40只,体重(20±2)g,随机分为空白组、模型组、针刺组、艾灸组,每组10只。治疗前1天,除空白组外,其余3组小鼠按体重腹腔注射顺铂0.01ml/g(10mg/kg),空白组注射同等剂量的0.9%Nacl溶液,24h后模型成功。针刺组和艾灸组分别给予针刺、艾灸治疗,均选取"大椎""肝俞""肾俞""足三里(后三里)",每天1次,空白组和模型组每日同时间陪同固定不治疗,连续5天。禁食1天后取材,用ELISA法检测各组小鼠肝脏组织中MDA和CAT的含量。结果治疗5天后,与空白组比,模型组、针刺组和艾灸组小鼠肝脏组织中MDA含量升高,CAT含量降低,均有显著性差异(P0.05);与模型组比,针刺组、艾灸组小鼠肝脏组织中MDA含量降低,CAT含量升高,均有显著性差异(P0.05)。结论针刺和艾灸可以明显抑制DDP模型小鼠肝脏组织中MDA的表达,促进CAT的表达,减轻因DDP化疗所致的肝脏损伤,起到增效减毒的作用,应该是针灸减轻DDP化疗后肝脏损伤的作用机制之一。
[Abstract]:Objective to observe the effect of acupuncture and moxibustion on the expression of malondialdehyde (MDA) and catalase (catalase) in liver of cisplatin / DDP chemotherapy mice. To explain the protective mechanism of acupuncture and moxibustion on liver injury induced by cisplatin chemotherapy in mice. Methods 40 Kunming mice of clean grade, weight of 20 卤2g were randomly divided into blank group, model group and acupuncture group. The mice in the moxibustion group were injected intraperitoneally with cisplatin 0.01ml / g / g 10 mg / kg one day before treatment except the blank group. The model of the blank group was successfully injected with the same dose of 0.9Nacl solution 24 hours later. The acupuncture group and the moxibustion group were treated with acupuncture and moxibustion respectively. "Dazhui", "Ganshu", "Shenshu", "Zusanli", once a day, blank group and model group were fixed at the same time every day for 5 consecutive days. The contents of MDA and CAT in liver tissue of each group were detected by ELISA method. Results after 5 days of treatment, compared with the blank group, the content of MDA in the liver of the model group, acupuncture group and moxibustion group was higher than that of the control group. The content of CAT decreased with significant difference (P 0.05). Compared with the model group, the content of MDA in the liver of the acupuncture group and moxibustion group decreased and the content of cat increased. Conclusion Acupuncture and moxibustion can obviously inhibit the expression of MDA and promote the expression of CAT in liver of DDP model mice. It is suggested that the mechanism of acupuncture and moxibustion to reduce liver injury after DDP chemotherapy is to alleviate the liver injury caused by DDP chemotherapy.
【作者单位】：河南中医药大学针灸推拿学院;河南中医药大学国际教育学院;河南中医药大学第一附属医院;
【基金】：国家自然科学基金重大计划项目(No.81072884) 河南省中医药科学研究专项课题(No.2016ZY2072) 河南中医药大学博士基金课题(BSJJ2016-08)
【分类号】：R245
【正文快照】： 化疗是目前恶性肿瘤综合治疗的主要手段之一[1]。顺铂(cisplatin,DDP)因具有较强的广谱抗癌作用,仍是当前化疗中最常用的药物之一。然而,顺铂在发挥高效抗癌活性的同时,也对机体产生严重的毒副作用,尤其对消化系统、骨髓、肝、肾及内耳等容易产生毒性损伤。其中顺铂的肝毒性往

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