针刺对脂多糖（LPS）诱导的炎性痛小鼠TNF-α表达的影响

发布时间：2018-01-09 15:30

本文关键词：针刺对脂多糖（LPS）诱导的炎性痛小鼠TNF-α表达的影响　出处：《南京中医药大学》2017年硕士论文　论文类型：学位论文

更多相关文章：针刺 LPS 慢性炎症 TRPV1 TNF-α

【摘要】：目的:探讨针刺对脂多糖(lipopolysaccharide,LPS)诱导的慢性炎症疼痛行为的影响以及对慢性炎症的改善机制。方法:将24只C57BL/6J雄性小鼠(6-8周龄,22±5g)随机分为3组:空白组小鼠腹腔注射4ml.kg-1生理盐水;模型组小鼠腹腔注射脂多糖(LPS)3mg.kg-1,制造慢性炎症小鼠模型;针刺组对于慢性炎症小鼠给予针刺双侧"足三里"穴治疗。对于三组小鼠不同处理后,分别采用Von Frey检测各组小鼠的机械缩足反射阈值(mechanical withdrawal threshold,MWT),采用酶联免疫(euzymelinked immunosorbent assay,ELISA)法检测各组小鼠肿瘤坏死因子α(tumor necrosis factor,TNF-α)含量,采用蛋白质免疫印迹杂交(Western blot)法检测各组小鼠背根神经节(dorsal root ganglion,DRG)神经元与香草酸瞬时受体亚型1(transient receptor potential vanilloid 1,TRPV 1)表达的差异,采用免疫组织化学法检测各组小鼠DRG中TRPV1蛋白阳性细胞数的表达。结果:针刺组与模型组相比较,小鼠机械痛阈明显升高(P0.05);血清TNF-α含量明显降低(P0.05);DRG神经元TRPV1蛋白表达降低(P0.05),DRG神经元TRPV1蛋白阳性细胞数的表达降低(P0.05)。结论:针刺治疗可以减少机体炎症因子的表达,同时也改变DRG神经元中TRPV1的表达,从而缓解和抑制慢性炎症所导致的疼痛。
[Abstract]:Objective: to study the effect of acupuncture on lipopolysaccharide of lipopolysaccharide. Methods: 24 C57BL / 6J male mice were selected for 6-8 weeks of age. 22 卤5g) were randomly divided into three groups: mice in blank group were intraperitoneally injected with 4 ml 路kg ~ (-1) normal saline; The model group mice were injected intraperitoneally with lipopolysaccharide (LPSN) 3 mg 路kg ~ (-1) to produce chronic inflammatory mice model. Acupuncture group for chronic inflammation mice were given acupuncture bilateral "Zusanli" point treatment. For the three groups of mice after different treatment. Von Frey was used to detect the mechanical withdrawal threshold of mechanical reflex in each group. Enzyme linked immunized immunosorbent assay was used. The content of tumor necrosis factor- 伪 (TNF- 伪) in mice was determined by Elisa. The root ganglion of dorsal root ganglion (DRG) was detected by Western blot. Transient receptor potential vanilloid 1 between DRG neurons and vanillic acid transient receptor subtype 1. The expression of TRPV1 protein was detected by immunohistochemical method in DRG of mice in each group. Results: compared with model group, the expression of TRPV1 protein in acupuncture group was compared with that in model group. The mechanical pain threshold increased significantly in mice. The content of serum TNF- 伪 decreased significantly (P 0.05). The expression of TRPV1 protein in DRG neurons was decreased (P 0.05). The expression of TRPV1 protein positive cells in DRG neurons decreased P0.05.Conclusion: acupuncture treatment can reduce the expression of inflammatory factors. It also changes the expression of TRPV1 in DRG neurons, which alleviates and inhibits the pain caused by chronic inflammation.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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