穴位埋线对血管性痴呆模型大鼠学习记忆及海马SOD、MDA的影响

发布时间：2018-01-09 21:59

本文关键词：穴位埋线对血管性痴呆模型大鼠学习记忆及海马SOD、MDA的影响　出处：《黑龙江中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:观察百会、肾俞穴位埋线对血管性痴呆模型大鼠学习记忆、神经细胞及海马SOD、MDA的影响,探索百会、肾俞穴位埋线是否有脑神经保护作用及抗氧化作用,为血管性痴呆治疗提供新的依据。方法:造模前行水迷宫实验排除特异性大鼠,随机假手术组10只,以经改良的 Pulsinelli' s4 血管阻断法(four-vesselocclusion,4-VO)建立 VD 大鼠模型[1]后随机数表法分为VD模型组、穴位埋线组、针刺组。埋线组取穴:肾俞(双)、百会,每隔7天埋线1次,共埋2次;针刺组:治疗之日起每日上午8时到12时之间进行针刺,百会,神庭穴向后斜刺,大椎,足三里,直刺平补平泻手法捻转1分钟,后留针20分钟,针刺每日一次,共针刺16天。模型组:造模成功后除常规饲养每日消毒观察大鼠日常行为外不予任何治疗处理。假手术组:麻醉后,行背侧颈正中切口,不进行椎动脉烧灼,再行腹侧颈正中切口,分离双侧颈总动脉,穿线备用,但不进行颈总动脉夹闭,24小时候缝合切口。治疗期间常规饲养每日消毒观察大鼠日常行为外不予任何治疗处理。以上动物均在造模后、治疗结束后行Morris水迷宫检测各组大鼠学习记忆能力,水迷宫试验后随机抽取各组中大鼠处死取含海马的脑组织检测细胞凋亡情况及SOD,MDA。结果;经水迷宫实验检测VD模型组表现出明显的学习记忆障碍,SOD活性下降,MDA含量上升,与假手术组相比差异有统计学意义(P0.01);埋线组与针刺组两组与模型组相比治疗后SOD活性均有所上升,MDA含量均有所下降,且寻找跳台次数明显增多,潜伏期缩短,但埋线组优于针刺组。各组抽取的大鼠脑切片做HE染色,光学显微镜下辨识海马锥体细胞变化。发现假手术组锥体细胞排行紧凑,整体构架规整,核清晰可见并深染。模型组则呈弥散空泡样变大,核仁缩小。埋线组和针刺组细胞空泡弥散较模型组少,细胞构架较清楚,核仁也可见。结论:百会、肾俞穴位埋线可以改善大鼠的学习记忆能力,提高大鼠大脑SOD活性,降低MDA含量,对大鼠脑组织损伤具有明显的保护作用。
[Abstract]:Objective: to observe the effects of catgut embedding at Baihui and Shenshu points on learning and memory, nerve cells and MDA in hippocampus of rats with vascular dementia. Whether the Shenshu acupoint catgut embedding has the brain nerve protection and the antioxidation function, provides the new basis for the vascular dementia treatment. Methods: the water maze experiment before making the model excludes the specific rats, 10 rats in the random sham-operation group. Establishment of VD rat model by modified four-vessel inclusion 4-VO4 vessel occlusion method. [(1) the later random number table method was divided into VD model group, acupoint embedding group and acupuncture group. The acupoints in the catgut embedding group were collected: Shenshu (Shuangli, Baihui, once every 7 days), buried twice; Acupuncture group: daily acupuncture between 8:00 and 12:00 every day from the day of treatment, Baihui, Shenting point backward oblique prick, Dazhui, Zusanli, straight prick Pingbu Pingxiao technique twirled for 1 minute, then kept needle for 20 minutes. Acupuncture once a day for 16 days. Model group: after the model was successfully made, there was no treatment except routine feeding and daily disinfection to observe the daily behavior of the rats. The sham operation group: after anesthesia, the dorsal cervical median incision was performed. No cauterization of vertebral artery, ventral median cervical incision, separation of bilateral common carotid artery, puncture line reserve, but no common carotid artery occlusion. 24 hours suture incision. During the treatment period, routine feeding, disinfection and observation of the daily behavior of the rats were not treated with any treatment. All the above animals were treated after the establishment of the model. After the treatment, the learning and memory ability of the rats in each group was measured by Morris water maze. After the water maze test, the rats in each group were randomly selected and killed to detect apoptosis and SOD in the hippocampus. MDA. results; In VD model group, the activity of SOD decreased and the content of MDA increased by water labyrinth test. The difference was statistically significant compared with that of sham operation group (P 0.01). Compared with the model group, the activity of SOD in the catgut embedding group and acupuncture group decreased, and the frequency of searching for the platform increased and the incubation period was shortened. But the embedding group was superior to the acupuncture group. The brain slices of each group were stained by HE and the changes of hippocampal pyramidal cells were identified under optical microscope. The results showed that the pyramidal cells in the sham operation group were compact and the whole structure was regular. The nuclei were clearly visible and deeply stained. The model group showed diffuse vacuoles and reduced nucleoli. The number of vacuoles in the embedding group and acupuncture group was less than that in the model group. The cell structure was clear and the nucleolus was also visible in the model group. Conclusion: Baihui can be seen. The catgut embedding at Shenshu acupoint can improve the learning and memory ability of rats, increase the activity of SOD and decrease the content of MDA in the brain of rats. It has obvious protective effect on brain tissue injury in rats.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245;R-332

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