针刺对单眼剥夺大鼠视皮层突触结构可塑性的调节机制研究

发布时间：2018-01-10 01:08

本文关键词：针刺对单眼剥夺大鼠视皮层突触结构可塑性的调节机制研究　出处：《中华中医药杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的:揭示和探讨敏感期内针刺对单眼剥夺(MD)大鼠视皮层结构可塑性的调节作用和机制。方法:将60只SD大鼠随机分为5组,每组12只。其中,空白组不予任何处理;模型组和敏感早、中、末期针刺组采用单侧眼睑缝合的方法复制MD模型后,模型组不予任何治疗,各针刺组分别在造模后第3、12和21天开始针刺治疗。每组治疗结束后进行图形视觉诱发电位(P-VEP)和视皮层17区神经元的形态学检测。结果:与空白组比较,模型组大鼠P_(100)波幅值显著降低(P0.01),潜伏期明显延迟(P0.01);而各针刺组P_(100)波幅值明显升高(P0.01,P0.05),潜伏期明显提前(P0.01,P0.05),较模型组均有显著改善;且敏感早期针刺对P-VEP波形的影响大于敏感中期和敏感晚期针刺。模型组大鼠视皮层17区神经元突触结构出现明显病理损伤,表现为突触结构不清晰,突触间隙消失,髓鞘板层结构松解,细胞间出现局灶状溶解区及髓鞘样改变。而各针刺组大鼠损伤的突触结构均有不同程度修复,其中,早期针刺的修复作用优于中期和晚期针刺。模型组大鼠视皮层17区突触活性区长度明显缩短(P0.01),而各针刺组突触活性区长度均不同程度延长(P0.01)。结论:单眼剥夺后大鼠视皮层17区神经元出现电生理及突触超微结构异常改变,表明敏感期内视皮层存在结构和功能可塑性变化;针刺干预能显著调节视觉功能的可塑性变化,并且疗效与治疗时机密切相关。
[Abstract]:Objective: to explore the effect and mechanism of acupuncture in sensitive period on the plasticity of visual cortex in rats with monocular deprivation of MD.Methods: sixty SD rats were randomly divided into 5 groups, 12 rats in each group. Blank group does not deal with any; After unilateral eyelid suture was used to replicate MD model in model group and early, middle and late stage acupuncture group, no treatment was given in the model group, and each acupuncture group was in the third part of the model. After 12 and 21 days of treatment, the morphologic changes of the neurons in the visual evoked potential (P VEP) and the 17 area of the visual cortex were detected. Results: the results were compared with those in the blank group. In the model group, the amplitude of PX 100) decreased significantly (P 0.01), and the latency was significantly delayed (P 0.01). The amplitudes of P0. 01 and P0. 05 in each acupuncture group were significantly higher than those in the model group, and the latency of P0. 01 and P0. 05 were significantly improved compared with the model group. The effect of early sensitive acupuncture on P-VEP waveform was greater than that on late sensitive acupuncture. In the model group, the synaptic structure of 17 neurons in the visual cortex was obviously damaged, and the synaptic structure was not clear. The synaptic gap disappeared, the lamellar structure of the myelin sheath was loosened, the focal dissolving area and myelin sheath were found in the intercellular area, and the synaptic structures of all the acupuncture groups were repaired to varying degrees. The repair effect of early acupuncture was better than that of middle and late acupuncture. In the model group, the length of synaptic active region in the 17 region of visual cortex was shortened significantly (P 0.01). However, the length of synaptic active area in each acupuncture group was prolonged to some extent (P 0.01). Conclusion: the electrophysiological and synaptic ultrastructural changes of 17 neurons in the visual cortex of the rats after monocular deprivation were abnormal. The results showed that there were structural and functional plasticity changes in the visual cortex during the sensitive period. Acupuncture intervention can significantly regulate the plasticity of visual function, and the therapeutic effect is closely related to the timing of treatment.
【作者单位】：甘肃中医药大学针灸推拿学院;甘肃省中医院针灸科;
【基金】：国家自然科学基金项目(No.81260560,No.81660816)~~
【分类号】：R245
【正文快照】： 弱视是严重影响少年儿童视功能健康发育的疾病之一,有统计显示弱视在我国的发病率为3.0%-3.8%,其中儿童占25.0%[1]。现代研究发现,视皮层形态结构的改变是导致弱视发生的根本原因[2],且弱视发病的时间多为视觉发育的敏感期(即可塑期),因此在敏感期内进行干预治疗,使受损的神经

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