电针对慢性应激抑郁大鼠海马激活素A和晚期糖基化终末产物受体表达的影响

发布时间：2018-01-10 11:05

本文关键词：电针对慢性应激抑郁大鼠海马激活素A和晚期糖基化终末产物受体表达的影响　出处：《中华中医药学刊》2017年01期 　论文类型：期刊论文

【摘要】：目的:观察电针对慢性应激模型大鼠海马激活素A(activin A,ACT A)和晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)蛋白表达的影响,探讨电针对慢性应激抑郁模型大鼠海马神经元的抗损伤和促生长作用。方法:将40只雄性SD大鼠随机分为4组:空白组、模型组、模型+电针组(以下简称电针组)、模型+氟西汀组(以下简称氟西汀组)。空白组正常饲养28 d,模型组、电针组、氟西汀组均采用慢性应激结合孤养方法造模28 d。电针组于造模前1 h,选取"百会"、"印堂"穴进行电针治疗,频率2 Hz,电流强度0.6 m A,每次20 min;氟西汀组于造模前1 h,给予盐酸氟西汀混悬液5 m L·kg-1灌胃治疗。通过生物素标记抗体芯片技术筛选出与神经元生长和损伤相关的差异蛋白ACT A、RAGE。结果:与空白组比较,模型组大鼠海马ACT A和RAGE蛋白表达均上调(fold change=1.240,1.356);与模型组比较,电针组、氟西汀组ACT A蛋白表达均下调(fold change=0.701,0.604)、RAGE蛋白表达均下调(fold change=0.617,0.624)。结论:电针通过下调RAGE蛋白表达水平以发挥神经元抗损伤作用,并且良性调节具有神经保护作用的ACT A,使其趋于正常水平,揭示电针可能具有促进神经元再生的作用,这些可能是针刺抗抑郁作用的相关分子机制之一。
[Abstract]:Objective: to observe the effect of electroacupuncture on hippocampal activin A in rats with chronic stress. ACT A and receptor for advanced glycation end products. To investigate the anti-injury and growth-promoting effects of electroacupuncture on hippocampal neurons in rats with chronic stress depression. Methods: forty male SD rats were randomly divided into 4 groups: blank group. Model group, model electroacupuncture group (hereinafter referred to as electroacupuncture group), model fluoxetine group (hereinafter referred to as fluoxetine group). Blank group was fed for 28 days, model group, electroacupuncture group. The rats in the fluoxetine group were treated with chronic stress combined with solitary rearing for 28 days. In the electroacupuncture group, the points of "Baihui" and "Yantang" were selected for electroacupuncture treatment at the frequency of 2 Hz at 1 hour before the model was made. Current intensity 0.6 Ma, 20 min each time; The fluoxetine group was 1 hour before the model was made. Fluoxetine hydrochloride suspension (5 mL 路kg-1) was administered intragastrically. The differential protein ACT A related to neuronal growth and injury was screened by biotin labeled antibody chip technique. Results: compared with the control group, the expression of ACT A and RAGE protein in the hippocampus of the model group were up-regulated by 1.240 and 1.356; Compared with the model group, the expression of ACT A protein in electroacupuncture group and fluoxetine group were all down-regulated. The expression of RAGE protein was down-regulated. Conclusion: electroacupuncture can play an anti-injury effect on neurons by down-regulating the expression of RAGE protein. And the benign regulation of neuroprotective ACT A to the normal level, revealing that electroacupuncture may have the role of promoting neuronal regeneration. These may be one of the molecular mechanisms related to the antidepressant effect of acupuncture.
【作者单位】：北京中医药大学针灸推拿学院;四川大学华西医院中西医结合科;郑州大学附属洛阳市中心医院;北京中日友好医院针灸科;
【基金】：国家自然科学基金项目(81173334,81202766)
【分类号】：R245.97
【正文快照】： 抑郁症又称情感性精神障碍,是一组以情绪异常低落为主的精神疾病总称,具有慢性、易复发、高致残致死率的特点。由于现今临床的抗抑郁药物均有不同程度的副反应,严重影响了疗效性和持续性。而电针治疗抑郁症以其疗效显著,不良反应不明显等特点逐渐受到人们的认可,据临床方面研

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