肾阴虚、肾阳虚模型大鼠股骨皮质骨差异蛋白质表达的分析

发布时间：2018-01-11 18:29

本文关键词：肾阴虚、肾阳虚模型大鼠股骨皮质骨差异蛋白质表达的分析　出处：《中国中西医结合杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的研究肾阴虚、肾阳虚模型大鼠股骨皮质骨差异表达的蛋白质,从功能蛋白质组角度探讨肾阴虚、肾阳虚证的实质。方法将90只大鼠按随机数字表法分为正常组、肾阴虚组和肾阳虚组,每组30只,获取造模成功且表现稳定大鼠的股骨皮质骨,分别进行皮质骨总蛋白的提取制备、双向电泳(2-DE)、凝胶图谱的获取与分析、差异蛋白的质谱分析及相关功能的生物信息学检索。结果 3组大鼠股骨皮质骨总蛋白2-DE凝胶图谱可辨识蛋白点数目均为470个,与正常组比较,肾阴虚、肾阳虚组各有12个差异蛋白,其中有6个是肾阴虚、肾阳虚组共有的差异蛋白,4个表达趋势一致,分别为表达上调的载脂蛋白A-1、α烯醇化酶,表达下调的GDP解离抑制因子β、原肌球蛋白异构体2α-3链;有2个蛋白表达趋势相反,Ⅰ型胶原α-1链在肾阴虚组中表达上调,在肾阳虚组中表达下调;线粒体热休克蛋白60在肾阴虚组中表达下调,在肾阳虚组中表达上调;肾阴虚、肾阳虚组各有6个独自的差异蛋白,其中肾阴虚组有3个蛋白表达上调,3个蛋白表达下调;肾阳虚组6个蛋白表达均下调。结论大鼠股骨皮质骨中,4个共同且表达趋势一致的差异蛋白可以视为肾阴虚、肾阳虚证的共性功能蛋白质组;2个表达趋势相反及12个肾阴虚、肾阳虚组独自的差异蛋白可归为相应证型的差异功能蛋白质组。
[Abstract]:Objective to study the expression of kidney yin deficiency and kidney yang deficiency rat model of cortical bone of femur between protein of kidney yin deficiency from functional proteomics perspective, essence of kidney yang deficiency syndrome. Methods 90 rats were randomly divided into normal group, kidney yin deficiency and kidney yang deficiency group, 30 rats in each group, the successful model of acquisition and stable rat femoral cortical bone, cortical bone were extracted for total protein by two-dimensional electrophoresis (2-DE), acquisition and analysis of gels, bioinformatics using mass spectrometry and related functions of differential proteins. Results of the 3 groups of rat femoral cortical bone 2-DE protein gels can be identified the number of protein was 470, compared with the normal group, kidney yin deficiency, kidney yang deficiency group, 12 proteins, 6 of which are yin deficiency, kidney yang deficiency group, some differential proteins, 4 expressed the same trend, respectively, on the expression of apolipoprotein A-1, Alpha enolase, downregulation of expression of GDP dissociation inhibitor beta, tropomyosin isoform 2 alpha -3 chain; 2 protein expression in the opposite directions, up regulate the expression of collagen type I alpha chain -1 in kidney yin deficiency group, expression in kidney yang deficiency group; mitochondrial heat shock protein 60 expression in kidney yin deficiency group cut, is up-regulated in kidney yang deficiency group; Yin deficiency, kidney yang deficiency group 6 alone the proteins in kidney yin deficiency group were up-regulated and 3 proteins, 3 proteins expression down regulated; kidney yang deficiency group 6 protein expression was significantly decreased. Conclusion rat femoral cortical bone, and 4 common differential expression of proteins can be regarded as the same trend of yin deficiency, common functional proteome of kidney yang deficiency syndrome; 2 and 12 opposite expression trend of yin deficiency, kidney yang deficiency group alone proteins may be attributed to the difference between the corresponding type of functional proteomics.

【作者单位】：福建中医药大学康复医学院;福建卫生职业技术学院;
【基金】：国家自然科学基金资助项目(No.81273888)
【分类号】：R22;R-332
【正文快照】： 中医学“肾主骨”理论,阐述了“肾”与“骨”之间的密切联系,认为“肾”可直接调控骨的盛衰壮健;同时,“肾者…其充在骨”,说明骨是肾功能状态的外在表现部位之一,研究者也已从肾虚状态下骨组织生理病理变化多层次论证了中医学“肾主骨”的理论[1-3]。随着蛋白质组学技术的广

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