补阳还五汤对慢性不可预知性温和应激抑郁模型小鼠行为及脑海马CA3区病理形态学影响

发布时间：2018-01-12 12:28

本文关键词：补阳还五汤对慢性不可预知性温和应激抑郁模型小鼠行为及脑海马CA3区病理形态学影响　出处：《中国实验方剂学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨补阳还五汤神经保护作用及抗抑郁机制。方法:清洁级昆明小鼠随机分为正常组,模型组,氟西汀组(3.0 mg·kg-1,ig),补阳还五汤低、中、高剂量组(9.0,18.0,27.0 g·kg-1,ig),每组10只。各给药组于每日应激前1 h ig给药,其余各组给予等体积生理盐水,共计21 d。采用慢性不可预知性温和应激法(CUMS)建立小鼠抑郁症模型,采用旷场实验(OFT),悬尾实验(TST)及体重测试(BWM)观察动物抑郁样行为;采用尼氏体染色检测海马CA3区神经元形态学变化;采用透射电镜观察海马区超微结构变化;采用TUNEL法检测海马CA3区神经元凋亡情况。结果:与正常组比较,模型组小鼠出现抑郁样行为,表现为体重减少,OFT水平及垂直运动得分减少及TST不动时间延长(P0.05,P0.01);尼氏染色及透射电镜显示海马CA3区神经元损伤及早期凋亡变化;TUNEL法显示海马CA3区神经元凋亡数量增加(P0.01)。与模型组比较,氟西汀组及补阳还五汤各剂量组小鼠抑郁样行为均有改善,表现为体重增加,OFT水平及垂直运动得分增加,TST不动时间减少(P0.05,P0.01);尼氏染色及透射电镜显示海马CA3区神经元细胞损伤减轻;TUNEL法显示海马CA3区神经元凋亡数量减少,差异有统计学意义(P0.01)。结论:补阳还五汤可能通过减轻海马CA3区神经元损伤及抑制神经元凋亡而改善小鼠抑郁样行为,具有神经保护及抗抑郁症作用。
[Abstract]:Objective: To investigate the positive also five soup neuroprotection and antidepressant mechanism. Methods: male Kunming mice were randomly divided into normal group, model group, fluoxetine group (3 mg, kg-1, Ig), Yang also five soup, low and high dose group (9.0,18.0,27.0, G, kg-1, Ig), 10 rats in each group. Each group in the daily stress 1 h before administration of Ig, the others were given an equal volume of saline, a total of 21 D. with chronic unpredictable mild stress (CUMS) method to establish a mouse model of depression, using the open field test (OFT), tail suspension test (TST) and body weight (BWM) observation test animal depression like behavior; the Nissl staining was used to detect the morphological changes of hippocampal CA3 neurons; ultrastructure of hippocampus by transmission electron microscope; TUNEL method was used to detect neuronal apoptosis in hippocampal CA3 region. Results: compared with the normal group, the model group showed depression like behavior, for weight loss, water OFT Horizontal and vertical motion score decrease and TST real time extension (P0.05, P0.01); Nissl staining and transmission electron microscope showed that the damage of hippocampus CA3 neurons and early apoptosis; TUNEL showed the number of neuron apoptosis in hippocampus CA3 region increased (P0.01). Compared with the model group, fluoxetine group and five were Buyanghuanwu Decoction each dose mice showed depression like behavior improvement, weight gain, OFT horizontal and vertical motion score increased, TST decreased the immobility time (P0.05, P0.01); Nissl staining and transmission electron microscopy showed reduced neuronal cell injury in hippocampal CA3 region; TUNEL showed the number of neuron apoptosis in hippocampal CA3 region decreased, the difference was statistically significant (P0.01) conclusion: Buyang Five Decoction can alleviate neuronal injury in hippocampal CA3 region and inhibit neuronal apoptosis and improve the depressive like behavior in mice, and has the anti depression effect of nerve protection.

【作者单位】：齐齐哈尔医学院医药科学研究院;黑龙江中医药大学中医药研究院;
【基金】：黑龙江省自然科学基金面上项目(H2015068) 齐齐哈尔医学院科研基金项目(QY2016Z-12)
【分类号】：R277.7;R-332
【正文快照】： [网络出版地址]http://www.cnki.net/kcms/detail/11.3495.R.20161027.1510.044.html[网络出版时间]2016-10-27 15:10抑郁症为心境障碍的一种临床症状,因致病危险因素多、病因不清及发病机制复杂成为精神医学科学界的难题[1],并以高患病率、高复发率、高致残率和高自杀率特点

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