疸清颗粒对肝内胆汁於积大鼠治疗的实验研究

发布时间：2018-01-12 14:04

  本文关键词：疸清颗粒对肝内胆汁於积大鼠治疗的实验研究　出处：《辽宁中医药大学》2016年硕士论文　论文类型：学位论文

  更多相关文章： 疸清颗粒 肝内胆汁淤积 病证结合

【摘要】：目的:观察疸清颗粒对于α-萘基异硫氰酸盐(ANIT)诱导具有湿热证候肝内胆汁淤积型大鼠的疗效,分析并探讨其机制。材料与方法:将60只健康清洁级Wistar大鼠随机分为空白对照组10只,以及模型组50只(模型1组、模型2组、茵栀黄颗粒组、疸清颗粒低剂量组、疸清颗粒高剂量组,每组各10只)。空白对照组每日予以0.9%生理盐水灌胃,将剩余所有大鼠放入湿热造模箱内,并每日给予50%葡萄糖和猪油脂混合液灌胃,连续3周,模拟中医湿热证候。第22日将造模组50只大鼠一次性给予2%α-萘基异硫氰酸盐溶液灌胃,复制具有湿热证候急性肝内胆汁淤积大鼠模型。模型1组造模后48h处死,留取血清、胆汁等标本备用,光镜下观察肝组织切片,成功建立模型。模型2组于造模当天起日一次予以0.9%生理盐水灌胃;剩余组别大鼠均给与相应药物灌胃给药,连续7天。第29日处死所有大鼠,采集标本。观察不同药物对模型大鼠肝功能、胆汁成分、肝脏脂蛋白X的影响,光镜下观察肝组织病理学改变,电镜观察肝细胞超微结构。结果:1.与空白组比较,两模型组大鼠一般生理状态较差;各血清生化指标(ALT、AST、ALP、TBIL、DBIL、TBA)升高明显(P0.05);脂蛋白X升高明显(P0.05);胆汁流量减少,流速减慢(P0.05),胆汁中LEC、TC降低,TBA、TB升高,均有统计学意义(P0.05);光镜下可见肝细胞肿胀、排列层次紊乱,可见大量炎性细胞和脂肪细胞;电镜下可见细胞质内线粒体肿胀破裂,呈空泡样改变。2.模型2组与模型1组比较,血清中ALT、AST、ALP、TBIL、DBIL、TBA水平和脂蛋白X含量均有所降低(P0.05);镜下肝组织损伤程度接近。3.与模型2组比较,三组治疗组大鼠一般状态好转,各血清指标以及脂蛋白均有明显下降(P0.05);胆汁流量增多,流速加快(P0.05),胆汁中LEC、TC显著升高,TBA、TB降低明显,均有统计学意义(P0.05);镜下肝组织及超微结构有明显好转。4.与茵栀黄组比较,疸清颗粒高低剂量组大鼠一般状态略好,血清中TBIL、DBIL、TBA明显降低(P0.05);镜下肝组织及超微结构恢复程度更好一些。5.疸清颗粒高低剂量组各个实验指标均无明显差异(P0.05),镜下肝组织及超微结构基本相同。结论:1.疸清颗粒可以改善湿热证候肝内胆汁淤积大鼠的生理状态。2.疸清颗粒可以改善肝功能、促进胆汁分泌排泄并减轻肝细胞病变及坏死。3.疸清颗粒对湿热证候肝内胆汁淤积症有积极的治疗作用。
[Abstract]:Objective: to observe the therapeutic effect of Jieqing granule on 伪 -naphthyl isothiocyanate ANIT-induced intrahepatic cholestasis of rats with damp-heat syndrome. Materials and methods: 60 healthy and clean grade Wistar rats were randomly divided into blank control group (n = 10) and model group (n = 50) (model 1, model 2). Yinzhihuang granule group, jinjiqing granule low dose group, jinjiqing granule high dose group, each group 10 rats. Blank control group was given 0.9% normal saline daily, the remaining rats were put into the damp-heat modeling box. In 22nd, 50 rats in the model group were given 2% 伪 -naphthyl isothiocyanate solution in a single dose of 2% 伪 -naphthyl isothiocyanate solution. The rat model of acute intrahepatic cholestasis with dampness and heat syndrome was established. The model group 1 was killed 48 hours after the model was made. The serum and bile were collected and the liver tissue sections were observed under light microscope. The model was established successfully. Group 2 was given 0.9% normal saline once a day from the same day. On 29th, all the rats were killed and the samples were collected. The effects of different drugs on liver function, bile composition and liver lipoprotein X were observed in the model rats. The pathological changes of liver tissue were observed under light microscope and the ultrastructure of hepatocytes were observed by electron microscope. Results: compared with the blank group, the general physiological state of the two model groups was worse than that of the control group. The serum biochemistry index (ALT) was significantly higher than that of the control group (P 0.05), and the level of TBA was significantly higher than that of the control group (P < 0.05). Lipoprotein X increased significantly (P 0.05); The volume of bile decreased, the velocity of flow slowed down (P 0.05), and the level of LEC-TC in bile decreased the increase of TBATB, all of which had statistical significance (P 0.05). Under the light microscope, the liver cells were swollen and arranged in disorder, and a large number of inflammatory cells and adipocytes could be seen. Under electron microscope, mitochondria in the cytoplasm were swollen and ruptured, showing vacuolar changes. Compared with model 1, the serum levels of alt ASTN and TBIL-DBIL in model 2 group were higher than those in model 1 group (P < 0.05). The level of TBA and the content of lipoprotein X decreased P0.05; The degree of liver injury was close to .3.Compared with the model group 2, the general state of the rats in the three treatment groups was improved, and the serum indexes and lipoproteins were significantly decreased (P 0.05). The bile flow rate increased, the velocity of flow increased, and the LEC-TC in bile increased significantly and TBATTB decreased significantly, all of which had statistical significance (P 0.05). Compared with the Yinzhihuang group, the rats in the high and low dosage group of jinjiqing granule were in good general condition, and the serum TBIL-DBIL was better than that in the control group. TBA decreased significantly (P 0.05); The degree of recovery of liver tissue and ultrastructure was better under microscope. There was no significant difference in each experimental index between high and low dosage groups of jinjiqing granules (P0.05). The liver tissue and ultrastructure were basically the same under the microscope. Conclusion 1. Jinjiqing granule can improve the physiological state of intrahepatic cholestasis rats with damp-heat syndrome .2. jinjiqing granule can improve liver function. Promoting bile excretion and reducing hepatocyte lesion and necrosis .3.Jinjiqing granule has a positive therapeutic effect on intrahepatic cholestasis of damp-heat syndrome.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259
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本文编号：1414557