定喘汤对呼吸道合胞病毒感染儿童及大鼠的临床与基础研究

发布时间：2018-01-14 09:09

本文关键词：定喘汤对呼吸道合胞病毒感染儿童及大鼠的临床与基础研究　出处：《大连医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：第一部分定喘汤对呼吸道合胞病毒诱导大鼠肺炎免疫细胞因子作用的研究目的:本研究通过建立呼吸道合胞病毒感染幼龄大鼠肺炎模型,运用Elisa方法检测分析RSV感染后各组大鼠血清中免疫细胞因子(IFN-γ、IL-4、IFN-γ/IL-4、IL-10、IL-13、IL-33、IL-17)的变化,从而反应RSV感染后Th1/Th2相关细胞因子失衡的机制,进一步研究定喘汤对RSV感染后大鼠体内免疫细胞因子的影响,阐明定喘汤防治RSV感染的作用机制,为减少RSV感染后哮喘发病提供科学的依据。方法:1.将36只Wistar大鼠随机分为对照组、RSV感染组、利巴韦林组、定喘汤组,每组各9只,除对照组外,余各组均用RSV(Long株)病毒予大鼠滴鼻,滴鼻后对照组和RSV感染组以等体积的生理盐水灌胃,各干预组分别给于相应的药物灌胃。通过观察各组大鼠的一般状态、肺指数、病理改变情况等指标,来评估定喘汤对大鼠RSV肺炎的防治作用。2.运用酶联免疫吸附法(Elisa)对各组大鼠的血清免疫细胞因子IFN-γ、IL-4、IL-10、IFN-γ/IL-4、IL-13、IL-33、IL-17 水平进行检测。结果:1.RSV感染后第7天,与对照组大鼠相比,RSV感染组大鼠一般状态及肺组织病理情况均较差,肺指数增加有显著差异(P0.05);与RSV感染组大鼠比较,定喘汤组及利巴韦林组大鼠一般状态及肺组织病理情况均得到改善,肺指数降低有显著差异(P0.05);利巴韦林组大鼠与定喘汤组大鼠比较,无显著差异(P0.05)。2.血清中各细胞因子表达结果显示:与对照组比较,RSV感染组的IFN-γ和IFN-γ/IL-4降低及其余各项指标均升高具有显著统计学意义(P0.05);与RSV感染组比较,定喘汤组 IFN-γ 和 IFN-γ/IL-4 升高,IL-4、IL-10、IL-13、IL-33、IL-17均降低具有显著统计学意义(P0.05);与利巴韦林组比较,定喘汤组IFN-γ升高,IL-33降低具有显著统计学意义(P0.05)。结论:1.定喘汤能减轻RSV感染后大鼠肺部病理损伤,有效防止RSV感染。2.RSV感染大鼠后血清中Th1相关细胞因子下降,Th2相关细胞因子上升,机体出现Th1/Th2平衡漂移。3.定喘汤干预RSV感染的大鼠后可以明显抑制Th2相关细胞因子反应,增强Th1相关细胞因子反应,对RSV感染后Th1/Th2细胞因子的失衡具有双向调节作用。第二部分毛细支气管炎住院患儿免疫细胞因子水平的研究目的:从Th1/Th2平衡角度探讨毛细支气管炎住院患儿血清中免疫细胞因子(IFN-γ、IL-4、IFN-γ/IL-4、IL-10、IL-13、IL-33、IL-17)水平的变化,来阐明毛细支气管炎的发病机制,为预防其日后发展为哮喘提供理论依据。方法:1.采用前瞻性的研究方法,收集2016年9月到2017年2月于大连市儿童医院呼吸科住院小于2岁毛细支气管炎患儿为毛细支气管炎组。同时选择同期体检健康同龄儿童为正常组,观察两组儿童免疫细胞因子的指标变化。2.运用酶联免疫吸附法(Elisa)对正常组患儿及毛细支气管炎组患儿血清IFN-γ、IL-4、IFN-γ/IL-4、IL-10、IL-13、IL-33、IL-17 水平进行检测。结果:血清中各细胞因子表达结果显示:与对照组比较,毛细支气管炎组的IL-4、IL-10、IL-13、IL-33、IL-17水平升高有显著统计学意义(P0.05)。结论:小儿毛细支气管炎发病与相关免疫细胞因子水平的变化密切相关,机体处于Th1/Th2免疫失衡状态。
[Abstract]:Objective to study the first part Dingchuan Decoction on rats induced by respiratory syncytial virus pneumonia immune cell factor: This study through the establishment of respiratory syncytial virus infection in young rat pneumonia model, using Elisa method to detect RSV infection after analysis of immune cells in serum factor (IFN- gamma, IL-4, IFN- y /IL-4, IL-10. IL-13, IL-33, IL-17) changes, and reaction mechanism of RSV infection after the imbalance of Th1/Th2 related cytokines, further study on the influence of Dingchuan Decoction on cytokines in rats after RSV infection, to clarify the mechanism of dingchuantang preventing RSV infection, to provide a scientific basis for reducing the incidence of asthma after RSV infection. Methods: 1. 36 Wistar rats were randomly divided into control group, RSV infection group, Leigh Bhave Lin group, dingchuantang group, 9 rats in each group, except the control group, the other groups were RSV (Long strain) virus to rat nasal drip after intranasal control Group and RSV infection group with equal volume of saline, the intervention group were given intragastric administration with corresponding drugs. The general state of rats were observed in the lung index, pathological changes and other indicators, to evaluate the Dingchuan Decoction on rat RSV pneumonia. The prevention and cure effect of.2. by enzyme-linked immunosorbent method (Elisa) serum cytokines IFN- in rats, IL-4, IL-10, IL-13, IFN- y /IL-4, IL-33, IL-17 levels were detected. Results: seventh days after 1.RSV infection, compared with control group, RSV infection and pathological condition of the general condition and lung tissue of rats were poor, lung there are significant differences in index increased (P0.05); and RSV infection group rats, dingchuantang group and ribavirin group rats general state and lung tissue pathological conditions were improved, the lung index decreased significantly (P0.05); compared with ribavirin group rats and dingchuantang group rats, no significant difference (P 0.05) the results showed that the expression of.2. in serum: compared with the control group, RSV infection group, IFN- gamma and IFN- gamma /IL-4 decreased and the rest indexes were increased significantly (P0.05); compared with RSV infection group, dingchuantang group IFN- IFN- and gamma gamma /IL-4 increased, IL-4, IL-10, IL-13, IL-33, IL-17 decreased significantly (P0.05); compared with ribavirin group, dingchuantang group IFN- y increased, there was a statistically significant decrease in IL-33 (P0.05). Conclusion: the lung injury rats reduced after RSV infection 1. dingchuantang, effectively prevent RSV infection in serum Th1 related cytokines decreased rats infected with.2.RSV, increase of Th2 cytokines, immune balance of Th1/Th2 drift.3. dingchuantang RSV infection in rats after intervention can significantly inhibit the reaction of Th2 related cytokines, enhance the reaction of Th1 related cytokines in RSV infected Th1/ The imbalance of Th2 cytokine has bidirectional regulation effect. The research purpose of the second part of bronchiolitis hospitalized children: the level of immune cytokine on immune cells in serum of children with bronchiolitis hospitalized in Th1/Th2 from the perspective of balance factor (IFN- gamma, IL-4, IL-10, IFN- y /IL-4, IL-13, IL-33, IL-17) level changes, to clarify the pathogenesis for the prevention of bronchiolitis, the day after the development to provide a theoretical basis for asthma. Methods: 1. using prospective research method, from September 2016 to February 2017 in the Department of respiration of Dalian city children's hospital less than 2 years old children with bronchiolitis as bronchiolitis group. At the same time choose the healthy children of the same age as normal group, observe two groups of children cytokines index changes of.2. by enzyme linked immunosorbent assay (Elisa) in normal children and children with IFN- bronchiolitis serum gamma, IL-4, IL-10, IFN- y /IL-4, IL-13, IL-33, IL-17 levels were detected. Results: the results showed that the expression of cytokines in serum: compared with the control group, bronchiolitis group IL-4, IL-10, IL-13, IL-33, IL-17 levels were statistically significant (P0.05). Conclusion: changes in children with bronchiolitis and the related immune cytokine level is closely related to the body in Th1/Th2 immune imbalance.

【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R272

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