艾灸“肺俞”“肾俞”对哮喘大鼠外周血T细胞及血清白介素的影响

发布时间：2018-01-14 12:10

本文关键词：艾灸“肺俞”“肾俞”对哮喘大鼠外周血T细胞及血清白介素的影响　出处：《针刺研究》2017年02期 　论文类型：期刊论文

【摘要】：目的:观察艾灸"肺俞""肾俞"对哮喘大鼠外周血T细胞及血清白介素的影响,探讨艾灸治疗哮喘的免疫学机制。方法:SD大鼠随机分为正常组、模型组、艾灸组,每组各10只。以卵白蛋白加氢氧化铝腹腔注射并给予雾化激发法制备哮喘模型。艾灸组大鼠间接灸双侧"肺俞""肾俞"穴,每次30min,每日1次,共治疗14d。用HE染色行肺组织病理学观察,用流式细胞仪检测全血CD 3~+、CD 4~+、CD 8~+T细胞水平,用酶联免疫吸附法检测血清免疫球蛋白E(IgE)、白细胞介素-1β(IL-1β)、IL-1受体拮抗剂(IL-1Ra)水平。结果:模型组大鼠支气管壁肌层增厚,黏膜水肿,大量炎性细胞浸润,部分肺泡结构破坏明显;艾灸组大鼠肺泡壁结构完整,细支气管形态规则,管腔内无黏液栓形成及上皮细胞脱落等现象。模型组全血CD 8~+、CD3~+T细胞水平及血清IgE、IL-1β含量较正常组显著升高(P0.01)。艾灸组CD 8~+T细胞及血清IgE、IL-1β水平较模型组显著降低(P0.05,P0.01),CD 4~+T细胞水平、血清IL-1Ra水平较模型组显著升高(P0.01,P0.05)。结论:艾灸大鼠"肺俞""肾俞"穴能降低血清IgE、IL-1β水平,升高血清IL-1Ra水平,达到治疗哮喘的目的。
[Abstract]:Objective: to observe the effect of moxibustion "Feshu" and "Shenshu" on peripheral blood T cells and serum interleukin in asthmatic rats, and to explore the immunological mechanism of moxibustion in treating asthma. Methods: the rats were randomly divided into normal group and model group. In moxibustion group, 10 rats in each group were injected with ovalbumin and aluminum hydroxide intraperitoneally and induced by atomization. The rats in moxibustion group had indirect moxibustion of both sides of "Fesu" and "Shenshu" for 30 minutes each time. The lung histopathology was observed by HE staining and the whole blood CD3 ~ + CD4 ~ + CD8 ~ T cells were detected by flow cytometry. Elisa was used to detect the levels of serum immunoglobulin (IgE) and interleukin-1 尾 (IL-1 尾). Results: in the model group, the muscular layer of bronchial wall was thickened, mucosal edema, inflammatory cell infiltration and partial destruction of alveolar structure were observed. In the moxibustion group, the alveolar wall was intact, the bronchioles were regular, there were no mucus embolus formation and epithelial cells shedding in the lumen, and the whole blood of the model group was CD8 ~. The level of CD3 ~ T cells and the content of serum IgE ~ T IL-1 尾 were significantly higher than those of the normal group (P 0.01). The CD8 ~ T cells and serum IgE in moxibustion group were significantly higher than those in the normal group. Compared with the model group, the level of IL-1 尾 was significantly lower than that of the model group, and the serum IL-1Ra level was significantly higher than that of the model group. Conclusion: moxibustion at "Feshu" and "Shenshu" can decrease the level of serum IgE and increase the level of serum IL-1Ra and achieve the purpose of treating asthma.
【作者单位】：安徽中医药大学针灸骨伤临床学院;
【基金】：安徽中医学院科研项目(No.2012zr 010)
【分类号】：R245
【正文快照】： 支气管哮喘是由嗜酸性粒细胞、T淋巴细胞、肥大细胞等多种细胞共同参与的慢性气道炎性反应,是临床常见的慢性呼吸道疾病之一。目前哮喘的治疗仍然以调节免疫功能和控制气道炎性反应为主。CD 3+T细胞是所有的T淋巴细胞总称,其数量反映了机体的整体免疫状态[1],可分为CD 4+和CD8

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