经筋微创松解疗法对膝骨关节炎兔软骨细胞凋亡的抑制作用及机制

发布时间：2018-01-15 21:10

本文关键词：经筋微创松解疗法对膝骨关节炎兔软骨细胞凋亡的抑制作用及机制　出处：《山东医药》2017年21期 　论文类型：期刊论文

【摘要】：目的观察经筋微创松解疗法对膝骨关节炎兔软骨细胞凋亡的抑制作用,并探讨其作用机制。方法将30只健康白兔随机均分为正常组、模型组与治疗组。模型组和治疗组采用膝关节制动法制备关节炎动物模型。治疗组给予经筋微创松解疗法治疗4周,其余两组不做处理。耳缘静脉空气栓塞法处死实验动物,取其胫骨软骨组织及膝关节液。用TUNEL细胞凋亡检测法测算膝关节软骨细胞凋亡指数,用ELISA法检测各组膝关节液中白细胞介素1β(IL-1β)、转化生长因子β1(TGF-β1)和肿瘤坏死因子α(TNF-α),Western blotting技术检测膝关节软骨组织中氨基末端激酶(JNK)和细胞外信号调节激酶(ERK)蛋白表达。结果模型组膝关节软骨细胞凋亡指数及膝关节液中IL-1β、TNF-α、TGF-β1水平,ERK、JNK蛋白相对表达量与对照组比较,P均0.05;治疗组软骨细胞凋亡指数及膝关节液中IL-1β、TNF-α、TGF-β1水平,ERK、JNK蛋白相对表达量与模型组比较,P均0.05。结论经筋微创松解疗法可抑制膝骨关节炎兔软骨细胞凋亡,其作用机制可能为降低关节液中炎性因子水平及软骨细胞MAPKs信号通路相关蛋白表达。
[Abstract]:Objective to observe the inhibitory effect of minimally invasive release therapy of meridian on chondrocyte apoptosis in rabbits with knee osteoarthritis and its mechanism. Methods 30 healthy rabbits were randomly divided into normal group. The model group and the treatment group. The model group and the treatment group using the knee joint immobilization method to make arthritis animal model, the treatment group was given meridian minimally invasive release therapy for 4 weeks. The other two groups were not treated. The experimental animals were killed by auricular vein air embolization. The tibial cartilage tissue and knee joint fluid were taken. The apoptosis index of chondrocytes of knee joint was measured by TUNEL cell apoptosis assay. The levels of IL-1 尾, TGF- 尾 1, TGF- 尾 1 and TNF- 伪 in the knee joint fluid of each group were detected by ELISA method. Detection of amino-terminal kinase (Western) and extracellular signal-regulated kinase (ERK) in articular cartilage by Western blotting. Results the apoptotic index of chondrocytes and IL-1 尾 in knee joint fluid in the model group. Compared with the control group, the relative expression of TNF- 伪 TGF- 尾 1 and ERKN JNK protein were 0.05; The apoptotic index of chondrocytes and the relative expression of IL-1 尾 -TNF- 伪 TGF- 尾 1 in the treatment group were compared with those in the model group. Conclusion minimally invasive release therapy of meridians can inhibit chondrocyte apoptosis in rabbits with knee osteoarthritis. The mechanism may be to reduce the level of inflammatory factors in articular fluid and the expression of MAPKs signaling pathway related protein in chondrocytes.
【作者单位】：广州军区广州总医院;广州中医药大学;
【基金】：国家自然科学基金资助项目(815738831008646) 广东省科技计划项目应用型科技研发专项(2015B02033011)
【分类号】：R274
【正文快照】： 膝骨关节炎是一种关节软骨慢性退变导致的骨关节疾病,多发于中老年人,临床主要以软骨退变和滑膜炎症为特征[1]。性别、体质量、年龄、遗传等全身因素和关节力学、细胞炎症或凋亡因子、软骨细胞凋亡以及降解酶等局部因素综合作用,导致骨关节软骨发生代谢、生化环境和结构改变,

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