电针预处理对大鼠脑缺血再灌注损伤后脑梗死体积及血清TNF-α、IL-10含量的影响

发布时间：2018-01-17 01:01

本文关键词：电针预处理对大鼠脑缺血再灌注损伤后脑梗死体积及血清TNF-α、IL-10含量的影响　出处：《中国针灸》2017年10期 　论文类型：期刊论文

【摘要】：目的:观察电针预处理对大鼠脑缺血再灌注损伤后脑梗死体积及血清肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)含量的影响,探索预防脑卒中的方法。方法:采用随机数字表将36只大鼠随机分为假手术组、模型组、电针预处理组共3组,每组12只。这3组内又分为再灌注12 h和再灌注24 h 2个亚组,每个亚组6只大鼠。电针预处理组大鼠造模前给予连续2周的电针干预。模型组和电针预处理组采用改良Longa线栓法制备大鼠脑缺血再灌注模型。再灌注12 h、24 h,采用改良行为学评分量表评估各组大鼠神经功能缺损程度,TTC法观测脑梗死体积,酶联免疫分析(ELISA)法检测血清TNF-α、IL-10含量。结果:再灌注12 h、24 h,与模型组比较,电针预处理组神经功能缺损评分均明显较低(均P0.05),脑梗死体积均明显较低(均P0.05)。再灌注12 h、24 h,与假手术组比较,模型组TNF-α、IL-10含量均较高(均P0.05);再灌注12 h,与模型组比较,电针预处理组血清TNF-a含量较低(P0.05),而血清IL-10含量较高(P0.05);再灌注24 h,与模型组比较,电针预处理组血清TNF-a、IL-10含量均较低(P0.05)。结论:电针预处理可以改善大鼠脑缺血再灌注损伤后神经功能缺损,缩小脑梗死体积,发挥脑保护作用,其机制可能与电针能调节脑缺血再灌注急性期外周循环血中促炎因子TNF-a与抗炎因子IL-10间动态平衡,对抗炎性反应加剧有关。
[Abstract]:Objective: to observe the effect of electroacupuncture preconditioning on the volume of cerebral infarction and the contents of serum tumor necrosis factor- 伪 (TNF- 伪) and interleukin-10 (IL-10) after cerebral ischemia-reperfusion injury in rats. Methods: 36 rats were randomly divided into three groups: sham operation group, model group and electroacupuncture preconditioning group. There were 12 rats in each group. The 3 groups were divided into two subgroups: reperfusion for 12 h and reperfusion for 24 h. Six rats in each subgroup were treated with electroacupuncture for 2 weeks before modeling. The model group and electroacupuncture preconditioning group were used to establish cerebral ischemia reperfusion model by modified Longa thread embolization. 12. H. At 24 h, the size of cerebral infarction was measured by TTC method and serum TNF- 伪 was detected by enzyme linked immunosorbent assay (Elisa). Results: compared with the model group, the nerve function defect score of the electroacupuncture preconditioning group was significantly lower than that of the model group (P 0.05). The volume of cerebral infarction was significantly lower (P 0.05). Compared with sham-operation group, the TNF- 伪 IL-10 content in model group was higher than that in sham operation group (P 0.05). After 12 hours of reperfusion, compared with the model group, the serum TNF-a content was lower in the electroacupuncture pretreatment group than that in the model group, while the serum IL-10 content was higher in the electroacupuncture preconditioning group than that in the model group. Compared with model group, serum TNF-a in electroacupuncture preconditioning group was compared with that in model group for 24 h after reperfusion. Conclusion: electroacupuncture pretreatment can improve the nerve function defect, reduce the volume of cerebral infarction and play a protective role in cerebral ischemia reperfusion injury in rats. The mechanism of electroacupuncture may be related to the regulation of dynamic balance between pro-inflammatory factor TNF-a and anti-inflammatory factor IL-10 in peripheral circulation blood during acute cerebral ischemia-reperfusion period, and may be related to the aggravation of anti-inflammatory reaction.
【作者单位】：黑龙江中医药大学;黑龙江中医药大学附属第二医院;
【基金】：国家自然科学基金:81503666 黑龙江省自然科学基金:QC 2015103 哈尔滨市科技创新人才专项基金(青年后备人):2014 RFQGJ 150 黑龙江中医药大学领军人才计划项目:2012 RCL 02;黑龙江中医药大学研究生创新科研项目:yjscx 2016037
【分类号】：R245.9
【正文快照】： 脑缺血再灌注后,机体免疫系统激活,包括肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、白介素-10(interleukin-10,IL-10)在内的免疫细胞因子启动并介导瀑布式免疫炎性反应,参与脑缺血再灌注损伤的发生、发展及修复过程。如何恢复各细胞因子的网络平衡关系,调整机体紊

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