艾灸对慢性心力衰竭大鼠心功能及心肌组织TNF-α、NF-κB表达水平的影响

发布时间：2018-01-17 03:15

本文关键词：艾灸对慢性心力衰竭大鼠心功能及心肌组织TNF-α、NF-κB表达水平的影响　出处：《中国中西医结合杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的观察艾灸对慢性心力衰竭(chronic heart failure,CHF)大鼠心功能及心肌组织肿瘤坏死因子-α(TNF-α)mRNA、核因子-κB(NF-κB)表达水平的影响,探讨艾灸对CHF心肌保护作用机制。方法采用盐酸多柔比星隔日腹腔注射的方法构建CHF模型,将造模成功的28只大鼠随机分为模型组、艾灸组、西药组、灸药(艾灸加西药)组,每组7只。艾灸组给予温和灸双侧肺俞、心俞,每日1次,每次15 min;西药组灌服卡托普利混悬液(25 mg/kg),每日1次;灸药组给予卡托普利混悬液(25 mg/kg)灌服结合温和灸双侧肺俞、心俞,每日1次,各组均连续3周。检测大鼠心率(HR)、心输出量(CO)、左心室收缩压(LVSP)、左心室舒张末压(LVEDP)、左室内压最大上升或下降速率(±dp/dtmax)、心肌组织NF-κB活性及TNF-αmRNA表达水平。结果与正常大鼠比较,建模大鼠的行为体征改变明显,心功能指标明显异常(P0.01),心肌组织HE染色结果符合CHF表现。与正常组比较,模型组大鼠HR、LVEDP升高,CO、LVSP、+dp/dtmax和-dp/dtmax降低(P0.01),心肌组织TNF-αmRNA表达及NF-κB蛋白结合活性明显升高(P0.01);与模型组比较,艾灸组、西药组及灸药组CO、LVSP、±dp/dtmax均升高,HR、LVEDP明显降低(P0.01),TNF-αmRNA表达及NF-κB核蛋白DNA结合活性降低(P0.01);与艾灸组和西药组比较,灸药组HR、LVEDP降低,CO、LVSP、±dp/dtmax升高明显(P0.05),且TNF-αmRNA表达降低明显(P0.05)。结论艾灸联合西药改善大鼠心功能指标,降低TNF-αmRNA表达方面优于单用艾灸及西药,可能通过降低TNF-αmRNA表达,抑制心肌组织NF-κB核蛋白的DNA结合活性,抑制免疫炎症反应,从而改善CHF症状。
[Abstract]:Objective to observe the effect of moxibustion on cardiac function and tumor necrosis factor- 伪 (TNF- 伪) mRNA in myocardium of rats with chronic heart failure. Effects of NF-魏 B on the expression of NF- 魏 B, to explore the protective mechanism of moxibustion on myocardium of CHF. Methods doxorubicin hydrochloride was injected intraperitoneally every other day to construct CHF model. Twenty-eight rats were randomly divided into three groups: model group, moxibustion group, western medicine group and moxibustion medicine (moxibustion plus western medicine) group, 7 rats in each group. The moxibustion group was given mild moxibustion on both sides of Fesu and Xinshu once a day. 15 mins each time; The western medicine group was given captopril suspension 25 mg / kg, once a day; The moxibustion group was given captopril suspension (25 mg / kg) combined with mild moxibustion of bilateral Feshu and Xinshu once a day for 3 weeks. Heart rate and heart output were measured. Left ventricular systolic pressure (LVSPN), left ventricular end-diastolic pressure (LVEDPN), and left ventricular pressure (LVSP) increased or decreased at a maximum rate (卤DP / dtmax). Results compared with normal rats, the activity of NF- 魏 B and the expression of TNF- 伪 mRNA were significantly changed in the model rats, and the cardiac function indexes were significantly abnormal (P 0.01). The results of HE staining in myocardial tissue were consistent with the findings of CHF. Compared with the normal group, the LVEDP in the model group was higher than that in the control group. Dp/dtmax and -dp / dtmax decreased the expression of TNF- 伪 mRNA and the binding activity of NF- 魏 B protein. Compared with the model group, the moxibustion group, western medicine group and moxibustion group were significantly lower than those in the moxibustion group (P 0.01). The expression of TNF- 伪 mRNA and the DNA binding activity of NF- 魏 B nuclear protein decreased P0.01; Compared with moxibustion group and western medicine group, LVEDP in moxibustion group decreased COLVSPand 卤dp/dtmax increased significantly (P0.05). Conclusion moxibustion combined with western medicine can improve the cardiac function of rats and decrease the expression of TNF- 伪 mRNA, which is better than that of moxibustion and western medicine alone. The expression of TNF- 伪 mRNA and the DNA binding activity of NF- 魏 B nucleoprotein in myocardium may be inhibited, and the immune inflammatory response can be inhibited, thus improving the symptoms of CHF.
【作者单位】：安徽中医药大学中医学院;安徽中医药大学研究生院;安徽省立医院中医科;安徽中医药大学第二附属医院肿瘤科;
【基金】：国家自然基金面上资助项目(No.81574084,No.81273856) 安徽省自然基金面上资助项目(No.1608085MH230) 安徽省高校科研创新平台建设项目(No.2015TD 033)
【分类号】：R245
【正文快照】： 慢性心力衰竭(chronic heart failure,CHF),是由多种原因引起的心室充盈或射血能力障碍,继而导致心输出量减少,循环淤血的一种复杂的临床综合征,多见于各种心血管疾病的后期,病发率高,严重影响患者的生活质量和生命,属于临床最重要的心血管疾病之一[1,2]。目前临床治疗CHF的药

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