藏药“蒂达”的6种主要基原植物提取物对急性肝内胆汁淤积小鼠药理作用的比较研究

发布时间：2018-01-17 03:11

本文关键词：藏药“蒂达”的6种主要基原植物提取物对急性肝内胆汁淤积小鼠药理作用的比较研究　出处：《中药新药与临床药理》2017年03期 　论文类型：期刊论文

【摘要】：目的比较藏药"蒂达"(藏茵陈)的6种主要基原植物小伞虎耳草、篦齿虎耳草、印度獐牙菜、川西獐牙菜、大籽獐牙菜和椭圆叶花锚提取物对急性肝内胆汁淤积小鼠的药理作用,为其品种整理、质量控制、临床应用及资源综合开发利用提供药理学依据。方法采用α-异硫氰酸萘酯(ANIT)诱导小鼠急性肝内胆汁淤积型肝损伤模型,以各药材提取物进行干预,熊去氧胆酸(UDCA)为对照,检测小鼠血清ALT、AST、TBA、TBIL、DBIL和IBIL水平,HE染色观察肝组织病理变化。结果 6种药材提取物均能不同程度地改善ANIT引起的肝损伤,各药材提取物组在肝小叶结构、肝细胞病变等方面病理改变均相对减轻,其中,以小伞虎耳草组、篦齿虎耳草组、椭圆叶花锚组和大籽獐牙菜组明显;各药材提取物组小鼠血清中ALT和AST的水平均明显降低,小伞虎耳草、印度獐牙菜和川西獐牙菜组小鼠血清中TBA的水平均明显降低,小伞虎耳草、篦齿虎耳草、川西獐牙菜和大籽獐牙菜组小鼠血清中TBIL、DBIL和IBIL的水平均明显降低。结论小伞虎耳草和篦齿虎耳草提取物在降酶和退黄两方面效果最为显著,且总体强于阳性药熊去氧胆酸,是开发针对胆汁淤积型肝病治疗药物的优势药材品种;6种药材提取物均可明显改善急性肝内胆汁淤积小鼠肝组织损伤及肝功能,说明藏医将其归为同一类药材存在一定的合理性,而其药理作用差异则提示藏药"蒂达"各基原药材间在功效、物种亲缘关系及化学成分方面存在差异和相关关系;本研究为"蒂达"药材的品种整理、质量控制、临床应用和资源综合开发利用提供了药理学依据,并为其他类似复杂基原藏药的品种整理等研究提供了参考。
[Abstract]:Objective to compare the six main basic plants of Tibetan medicine "Tida", Saxifraga grisea, Swertia davidiana and Swertia mustiflorum. The pharmacological effects of Swertia davidiflora and Anchorage ovale extracts on acute intrahepatic cholestasis mice were studied. Methods the acute intrahepatic cholestatic liver injury model in mice was induced by 伪 -naphthalene thiocyanate. The serum levels of TBILIL and IBIL were measured in mice with ursodeoxycholic acid (UDCA). Results the liver injury induced by ANIT was improved in all the 6 kinds of extracts, and the structure of hepatic lobule was found in each extract group. The pathological changes of liver cell were relatively alleviated, among which, the group of Saxifraga vulgaris, the group of Saxifraga grisea, the group of Anchorage of elliptical leaf and the group of Swertia davidiflora were obvious. The levels of ALT and AST in the serum of mice in the extract group were significantly lower than those in the control group. The levels of TBA in the serum of the mice in the three groups were significantly lower than those in the control group. TBIL in serum of mice in the groups of Saxifraga grisea Swertia and Swertia davidii. The levels of DBIL and IBIL were significantly decreased. Conclusion the extract of Saxifraga vulgaris and Saxifraga grisea has the most significant effect in reducing enzyme and reducing yellow, and it is stronger than ursodeoxycholic acid in general. Is the development of cholestatic liver disease treatment drugs for the advantage of the variety; The six kinds of extracts could obviously improve the liver tissue injury and liver function in mice with acute intrahepatic cholestasis, which indicated that it was reasonable for Tibetan medicine to classify them into the same kind of medicine. The difference of pharmacological action indicated that there were differences and correlations in efficacy, species relationship and chemical composition among the original medicinal materials of Tibetan medicine "Tida". This study provides pharmacological basis for the variety arrangement, quality control, clinical application and comprehensive exploitation and utilization of Tida, and provides a reference for the variety arrangement of other similar complex primitive Tibetan medicines.
【作者单位】：江西中医药大学;江西民族传统药现代科技与产业发展协同创新中心;重庆市中药研究院;
【基金】：国家自然科学基金(81360675) 国家科技重大专项“重大新药创制”子课题(2012ZX09103201-050)
【分类号】：R29
【正文快照】： 胆汁淤积型肝病是以胆汁淤积为主要表现的临床常见疾病,是各种原因引起的胆汁形成、分泌和(或)胆汁排泄异常引起的肝脏病变。熊去氧胆酸(UDCA)为治疗胆汁淤积型肝病的首选药物[1-2]。“蒂达”(藏茵陈)为藏医临床治疗肝胆疾病使用频率最高的一类药材,《晶珠本草》[3]中记载:“

【相似文献】