古日古木-7有效部位的制备、保肝活性、定量分析及锁阳产品的研制

发布时间：2018-01-17 14:24

  本文关键词：古日古木-7有效部位的制备、保肝活性、定量分析及锁阳产品的研制　出处：《内蒙古大学》2016年硕士论文　论文类型：学位论文

  更多相关文章： 古日古木-7 保肝 活性部位 活性成分 锁阳 三萜咀嚼片 膳食纤维

【摘要】：第一部分古日古木-7是一种重要的传统蒙药复方,被广泛应用于肝病的治疗。本研究探讨了古日古木-7活性部位(大孔树脂分离的甲醇洗脱部分,MF)对四氯化碳导致小鼠肝损伤的保护作用,并用超高效液质联用仪(UPLC-MS)分析了小鼠血清中的胆酸含量的变化以及MF中的活性成分。结果表明,连续给MF(66.132.264 mg/kg)四天,每天一次可以抑制四氯化碳(CCl4)诱导的小鼠肝脏组织病理学变化和血清转氨酶的改变,并且具有一定的量效关系(组间比较用t检验(P0.05具显著差异；P0.01具极显著差异),结果发现丙氨酸转氨酶(ALT)为2766.0±1909.7U/L、1929.0±799.8U/L.1144.0 ±787.2U/L(较模型组2461.8±1072.7U/L有显著差异,P0.05)；天冬氨酸转氨酶(AST)为2482.8±1853.3U/L.1748.0±694.8U/L. 1173±785.3U/L(较模型组2506.6±1140.7U/L有极显著差异,P0.01)。MF给药组的超氧化物歧化酶(SOD)较四氯化碳组(75.75 ±5.37U/mg protein)有所增加：82.46±15.48U/mg protein.93.39± 40.91 U/mg protein.108.19±30.32U/mg protein(P0.01),但丙二醛(MDA)较四氯化碳组(44.32±16.68nmol/mg protein)水平有所降低：19.31 ± 6.58nmol/mg protein (P0.01).8.58±1.11 nmol/mg protein (P0.01).7.68±1.95nmol/mg protein (P0.01)。MF组和水飞蓟素组的小鼠血清中,4种牛磺酸结合型胆汁酸含量明显高于空白组。定量分析显示,MF含有大量的鞣花酸(297.97mg/g)、木犀草素与其糖苷(35.10mg/g)、芹菜素与其糖苷(30mg/g)、熊果酸(14.91mg/g)、bidenoside C (8.75 mg/g)、原花青素(15.64mg/g的原花青素A2相当量)。与古日古木-7相比,MF的有效量和化合物的数量都有所减少,为开发用量少并且易于质量控制的新保肝药物打下了基础。第二部分锁阳(Cynomorium songaricium)是一种无叶绿素,全寄生的植物,主要集中于我国的西北部。本研究以锁阳为原料,制备锁阳产品。将锁阳粉碎成豌豆大小的颗粒,用95%的乙醇提取锁阳中的三萜成分,以含有三萜成分的乙醇提取物制备锁阳三萜咀嚼片,确定配方为：30%锁阳三萜、15%奶粉、17%糖粉、27%玉米淀粉、10%微晶纤维素、0.22%甘草酸、0.78%柠檬酸等；将乙醇提取后的残渣再用半胱氨酸加维生素C或者水在60℃下浸泡提取48 h,将所得残渣清洗、烘干粉碎后过40目筛制得的两种膳食纤维,测定两种膳食纤维的物理性质,结果发现,由半胱氨酸酸溶液处理的所得的膳食纤维的持水性为9.20 g水/g样品,溶胀性为7.5 mL/g、水处理的膳食纤维这两种性质分别是5.83g水/g样品；4.4 mL/g,与水处理的相比,半胱氨酸酸溶液处理后所得的膳食纤维质量明显较优。
[Abstract]:The first part is an important traditional Mongolian medicine compound, which is widely used in the treatment of liver disease. The active site (methanol elution part of macroporous resin) was studied in this study. The protective effect of MF on liver injury induced by carbon tetrachloride in mice. The changes of serum cholic acid and the active components in MF were analyzed by UPLC-MS. MF(66.132.264 mg / kg for four days could inhibit the liver histopathological changes and serum aminotransferase changes induced by CCL _ 4). And there was a certain dose-effect relationship (significant difference was found between the two groups by t test (P0.05). The results showed that alanine aminotransferase (alt) was 2766.0 卤1909.7 U / L. 1 929.0 卤799.8 U / L 1144.0 卤787.2 U / L (P 0.05) as compared with 2461.8 卤1072.7 U / L in the model group; Aspartate transaminase (AST) was 2482.8 卤1853.3 U / L. 1748.0 卤694.8U / L, 1173 卤785.3 U / L. There was a significant difference between the model group and the model group (2506.6 卤1140.7 U / L). Compared with carbon tetrachloride group, the superoxide dismutase (SOD) of P0.01U 路MF group was 75.75 卤5.37 U / mg protein. Increased: 82.46 卤15.48 U / r mg protein.93.39 卤40.91 U / r mg. Protein.108.19 卤30.32 U / mg protein. P0.01). But MDAs were 44.32 卤16.68nmol / mg protein in carbon tetrachloride group. There was a decrease in the level of: 19.31 卤6.58nmol / mg protein protein (P 0.01) .8.58 卤1.11 nmol/mg protein (. P0.01nmol.7.68 卤1.95nmol / mg protein in the serum of mice in the P0.01ML-MF group and silymarin group. The content of four taurine conjugated bile acids was significantly higher than that of the blank group. The quantitative analysis showed that there was a large amount of tannic acid in the MF (297.97 mg / g). Luteolin and its glycoside 35.10 mg / g, apigenin and its glycoside 30 mg / g, ursolic acid 14.91 mg / g). Bidenoside C is 8.75 mg / g, and procyanidin is 15.64 mg / g, a significant amount of procyanidin A2. Both the effective amount of MF and the number of compounds decreased. It lays the foundation for the development of new hepatoprotective drugs with low dosage and easy quality control. The second part Cynomorium songaricium is a kind of chlorophyll free. The whole parasitic plant is mainly concentrated in the northwest of China. In this study, Chaoyang was used as the raw material to prepare the product of Chaoyang, which was crushed into pea-sized granules, and the triterpenoids were extracted by 95%% ethanol. Chaoyang triterpene chewable tablets were prepared from ethanol extract containing triterpenes, and the formula was determined as 10% microcrystalline cellulose. The formula was: 1. 30% triterpenoid 15% milk powder, 17% sugar powder, 27% corn starch and 10% microcrystalline cellulose. 0.22% glycyrrhizic acid 0.78% citric acid; After ethanol was extracted, the residue was extracted with cysteine plus vitamin C or water at 60 鈩，

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