加味黄芪建中汤对消化性溃疡的临床与实验研究

发布时间：2018-01-18 07:21

  本文关键词：加味黄芪建中汤对消化性溃疡的临床与实验研究　出处：《延边大学》2017年硕士论文　论文类型：学位论文

  更多相关文章： 加味黄芪建中汤 消化性溃疡 临床观察 胃蛋白酶 一氧化氮

【摘要】：研究目的:本临床疗效观察与动物实验的目的主要是为了探讨加味黄苗建中汤对顺化性溃疡治疗作用及其机理。研究方法:1.选择已经确诊为消化性溃疡的患者60名又必须都符合中医证型(脾胃虚寒证),60名患者是根据每位患者到医院诊疗的日期与时间顺序分为中药治疗组30名与西药对照组30名。中药治疗组用加味黄苗建中汤(黄芪20g,饴糖20g,桂枝10g,芍药15g,干姜5g,大枣5枚,炙甘草5g,白芨10g,小茴香10g)治疗,西药对照组采用奥美拉唑肠溶片(由江苏联环药业股份有限公司生产,批准文号:国药准字H10980267)治疗。本研究以治疗4周为一个疗程,治疗4周后比较分析两组的临床疗效,中医主要症状积分变化,出现的不良反应。最后观察所得数据利用SPSS22.0软件包进行统计分析。2.健康清洁级雄性SD小鼠,50只随机分为5组,依次为:正常对照组、胃溃疡模型组、西药对照组(奥美拉唑组)、加味黄苗建中汤高剂量组、加味黄芪建中汤低剂量组,一个组放10只,分组饲养。正常组除外的每组均采用乙酸灌胃制造小鼠胃溃疡模型。造模成功后24小时对所有小鼠恢复正常喂养;对西药对照组(奥美拉唑组)、加味黄芪建中汤高剂量组、加味黄芪建中汤低剂量组给予药物的治疗。持续治疗3周后,小鼠禁食不禁水24小时,采用摘除眼球采血的方法,采集的血液尽快离心后放置在-20℃的冰箱中保存。然后取出胃部,沿胃角切开胃壁,放入装有生理盐水的器皿中在温度为4℃的冰箱中冷藏保存。用肉眼观察胃溃疡的大体程度,检测胃蛋白酶活力、血清一氧化氮(NO)的含量。最后用SPSS22.0统计软件进行统计分析实验数据。研究结果:1.临床疗效观察结果:在临床疗效方面:中药治疗组总有效率为83.3%西药对照组总有效率为86.7%,所以中药治疗组与西药对照组的临床疗效无显著性差异(P0.05),中药治疗组与西药对照组临床疗效都很显著。中医临床症状积分变化方面:中药治疗组的中医症状积分降低程度与西药对照组的中医症状积分降低程度有显著性差异(P0.05),中药治疗组的中医症状积分降低程度更大。出现的不良反应:两组治疗期间出现的不良反应次数比较有显著性差异(P0.05),治疗组治疗期间不良反应出现次数为5例,对照组治疗期间不良反应出现次数为12例。2.动物实验结果:加味黄苗建中汤高剂量组与低剂量组和模型组比较时低剂量组的胃蛋白酶活力降低(P0.05),而高剂量组的胃蛋白酶活力显著降低(P0.01);测血清一氧化氮(NO)中高低剂量组与模型组比较时高剂量组的一氧化氮(NO)增加(P0.05),而低剂量组的一氧化氮(NO)显著增加(P0.01)。结论:临床疗效观察:(1)加味黄苗建中汤在改善中医症状积分方面优于单纯奥美拉唑的西药对照组。(2)加味黄苗建中汤在不良反应的出现方面优于单纯奥美拉唑的西药对照组。动物实验:(1)加味黄芪建中汤各剂量组能降低模型小鼠胃蛋白酶活力,高剂量组与低剂量组相比时高剂量组的疗效更佳。(2)加味黄芪建中汤各剂量组能增加模型小鼠血清一氧化氮含量,高剂量组与低剂量组相比时低剂量组的疗效更佳。
[Abstract]:Objective: To study the clinical and animal experiment is aimed to explore the effect of Jiawei Decoction on the treatment effect of Huang Miaojian in hue ulcer and its mechanism. Methods: 1. selection has been diagnosed as peptic ulcer and 60 patients with TCM syndrome type must (spleen and stomach deficiency cold syndrome), 60 patients according to the date the diagnosis and treatment of each patient to the hospital and the time sequence is divided into Chinese medicine treatment group 30 and control group of Western medicine. 30 traditional Chinese medicine treatment group with modified yellow Miaojian Decoction (Radix Astragali 20g, maltose 20g, cinnamon 10g, peony 15g, 5g dried ginger, jujube 5 pieces, fried licorice 5g, Bletilla 10g, cumin 10g) treatment the western medicine group, with Omeprazole Enteric-coated Tablets (by Jiangsu pharamceutical Limited by Share Ltd production approval number: Zhunzi H10980267). In this study, treatment for 4 weeks for a course of treatment, after 4 weeks of treatment, compared the clinical efficacy of the two groups, mainly Chinese Medicine The symptoms integral change, adverse reactions. The last observed data package statistical analysis.2. healthy male SD mice by using SPSS22.0 software, 50 rats were randomly divided into 5 groups, respectively: normal control group, gastric ulcer model group, western medicine control group (omeprazole group), modified Huang Miaojian Decoction high dose group modified Huangqijianzhong Decoction low dose group, a group of 10, group feeding. Each group except for normal group were intragastric administration by acetic acid producing gastric ulcer in mice model. After the modeling 24 hours for all the mice returned to normal feeding; the western medicine control group (omeprazole group), modified Huangqijianzhong Decoction high dose modified Huangqijianzhong Decoction group, low dose group received drug therapy. After 3 weeks of continuous treatment, mice were fasted for 24 hours, using the method of removal of the eye blood, the blood collected as soon as possible after centrifugation, stored in the refrigerator. Then the -20 C Remove the stomach, the stomach angle cut into the stomach, with physiological saline at the temperature of refrigerated containers in the refrigerator 4 degrees. In observing the degree of gastric ulcer with the naked eye, the detection of pepsin activity, serum nitric oxide (NO) content. Finally using SPSS22.0 statistical software for statistical analysis of experimental data. Results: 1. results: the clinical curative effect in clinical curative effect: treatment group total effective rate was 83.3% in the western medicine group the total effective rate was 86.7%, so there was no significant difference between the clinical curative effect of traditional Chinese medicine treatment group and Western medicine control group (P0.05), Chinese medicine group and Western medicine group clinical curative effect is very notable. TCM clinical symptom score changes: TCM symptoms of Chinese medicine treatment group to reduce the degree of the symptom integral of traditional Chinese medicine and Western medicine control group had significant difference (P0.05), reduce the degree of TCM symptoms of Chinese medicine treatment group to reduce the process A greater degree of adverse reaction. The number of adverse reactions: during the treatment of the two groups had significant difference (P0.05), the treatment group adverse reaction occurred during times of 5 cases, control group treatment adverse reactions occurred during the number of 12 cases of.2. animal experimental results: modified Huang Miaojian Decoction high dose group and low dose group and model group when compared with low dose group of pepsin activity decreased (P0.05), and high dose group of pepsin activity decreased significantly (P0.01); serum nitric oxide (NO) level of nitric oxide in the dose group compared with the model group, high dose group (NO) increased (P0.05), and low dose group nitric oxide (NO) increased significantly (P0.01). Conclusion: the clinical observation: (1) Huang Miaojian Jiawei Decoction in improving the TCM symptom score than omeprazole control group of Western medicine. (2) modified Huang Miaojian Decoction in the adverse reaction. The surface is better than that of omeprazole control group of Western medicine. Animal experiment: (1) modified Huangqijianzhong Decoction each dose group can decrease the activity of pepsin in mice, the curative effect is better in high dose group compared with the low dose group, high dose group. (2) modified Huangqijianzhong Decoction each dose group can increase the content of serum nitric oxide model the mice in high dose group, low dose group and the curative effect is better when compared with the low dose group.

【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R259
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本文编号：1440027