乌梅提取物预防大肠癌发生发展的实验研究

发布时间：2018-01-20 13:57

本文关键词： 乌梅提取物大肠癌炎癌转变化学预防　出处：《南京中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的：乌梅是常用的药食同源中药,本研究针对炎症性肠病癌变和大肠癌转移问题,探讨乌梅提取物预防肠道炎癌转化和迁徙转移的作用,证实乌梅提取物在大肠癌发生、发展中的防控作用。方法：1.分对照组,实验组,乌梅提取物组,实验组单纯运用AOM-DSS诱导的肠病癌变小鼠,乌梅提取物组给予药物干预,观察AOM-DSS诱导的肠病癌变小鼠体重、疾病活动指数(DAI)、小鼠结肠重量/长度比值的变化和小鼠肠道肿痛的形成情况；2.以乌梅提取物配成2 mg/ml溶液,对HT29人结肠肿瘤细胞进行干预,在24 h以后收集细胞,并用实时荧光定量PCR检测法,测定cox-2mRNA的表达,然后以划痕法检测细胞的迁移,观察乌梅提取物对TPA诱导的HT29细胞迁移的影响。结果：实验结果：1.通过对照组,实验组,乌梅提取物组三组比较,结果提示乌梅提取物能显著改善炎症性肠病癌变过程中模型小鼠体重下降的状况,改善肠病癌变小鼠不断进展的体质量下降、稀便及便血等症状,并能改善模型小鼠结直肠炎性水肿和异常增生,能够有效预防炎症性肠病向癌症转变。病理结果：正常组小鼠显微镜下固有层未见炎症,未见隐窝损伤；小鼠结肠壁粘膜上皮细胞未见不典型增生,未见肿瘤组织。模型组小鼠光镜下见重度炎症；结肠壁粘膜层查见肿瘤组织。而乌梅提取物组,所有小鼠未查见肿瘤。2.乌梅提取物能抑制TPA诱导的人结肠癌HT29细胞的迁移,显著下调人结肠癌HT29细胞中cox-2 mRNA的表达。结论：乌梅提取物能够有效预防AOM-DSS诱导的肠病癌变,具有防控肠道炎癌转化作用,并能够有效控制肠癌迁移转移,乌梅提取物对于大肠癌发生发展具有防控作用。
[Abstract]:Objective: black plum is a common medicine and food homologous Chinese medicine. This study aimed at the problem of carcinogenesis of inflammatory bowel disease and metastasis of colorectal cancer, and discussed the role of the extract of black plum in preventing the transformation and migration of intestinal inflammation and carcinoma. Methods the mice were divided into control group, experimental group, black plum extract group and AOM-DSS induced carcinogenesis mice. The mice with intestinal cancer induced by AOM-DSS were observed by drug intervention and disease activity index (DAI). The changes of colonic weight / length ratio and the formation of intestinal swelling and pain in mice; 2. Two mg/ml solution was prepared from the extract of Wupai, and the cells were collected after 24 hours of intervention in HT29 human colon tumor cells. The cells were detected by real-time fluorescence quantitative PCR. The expression of cox-2mRNA was measured and cell migration was detected by scratch method. Objective: to observe the effect of the extract of black plum on the migration of HT29 cells induced by TPA. Results: the experimental results: 1. The comparison of three groups: control group, experimental group and black plum extract group. The results suggested that the extract could significantly improve the weight loss of the model mice during the process of carcinogenesis of inflammatory bowel disease, and ameliorate the progressive decline in body mass, dilute stool and blood in stool of the mice with carcinogenesis of intestinal diseases. And can improve the model mice colitis edema and abnormal proliferation, can effectively prevent inflammatory bowel disease to cancer. Pathological results: normal group mice under microscope lamina propria no inflammation, no crypt damage; No atypical hyperplasia and tumor tissue were observed in colonic mucosal epithelial cells of mice. Severe inflammation was observed under light microscope in model group. Tumor tissue was found in the mucosal layer of colon wall, while in the extract group, no tumor was found in all mice. The extract could inhibit the migration of human colon cancer HT29 cells induced by TPA. The expression of cox-2 mRNA in human colon cancer HT29 cells was significantly down-regulated. Conclusion: the extract of Acanthus rubra can effectively prevent the carcinogenesis of intestinal diseases induced by AOM-DSS. It has the function of preventing and controlling the transformation of intestinal inflammation and cancer, and can effectively control the migration and metastasis of intestinal cancer.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R273

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