藏药复方多血康胶囊对脂多糖诱导急性肺损伤小鼠的保护作用

发布时间：2018-01-24 08:33

本文关键词： 藏药复方多血康胶囊急性肺损伤炎症氧化应激小鼠　出处：《中国药房》2017年07期 　论文类型：期刊论文

【摘要】：目的:考察藏药复方多血康胶囊对脂多糖诱导急性肺损伤小鼠的保护作用。方法:将60只KM小鼠随机分为空白组、模型组、地塞米松组(阳性对照,1 mg/kg)和复方多血康胶囊高、中、低剂量组(3.6、1.8、0.9 g/kg,以生药计),每组10只。ig给药,空白组和模型组小鼠ig等体积生理盐水,每天1次。连续给药7 d后,除空白组外,其余各组小鼠均ip脂多糖诱导急性肺损伤。造模6h后,观察小鼠肺组织病理改变,测量肺组织含水量,检测肺组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平以及血清中白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)水平。结果:与空白组比较,模型组小鼠肺组织发生充血水肿、炎症细胞浸润、肺泡间隔及肺泡壁明显增宽等病理变化,肺组织含水量和肺组织中MDA水平以及血清中IL-6、TNF-α水平均显著升高(P0.01),肺组织中SOD、GSH-Px水平显著降低(P0.05)。与模型组比较,各给药组小鼠肺组织损伤程度均不同程度降低;除复方多血康胶囊低剂量组大鼠肺组织含水量、肺组织中MDA水平以及血清中TNF-α水平变化不明显外,其余各给药组小鼠上述指标均显著改善(P0.05或P0.01)。结论:藏药复方多血康胶囊可明显减轻小鼠氧化应激和炎症反应,对脂多糖诱导的急性肺损伤小鼠有一定保护作用。
[Abstract]:Objective: to investigate the protective effect of Duoxuekang capsule on acute lung injury induced by lipopolysaccharide in mice. Methods: 60 km mice were randomly divided into blank group, model group and dexamethasone group (positive control group). 1 mg / kg) and compound Doxuekang capsule were given to 10 rats in each group. Mice in blank group and model group were given intravenously saline once a day. After 7 days of continuous administration, all the mice except blank group were induced by lipopolysaccharide (LPS) for acute lung injury. The model was established 6 hours later. The pathological changes of lung tissue, water content of lung tissue, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were observed. Results: compared with the blank group, the serum levels of malondialdehyde (MDA), interleukin-6 (IL-6) and tumor necrosis factor 伪 (TNF- 伪) in the lung tissue of the model group showed hyperemia and edema. Inflammatory cell infiltration, alveolar septum and alveolar wall obviously widened, lung water content, lung tissue MDA level and serum IL-6. The levels of TNF- 伪 increased significantly (P 0.01), and the levels of GSH-Px in lung tissue decreased significantly (P 0.05) compared with the model group. The degree of lung injury in each administration group was lower than that in the control group. The changes of water content in lung tissue, MDA level in lung tissue and serum TNF- 伪 level in low dose group of Fufang Duoxuekang capsule were not obvious. The above indexes in the other groups were significantly improved by P0.05or P0.01.Conclusion: Duoxuekang capsule can obviously alleviate the oxidative stress and inflammatory reaction in mice. It has protective effect on acute lung injury induced by lipopolysaccharide in mice.
【作者单位】：成都中医药大学民族医药学院;
【基金】：国家自然基金青年项目(No.8150141651) 成都中医药大学科技发展基金资助项目(No.030018002,CGPY1503) 医药行业科研专项(No.201507002)
【分类号】：R29
【正文快照】： 藏药复方多血康胶囊由红景天、沙棘、余甘子、干姜4味药材组成,为藏医大师措如·才郎的临床经验方,常用于治疗红细胞增多症。方中红景天养肺清热[1];沙棘除肺病,治培根病;余甘子治培根病、赤巴病、血病;干姜引肺脓、生胃火[2]。现代研究表明,红景天对高原肺动脉高压和大鼠急

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