丹参多酚酸盐干预非ST段抬高心肌梗死患者microRNA与靶基因

发布时间：2018-02-01 10:43

本文关键词： 非ST段抬高性心肌梗死微小RNA 丹参多酚酸盐治疗靶标生物信息学　出处：《中国实验方剂学杂志》2017年19期 　论文类型：期刊论文

【摘要】：目的:高通量检测非ST段抬高性心肌梗死(NSTEMI)患者药物干预前后的微小RNA(microRNA)表达谱,对比中西药物治疗前后microRNA表达变化,分析活血化瘀药物干预靶标与机制。方法:选取符合西医疾病诊断标准和中医证侯诊断标准的患者,分为2组,包括NSTEMI中药治疗组,NSTEMI西药组,每组纳入35例,抽取外周血,分离外周血单核细胞(PBMC),提取总RNA。根据各组差异表达的microRNA和基因的通路和网络分析结果,确定各组中起关键调控作用的microRNA及其靶基因。结果:在microRNA表达谱中,中药治疗组治疗前后、中药治疗组与西药组两组治疗后比较均存在差异;中药治疗组治疗后hsa-miR-92a,hsa-miR-363,hsa-miR-499,hsa-miR-30b,hsa-miR-454-3p,hsa-miR-933表达下调,hsa-miR-144,hsa-miR-451,hsa-miR-494,hsa-miR-320表达上调。表明这10个microRNA是丹参多酚酸盐干预NSTEMI的靶标。实时荧光定量PCR(Real-time PCR)验证,与西药组比较,中药治疗组治疗后hsa-miR-92a,hsa-miR-30b,hsa-miR-499,hsa-miR-454表达下调;中药治疗组治疗后hsa-miR-494的表达上调(P0.05)。结论:NSTEMI患者治疗前后,中药治疗组与西药组microRNA的表达谱均存在差异,筛选的miRNA可能是活血化瘀药物干预的靶标。
[Abstract]:Objective: to detect the microRNAs expression profile of non-ST-segment elevation myocardial infarction (NSTEMI) patients before and after drug intervention. Compare the changes of microRNA expression before and after western medicine treatment, analyze the intervention target and mechanism of drugs for promoting blood circulation and removing blood stasis. Methods: select the patients who accord with the diagnostic criteria of western medicine and TCM syndromes. The patients were divided into two groups, including NSTEMI treated group with NSTEMI western medicine group, each group included 35 cases. Peripheral blood was extracted and peripheral blood monocytes were isolated. Total RNA was extracted. The results of microRNA and gene network analysis were analyzed according to the differentially expressed microRNA and gene in each group. Results: in the microRNA expression profile, the traditional Chinese medicine treatment group before and after treatment. There were differences between the Chinese medicine treatment group and the western medicine group after treatment. In the treatment group, hsa-miR-92a, hsa-miR-363, hsa-miR-499and hsa-miR-30b were used. The expression of hsa-miR-454-3psa-miR-933 was down-regulated by hsa-miR-144, hsa-miR-451and hsa-miR-494. The expression of hsa-miR-320 was up-regulated, which indicated that these 10 microRNA were the targets of Salvia miltiorrhiza polyphenolic acid to interfere with NSTEMI. Real-time PCR) validation. Compared with the western medicine group, the expression of hsa-miR-92ahsa-miR-30bsa-miR-499nhsa-miR-454 was down-regulated in the traditional Chinese medicine treatment group. The expression of hsa-miR-494 was up-regulated in the Chinese medicine treatment group after treatment. Conclusion: before and after treatment, the expression of hsa-miR-494 in the treatment group is higher than that in the control group. There were differences in microRNA expression profiles between Chinese medicine group and western medicine group. The selected miRNA might be the target of blood circulation and blood stasis drug intervention.
【作者单位】：中国中医科学院广安门医院;
【基金】：国家自然科学基金项目(81473561)
【分类号】：R259
【正文快照】： [网络出版地址]http://kns.cnki.net/kcms/detail/11.3495.R.20170711.1401.046.html[网络出版时间]2017-07-11 14:01非ST段抬高性心肌梗死(NSTEMI)是一组可危及生命的重要临床综合征,常因冠状动脉粥样硬化斑块不稳定、斑块破裂导致冠状动脉内血栓形成、冠脉血流减少引起[1]。

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