UPLC-PDA同时测定藏药翼首草环烯醚萜苷和酚酸类10个化学成分的含量

发布时间：2018-02-08 16:30

  本文关键词： 藏药 翼首草 UPLC-PDA 环烯醚萜 酚酸 含量测定　出处：《中国中药杂志》2017年07期 　论文类型：期刊论文

【摘要】：建立超高效液相色谱法(UPLC-PDA)同时测定藏药翼首草环烯醚萜和酚酸类10个化学成分的含量。采用Acquity UPLC~R BEH C_(18)(2.1 mm×100 mm,1.7μm)色谱柱,流动相0.2%磷酸水(A)-乙腈(B),梯度洗脱,检测波长237,325 nm,流速0.4 mL·min~(-1),柱温30℃,进样量1μL。研究结果表明UPLC-PDA法定量分析翼首草10个化学成分在考察的范围内线性关系良好(r≥0.999 7);加样回收率为96.30%~103.0%(n=9),RSD为0.72%~2.9%(n=9)。所建立UPLC方法简便、准确、重复性好,可同时测定藏药翼首草中10个化学成分的含量。
[Abstract]:An ultra-high performance liquid chromatography (UPLC-PDA-HPLC) was developed for the simultaneous determination of 10 chemical constituents of Cyclopterpene and phenolic acids in Tibetan medicine. The chromatographic column was Acquity UPLC~R BEH Che 18 (2. 1 mm 脳 100 mm ~ 1. 7 渭 m). The mobile phase consisted of 0.2% phosphoric acid water, acetonitrile, acetonitrile, and gradient elution. The detection wavelength was 237,325 nm, the flow rate was 0.4 mL 路min ~ (-1) 路min ~ (-1), column temperature was 30 鈩，

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