朝医方“皂角大黄汤”对四氯化碳所致小鼠急性肝损伤的保护作用

发布时间：2018-02-13 05:17

本文关键词： 皂角大黄汤急性肝损伤 CCl_4 保护作用　出处：《延边大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的：探究皂角大黄汤对CCl。诱导的小鼠急性肝损伤的保护作用,并初步探究其可能的作用机制。方法：1.将正常饲养环境下饲养的雄性小鼠60只按完全随机分组法平分为6组,分别为正常对照组、阳性对照组、CCl4模型组。皂角大黄汤高、中、低剂量组,每组各10只小鼠。2.正常对照组与CCl。模型组每日予以相同体积的无菌生理盐水,阳性对照予以水飞蓟宾0.10 g/kg。皂角大黄汤高、中、低剂量组的给药量分别为：2.00/kg、1.00g/kg、0.50g/kg,给药体积为0.2ml/10g。给药方法每天灌胃一次,连续灌胃7天。3.实验开始造模采血之前,小鼠应24小时不禁水禁食。造模后摘除小鼠眼球采血,眼球取出来的血用离心机分离血清。采用颈部脱臼法处死小鼠。对处死的小鼠进行剖腹,取出大约0.1g肝组织,然后加入0.9mL生理盐水匀浆。4.最后测定血清中天门冬氨酸氨基转移酶(AST)活性、丙氨酸氨基转移酶(ALT)、总超氧化物歧化酶(T-SOD)的活性、谷肤甘肤过氧化物酶(GSH-Px)以及肝组织中丙二醛(MDA)的含量。结果：皂角大黄汤高、中、低剂量各组与CCl4模型组相比较,血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的含量明显降低;总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力明显增高,肝组织中丙二醛(MDA)含量明显降低。结论：本研究表明皂角大黄汤对CCl。诱导所致的小鼠急性肝损伤有保护作用。其机制可能与清除氧自由基,抑制脂质过氧化有关。
[Abstract]:Objective: to investigate the protective effect of Zaojiao rhubarb decoction on CCl. induced acute liver injury in mice and to explore its possible mechanism. Methods: 60 male mice raised in normal feeding environment were divided into 6 groups according to complete random grouping method. The rats were divided into normal control group, positive control group and CCl4 model group. 10 mice in each group were given the same volume of aseptic normal saline daily in the normal control group and CCl.Model group, 10 mice in each group with high, middle and low doses of rhubarb decoction, and the control group were given the same volume of aseptic saline daily. The positive control group was given silybin 0.10 g / kg. The dosage of high, middle and low dose groups was 0.50 g / kg, 0.50 g / kg, 0.2ml / 10 g / kg, respectively. The mice should be deprived of water for 24 hours. After the model was made, blood was collected from the eyeballs of the mice, and the blood from the eyeballs was separated by centrifuge. The mice were killed by cervical dislocations. The mice were cut off by laparotomy, and about 0.1 g of liver tissue was removed. Finally, the activity of aspartate aminotransferase (AST), alanine aminotransferase (alt) and total superoxide dismutase (T-SOD) were determined. The content of GSH-Px and malondialdehyde (MDA) in liver tissue. Results: compared with CCl4 model group, Zaojiao rhubarb decoction had high, middle and low dosage groups, and the content of GSH-Px and malondialdehyde (MDA) in liver tissue was higher than that in control group. The contents of alanine aminotransferase (alt), aspartate aminotransferase (AST) and total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) in serum were significantly decreased. Conclusion: this study shows that Zaojiao Dahuang decoction has protective effect on CCl. induced acute liver injury in mice. The mechanism may be related to scavenging oxygen free radicals and inhibiting lipid peroxidation.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R29

【相似文献】