电针不同时间介入对慢性脑缺血大鼠局灶性脑血流、认知功能及炎性损伤的影响

发布时间：2018-02-14 22:00

本文关键词： 电针慢性脑缺血局灶性脑血流认知功能炎性损伤　出处：《中国中西医结合杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的观察电针不同时间介入对慢性脑缺血(CCI)大鼠局灶性脑血流(r CBF)、认知功能及炎性损伤的作用。方法清洁级健康雄性SD大鼠37只,随机分为假手术组10只,手术组27只。手术组采用双侧颈总动脉结扎法(2-VO)法复制CCI模型,再随机分为模型组、电针4周组、电针1周组3个组,各组均选取8只,假手术组亦取8只。电针4周组电针干预4周,电针1周组最后1周进行电针干预。采用激光多普勒血流仪检测造模前、造模后、处死前r CBF,Morris水迷宫评定大鼠认知功能,ABC-ELISA法检测海马IL-6、TNF-α和IL-1β水平。结果与假手术组比较,手术组大鼠术后r CBF显著下降,且低于本组术前(P0.01)。与假手术组比较,模型组r CBF水平降低,目标象限停留时间减短,第2~5日逃避潜伏时间明显增长,IL-6、TNF-α和IL-1β水平增高(P0.01)。与模型组比较,电针4周组r CBF水平升高,目标象限停留时间增长,第3~5日逃避潜伏时间缩短,IL-6水平增加,IL-1β水平下降(P0.05,P0.01);电针1周组第5日逃避潜伏时间缩短(P0.05)。与电针1周组比较,电针4周组目标象限停留时间增长,第5日逃避潜伏时间缩短(P0.05)。结论电针能改善CCI大鼠r CBF和认知功能,减轻炎性损伤,早期干预作用更显著。
[Abstract]:Objective to observe the effects of electroacupuncture intervention on focal cerebral blood flow (CBFN), cognitive function and inflammatory injury in CCI rats at different times. Methods Thirty-seven healthy male SD rats of clean grade were randomly divided into sham operation group (n = 10) and sham operation group (n = 10). The CCI model was established by bilateral common carotid artery ligation in the operation group (n = 27). The model group was randomly divided into three groups: electroacupuncture group (n = 4), electroacupuncture group (n = 3), electroacupuncture group (n = 3), and each group (n = 8). Eight rats were taken from the sham operation group. Electroacupuncture intervention was performed in the 4 week group and the last week in the 1 week group. Laser Doppler flow meter was used to detect the model before and after modeling. The levels of IL-6 TNF- 伪 and IL-1 尾 in hippocampus were detected by ABC-ELISA before death. Results compared with sham operation group, r CBF in operation group decreased significantly and was lower than that in preoperation group (P 0.01). R CBF level decreased, target quadrant residence time decreased in model group, and escape latency time increased significantly from 2nd to 5th. Compared with model group, r CBF level increased and target quadrant residence time increased in electroacupuncture 4 weeks group compared with model group, and IL-6 TNF- 伪 and IL-1 尾 increased in model group. From the 3rd to 5th, the escape latency time shortened and the IL-6 level increased. The level of IL-1 尾 decreased. In the 1st week group, the escape latency time was shortened in 5th (P 0.05). Compared with the electroacupuncture group, the target quadrant retention time of the 4-week electroacupuncture group increased. Conclusion Electro-acupuncture can improve r CBF and cognitive function of CCI rats and alleviate inflammatory injury, and the early intervention effect is more obvious.
【作者单位】：浙江中医药大学附属第三医院针灸科;浙江中医药大学第三临床医学院针灸研究室;
【基金】：国家自然科学基金资助项目(No.81503645) 浙江省重点科技创新团队计划资助项目(No.2013TD15)
【分类号】：R245

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