细胞凋亡参与电针对退变椎间盘保护作用的研究

发布时间：2018-02-15 11:50

本文关键词： 电针夹脊穴腰椎间盘退变细胞凋亡　出处：《时珍国医国药》2017年09期 　论文类型：期刊论文

【摘要】：目的通过研究夹脊电针对兔退变的腰椎间盘中细胞凋亡调控基因Bax和Bcl-2表达的影响,探讨细胞凋亡参与电针治疗腰椎间盘退行性病变的作用机制。方法选用40只清洁级成年新西兰大白兔随机分为正常组、假模型组、模型组和治疗组,每组10只,组内又分为28d、56d两个取材时间点。造采用克式针横穿椎体后椎体间轴向加压的造模方法;假模型组只穿针,不予椎体间加压;治疗组针刺L4、L5夹脊穴28 d,各组于不同时间点取出椎间盘组织,应用Western Blot方法检测各组Bax和Bcl-2蛋白表达。结果 Western Blot检测发现正常组不同时间点之间Bax和Bcl-2蛋白表达差异无统计学意义,建立模型后,促凋亡蛋白Bax表达增强,而Bcl-2表达较正常组及家模型组均减弱,电针干预的方法可降低退变的兔椎间盘组织中Bax的表达,促进Bcl-2蛋白的合成(P0.05)。结论电针夹脊穴可以通过降低Bax和增加Bcl-2的表达,从而抑制细胞凋亡的发生或者清除细胞凋亡产物,达到防治椎间盘退变的目的。
[Abstract]:Objective to study the effect of spinal electroacupuncture on the expression of apoptosis-regulating genes Bax and Bcl-2 in rabbit lumbar intervertebral disc. To explore the mechanism of apoptosis in the treatment of degenerative lumbar intervertebral disc by electroacupuncture, 40 adult New Zealand rabbits were randomly divided into normal group, sham model group, model group and treatment group with 10 rabbits in each group. The rats in the group were divided into two time points of 28 days and 56 days. The model of axial compression between the vertebrae after crossing the vertebral body with the gram needle was made, the pseudo-model group was only punctured, and the intervertebral compression was not allowed in the sham model group. In the treatment group, the intervertebral disc tissue was removed at different time points at 28 days after acupuncture at Jiaji point L4 and L5. Results the expression of Bax and Bcl-2 protein in normal group was not significantly different from that in normal group at different time points by Western Blot method. The expression of apoptotic protein Bax was enhanced after the establishment of the model. The expression of Bcl-2 was lower than that of normal group and model group. Electroacupuncture intervention could decrease the expression of Bax and promote the synthesis of Bcl-2 protein in the degenerative intervertebral disc tissue of rabbits. Conclusion Electro-acupuncture at Jiaji point can decrease the expression of Bax and increase the expression of Bcl-2. In order to inhibit the occurrence of apoptosis or eliminate apoptosis products, to achieve the purpose of prevention and treatment of disc degeneration.
【作者单位】：武汉市第一医院;
【基金】：国家自然科学基金面上项目(No.81173324) 湖北省自然科学基金(No.2010CD034)
【分类号】：R245

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