损伤血瘀证大鼠血清TNF-α、β及骨骼肌细胞凋亡的变化研究

发布时间：2018-02-16 03:43

  本文关键词： 损伤血瘀证 凋亡 TNF-α TNF-β　出处：《成都体育学院》2016年硕士论文　论文类型：学位论文

【摘要】：目的:课题从损伤大鼠血清TNF-α与TNF-β以及骨骼肌细胞凋亡变化入手,观察TNF-α与TNF-β以及骨骼肌细胞凋亡在不同时相的变化规律,从而分析TNF-α、TNF-β、骨骼肌细胞凋亡变化的特征,部分揭示损伤血瘀证骨骼肌细胞的病理过程。方法:80只Wistar大鼠SPF级别,体重200-250g,每组雌雄各半随机分为8组。随机设立对照组C组(n=10只,下同),伤后1小时组(1H组),伤后12小时组(12H组),伤后1天组(1D组),伤后3天组(3D组),伤后5天组(5D组),伤后7天组(7D组),伤后14天组(14D组),采用“定量化重力击打法”[1]造成大鼠左大腿内群肌肉损伤,并选取大鼠血清以及左大腿内群肌肉,用ELISA法测定各组TNF-α与TNF-β的变化。用TUNEL法测定各组的骨骼肌细胞凋亡情况。应用SPSS17.0统计软件进行相关的统计方法分析。结果:1.在骨骼肌细胞凋亡变化方面,1D组、3D组、5D组、7D组、14D组比C组有极显著升高(P0.01)。3D组、5D组、7D组、14D组比1H组有极显著升高(P0.01),1D组比1H组有显著升高(P0.05)。3D组、5D组、7D组、14D组比12H组有极显著升高(P0.01),1D组比12H组有显著升高(P0.05)。1D组比C组有极显著升高(P0.01),比1H组、12H组有显著升高(P0.05)。7D组比C组、1H组、12H组有极显著升高(P0.01),比1D组有显著升高(P0.05)。2.在TNF-α数量变化方面,12H组比C组有极显著升高(P0.01),1H组比C组有显著升高(P0.05)。1H组比C组、14D组有显著升高(P0.05)。12H组比C组、14D组有极显著升高(P0.01),12H组比1D组、5D组、7D组TNF-α数量有显著升高(P0.05)。3.在TNF-β数量变化方面,12H组、1D组比C组有极显著升高(P0.01),1H组比C组有显著升高(P0.05)。4.TNF-α与TNF-β在本次研究中相关性低(P≥0.05);TNF-α与TNF-β的比值与反比值无明显统计学差异(P0.05)。结论:1.损伤血瘀证病理过程中骨骼肌细胞凋亡的变化在损伤后24小时最为显著。2.损伤血瘀证中大鼠血清TNF-α的波动变化主要发生在损伤后24小时之内,损伤后12小时TNF-α的测试值达到高峰;可能提示TNF-α是损伤血瘀证骨骼肌细胞凋亡发生的诱导因素之一。3.损伤血瘀证中大鼠血清TNF-β的上升波动变化主要发生在24小时之内;这可能也是激发损伤血瘀证骨骼肌细胞凋亡的重要因素。4.TNF-α与TNF-β之间未呈现线性相关关系,其比值与反比值在损伤过程中的不同时间点未产生显著的变化,即保持相对稳态,说明不同时间点TNF-α与TNF-β的偏移并没有打破二者之间的比值平衡关系,这有可能类似于中医所说的阴阳平衡关系,因此阴性结论也有进一步科学探索的价值。
[Abstract]:Objective: to observe the changes of TNF- 伪 and TNF- 尾 and skeletal muscle cell apoptosis in different phases from the changes of serum TNF- 伪 and TNF- 尾 and skeletal muscle cell apoptosis in injured rats, so as to analyze the characteristics of TNF- 伪 TNF- 尾 and skeletal muscle cell apoptosis. Methods 80 Wistar rats, weighing 200-250 g, were randomly divided into 8 groups. 1 hour after injury, 1H group, 12 hours after injury, 12 hours after injury, 1 day after injury, 1 day after injury, 3 days after injury, 5 days after injury, 5 days after injury, 5 days after injury, 7 days after injury, 7 days after injury, 7 days after injury, 14 days after injury, 14 days after injury, 14 days after injury, 14 days after injury, 14 days after injury. The method [1] caused muscle damage in the left thigh of rats. Serum and left thigh muscles were selected. The changes of TNF- 伪 and TNF- 尾 in each group were measured by ELISA method. Apoptosis of skeletal muscle cells in each group was determined by TUNEL method. The correlation statistical analysis was carried out by SPSS17.0 statistical software. Results 1. The P0.01D group was significantly higher than that of the 1H group, the P0.05D group was significantly higher than the 1H group, the P0.05D group was significantly higher than that of the 1H group, and the P0.01D group was significantly higher than that of the 12H group. There was a very significant increase in the P0.01D group compared with the 12H group in the 7D group and the 14D group in the 7D group compared with the 12H group. There was a very significant increase in the P0.01D group compared with the 12H group in P0.05D group. 1D group in the 1D group was significantly higher than that in the 12H group, and the value of P0.05D group was significantly higher than that in the 12H group. Compared with group C, group C had a very significant increase in P0.01D, and group 12D in group 1H was significantly higher than group C in P0.05, group 7D, group C, group C, group 1H, group 12H, and group C, group C, the level of P0.05A was significantly higher than that in group C, P0.05n.2.The quantity of TNF- 伪 in 12H group was significantly higher than that in group C, and that in group 12H was significantly higher than that in group C. There was a significant increase in the number of TNF- 伪 in group P0.05U 路1H than in group C (P 0.05U. 12H) than in group C (group C) 14D. The amount of TNF- 伪 in group 7D was significantly higher than that in group C (P 0.01D), and the change of TNF- 尾 in group 12H was significantly higher than that in group C (P 0.01 ~ 1H), and the level of TNF- 伪 in group 12H was significantly higher than that in group C (P < 0.05), and the level of TNF- 伪 in group C was significantly higher than that in group C (P < 0.05), and the level of TNF- 伪 in group C was significantly higher than that in group C. The correlation between TNF- 伪 and TNF- 尾 was significantly higher than that in group C (P 鈮，

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