电针完骨穴对血管性痴呆大鼠学习记忆能力的影响

发布时间：2018-03-03 10:56

本文选题：血管性痴呆　切入点：海马　出处：《锦州医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的探讨电针完骨穴(其位置类似于小脑顶核),对血管性痴呆大鼠学习与记忆能力的影响,对海马神经细胞的形态,以及对海马炎性细胞因子m RNA表达情况的影响,为血管性痴呆的治疗探索新的方案。方法选取健康雄性SD大鼠54只,每只大鼠的体重在300-450g,将其随机平分为三组:假手术组、血管性痴呆组、电针组,采用一侧大脑中动脉闭塞法建立血管性痴呆大鼠模型,通过连续进行5d的水迷宫测试,选取成功建立血管性痴呆模型的大鼠。电针组大鼠每天进行一次完骨穴电针刺激,电针参数为疏波30Hz,密波100Hz,强度6-15V,每次20min,持续4个月。应用水迷宫行为学实验对各组大鼠进行学习能力、记忆能力的测定,采用HE染色法观察海马神经细胞变化,同时采用Real-time PCR法检测大鼠海马炎性细胞因子(IL-1β、IL-6和TNF-α)m RNA的表达情况。结果1.血管性痴呆组大鼠平均逃避潜伏期为(37.01±7.69)s;电针组大鼠平均逃避潜伏期为(28.19±7.18)s。与血管性痴呆组相比,电针组大鼠平均逃避潜伏期明显缩短(P0.05)。2.与血管性痴呆组大鼠相比,电针组大鼠海马神经细胞损伤程度下降,电针完骨穴可以使大鼠海马神经细胞在数量上得以提升、在形态上得以改善。3.与血管性痴呆组大鼠相比,电针组大鼠IL-1β、IL-6和TNF-αm RNA的表达显著下降(第2月P0.05,第4月P0.01)。结论本实验初步证实了电针完骨穴可以使血管性痴呆大鼠的学习能力及记忆能力得到提升,同时电针可以减轻海马神经细胞的损伤程度,同时可以降低海马炎性细胞因子m RNA的表达水平,为血管性痴呆的诊治提供了新的方案。
[Abstract]:Objective to investigate the effect of electroacupuncture on the learning and memory ability of vascular dementia rats, the morphology of hippocampal nerve cells and the expression of inflammatory cytokine m RNA in hippocampus. Methods 54 healthy male SD rats with body weight of 300-450 g were randomly divided into three groups: sham operation group, vascular dementia group and electroacupuncture group. The rat model of vascular dementia was established by unilateral middle cerebral artery occlusion. The rats with vascular dementia were successfully established by water maze test for 5 days. The parameters of electroacupuncture were sparsely wave 30 Hz, dense wave 100 Hz, intensity 6-15 V for 20 min each time for 4 months. The learning ability and memory ability of rats in each group were measured by water maze experiment, and the changes of hippocampal nerve cells were observed by HE staining. Real-time PCR method was used to detect the expression of IL-1 尾 and TNF- 伪 RNA in hippocampus of rats. Results 1. The average escape latency of vascular dementia group was 37.01 卤7.69 s, and the average escape latency of electroacupuncture group was 28.19 卤7.18 s. Compared with vascular dementia group, the average escape latency of vascular dementia group was higher than that of vascular dementia group. Compared with the rats of vascular dementia group, the injury degree of hippocampal nerve cells in electroacupuncture group was decreased, and the number of hippocampal nerve cells in rats was increased by electroacupuncture at Wangu point. Compared with vascular dementia rats, The expression of IL-1 尾 -IL-6 and TNF- 伪 m RNA decreased significantly in the electroacupuncture group (P0.05 on February and P0.01A on April. Conclusion this experiment preliminarily proves that the learning ability and memory ability of vascular dementia rats can be improved by electroacupuncture at Wanku point. At the same time, electroacupuncture can reduce the degree of injury of hippocampal nerve cells and decrease the expression of inflammatory cytokine m RNA in hippocampus, which provides a new scheme for the diagnosis and treatment of vascular dementia.
【学位授予单位】：锦州医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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