按摩联合针刺疗法对大鼠运动型骨骼肌损伤的保护作用

发布时间：2018-03-11 22:19

本文选题：运动型骨骼肌损伤　切入点：针刺　出处：《中国老年学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨按摩联合针刺疗法对大鼠运动型骨骼肌损伤肱三头肌超微结构及磷酸肌酸激酶的影响及对运动型骨骼肌损伤的保护作用。方法将130只SD大鼠随机分为非运动组(安静对照组)和运动组,运动组按照干预方式不同分为运动对照组、按摩组、针刺组、联合组,每一运动组又按照样品采集时间不同分为以下亚组,包括即刻组、24 h组、48 h组。共计13组,每组10只大鼠。运动组在大鼠跑台上进行持续性下坡跑,做力竭离心运动。力竭运动后对照组不做任何处理,按摩组每日二次给予肱三头肌的揉捏推压和牵引按摩5 min。针刺组给予每日一次毫针斜刺肱三头肌,留针5 min。联合组在给予按摩治疗同时给予针刺疗法。实验方案完成后在运动后即刻、24 h、48 h取大鼠血清行磷酸肌酸激酶检测。取肱三头肌组织在镜下观察超微结构的变化,主要为细胞结构的异常与Z线的异常。结果力竭离心运动后大鼠肱三头肌超微结构发生改变,包括细胞结构异常(肌纤维排列紊乱,细胞膜模糊、溶解,线粒体肿胀、空泡样改变)和Z线异常(包括Z线流、Z线模糊、Z线扭曲和Z线消失)。这些超微结构异常改变在运动后24 h最明显,48 h有所恢复。而磷酸肌酸激酶在运动后即刻开始显著升高,48 h有所恢复,但仍高于安静对照组。各治疗组超微结构损害在24 h和48 h均较运动对照组减轻,联合治疗组损伤最轻;磷酸肌酸激酶值在24 h和48 h较运动对照组降低。联合治疗组磷酸肌酸激酶值最低。结论 (1)力竭离心运动可引起骨骼肌超微结构损害和磷酸肌酸激酶升高。(2)磷酸肌酸激酶更能早期反映骨骼肌的损伤。(3)按摩联合针刺疗法可以减轻骨骼肌超微结构的损害和降低磷酸肌酸激酶,说明两者联合对运动型骨骼肌损伤具有保护作用。
[Abstract]:Objective to investigate the effects of massage combined with acupuncture therapy on the ultrastructure of triceps brachii muscle and the protective effect of phosphocreatine kinase on exercise skeletal muscle injury in rats. Methods 130 Sprague-Dawley rats were randomly divided into two groups. Exercise group (quiet control group) and exercise group, The exercise group was divided into exercise control group, massage group, acupuncture group and combined group according to the intervention methods. Each exercise group was divided into the following subgroups according to sample collection time, including immediate group, 24 h group, 48 h group, total 13 groups. There were 10 rats in each group. The exercise group performed continuous downhill running and exhaustive centrifugation on the platform of the rats. The control group did not do any treatment after exhaustive exercise. The massage group was given the kneading and pressing of triceps brachii twice a day and the traction massage for 5 mins, while the acupuncture group was given the triceps brachii muscle once a day by oblique needling. The rats in the combined group were treated with massage and acupuncture at the same time. The serum samples of rats were taken immediately after exercise for 24 hours and 48 hours after exercise to detect creatine phosphokinase. The changes of ultrastructure of triceps brachii muscle tissue were observed under microscope. Results after exhaustive centrifugation, the ultrastructure of triceps brachii muscle changed, including abnormal cell structure (disordered arrangement of muscle fibers, blurred cell membrane, dissolution of mitochondria, swelling of mitochondria). Vacuolar changes) and Z-line abnormalities (including Z-line flow, fuzzy Z-line distortion and Z-line disappearance). These ultrastructural changes were most pronounced at 24 h after exercise and recovered at 48 h after exercise, while creatine phosphokinase recovered immediately after exercise. It began to increase significantly at 48 h, and recovered after 48 h. The ultrastructural damage in each treatment group was lighter than that in the exercise control group at 24 h and 48 h, and the damage in the combined treatment group was the least. The value of phosphocreatine kinase at 24 h and 48 h was lower than that in the exercise control group, and the value of phosphocreatine kinase was the lowest in the combined treatment group. Conclusion the exhaustive centrifugal exercise can cause ultrastructural damage of skeletal muscle and increase of creatine phosphokinase. Creatine kinase can reflect skeletal muscle injury in early stage. Massage combined with acupuncture therapy can reduce the damage of skeletal muscle ultrastructure and reduce creatine phosphokinase. These results suggest that the combined therapy has protective effect on the injury of sports skeletal muscle.
【作者单位】：南京审计大学体育教学部;南京师范大学体育科学学院体育教学部运动训练系;
【分类号】：R245

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