云南春燥环境对小鼠津液影响的相关实验研究

发布时间：2018-03-17 18:44

本文选题：燥证　切入点：云南春燥环境　出处：《云南中医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:通过观察云南春燥环境对小鼠生物表征和体内津液的影响,以探讨云南春燥的内在本质,揭示其证候的微观机制,并为建立云南春燥实验动物模型提供思路。方法:以中医病因学说为指导,将实验小鼠随机分为正常对照组,气候组,食物组和气候食物组,利用人工气候箱及香燥食物模拟云南春燥环境并作用于各组实验小鼠,观察各组小鼠的一般生物表征的变化,采用HE染色法观察各组小鼠皮肤组织,肺组织,大肠组织的病理形态学改变,记录各组小鼠皮肤组织,肺组织及大肠组织的干湿重计算其各组织的含水率的变化。结果:与正常对照组小鼠相比,气候组小鼠从施加因素第3天起出现打斗现象,尾部可见伤痕,并逐渐表现出烦躁易怒的状态,毛发逐渐稀疏,缺少光泽,爪甲颜色暗紫;食物组小鼠在施加因素后可见粪便便质略显干燥;气候食物组小鼠在施加因素后皮毛逐渐稀疏,易脱落,缺乏光泽,前期偶有打斗现象,尾部可见伤痕,后期趋于平静,爪甲颜色正常。与正常对照组小鼠第7天相比,气候组小鼠第7天体重降低(P0.05),肺组织出现病理形态学改变,肺组织含水率下降(P0.01),大肠组织含水率下降(P0.01);食物组小鼠第7天可见肺组织及大肠组织病理形态学改变,肺组织含水率下降(P0.01),大肠组织含水率下降(P0.01);气候食物组小鼠第7天体重下降(P0.01),肺组织出现病理形态学改变,肺组织含水率下降(P0.01),大肠组织含水率下降(P0.01)。与正常对照组小鼠第14天相比,气候组小鼠第14天体重降低(P0.01),肺组织出现病理形态学改变,肺组织含水率下降(P0.01),大肠组织含水率下降(P0.05),小鼠14天的饮水量增加(P0.01),小便量减少(P0.01);食物组小鼠第14天肺组织含水率下降(P0.05),大肠组织含水率下降(P0.01),小鼠14天的饮水量增加(P0.05),小便量减少(P0.01);气候食物组小鼠第14天可见肺组织病理形态学改变,肺组织含水率下降(P0.01),小鼠14天的饮水量增加(P0.01),小便量减少(P0.05)。结论:1.云南地区独特的地域环境可致使小鼠出现干燥的生物表征变化,且随着施加气候因素时间的延长,这种变化越来越明显,而随着施加饮食因素时间的延长,这种变化逐渐减弱。2.云南春燥环境中的气候因素可影响小鼠肺组织病理形态学的改变,且能够减少肺组织和大肠组织的含水率。3.云南春燥环境中的食物因素在实验初期可影响小鼠肺组织和大肠组织病理形态学的改变,且能够减少肺组织和大肠组织的含水率。4.云南春燥环境中的气候因素和饮食因素均能对小鼠体内津液的整个生成、输布和代谢过程产生影响,且肺与大肠津液下降到一定程度时,会影响两者出现病理形态学的改变。
[Abstract]:Objective: to explore the intrinsic nature of Yunnan spring dryness and reveal the microscopic mechanism of its syndromes by observing the effect of Yunnan spring dryness environment on biological characterization and body fluid in mice. Methods: the experimental mice were randomly divided into normal control group, climate group, food group and climate food group under the guidance of the theory of etiology of traditional Chinese medicine. The artificial climate box and fragrant dry food were used to simulate Yunnan spring dryness environment and acted on experimental mice in each group. The changes of general biological characteristics of each group were observed, and the skin and lung tissues of each group were observed by HE staining method. The pathomorphological changes of large intestine tissue were recorded, and the changes of moisture content in the skin, lung and large intestine tissue were calculated. Results: compared with the normal control group, the changes of moisture content in each tissue of each group were compared with those of the normal control group. From the third day after the application of the factors, the climate group mice appeared the phenomenon of fighting, the tail visible scars, and gradually showed irritable and irritable state, hair gradually sparse, lack of luster, claw color dark purple; In the food group, the fecal stool was slightly dry after applying the factors, and the skin of the climate food group was gradually sparse, easy to fall off, lacking of gloss, occasional fighting in the early stage, visible scars in the tail, and the later period tended to be calm, after the application of these factors, the mice in the food group showed a slight dry fecal feces. The color of claw armour was normal. Compared with the control group mice on the 7th day, the mice in the climate group lost their body weight on the 7th day, and the lung tissue showed pathomorphologic changes. The decrease of water content in lung tissue (P0.01A) and the decrease of water content in large intestine (P0.01C) were observed in mice of food group on the 7th day, the histopathological changes of lung tissue and large intestine tissue were observed in the food group. The contents of water in lung tissue and large intestine were decreased (P 0.01), and the weight of mice in climate food group was decreased on day 7 (P 0.01), and the pathological morphology of lung tissue was observed. Compared with the control group on the 14th day, compared with the control group, the mice in the climate group lost their body weight on the 14th day, and the pathological morphology of the lung tissue was observed, compared with the control group on the 14th day, compared with the control group on the 14th day, compared with the control group, the mice in the climate group lost their body weight on the 14th day. The water content of lung tissue decreased P0.01A, the water content of large intestine tissue decreased P0.05N, the drinking water of mice increased P0.01D and the amount of urine decreased P0.01G on the 14th day of food group, the water content of lung tissue decreased P0.05N on the 14th day of food group, the water content of large intestine tissue decreased P0.01, and the drinking water of mice was drinking water for 14 days. The increase of P0.05, the decrease of urine volume and the change of pathomorphology of lung tissue were observed on the 14th day in the climate food group. The decrease of water content in lung tissue (P0.01A), the increase of drinking water in mice (14 days) and the decrease of urine volume (P0.05). Conclusion: 1. The special regional environment in Yunnan region can cause dry biological characterization changes in mice, and with the extension of the time of applying climatic factors, This change became more and more obvious, but with the prolongation of the time of applying dietary factors, the change gradually weakened .2.Climatic factors in spring dryness environment in Yunnan could affect the pathological changes of lung tissue in mice. Food factors in spring dryness environment in Yunnan could influence the pathomorphological changes of lung and large intestine tissues in mice at the beginning of the experiment. And it can reduce the moisture content of lung tissue and large intestine tissue. 4. The climatic factors and diet factors in spring dryness environment in Yunnan can affect the whole production, distribution and metabolism of body fluid in mice. When lung and large intestine fluid decreased to a certain extent, the pathomorphologic changes were affected.
【学位授予单位】：云南中医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R-332;R228

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