补肾生血法治疗慢性髓劳病的临床疗效及其对Kv1.3mRNA的影响

发布时间：2018-03-17 22:15

本文选题：髓劳病　切入点：贫血　出处：《黑龙江中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:采用补肾生血法对30例慢性髓劳病患者治疗6个月,通过外周血象、中医证候积分等数据的疗前、疗后对比,观察补肾生血法治疗慢性髓劳病的临床疗效及疗前疗后骨髓中Kv1.3mRNA表达水平的差异性变化,并与10位志愿者组成的健康对照组进行对比,以了解慢性髓劳病患者Kv1.3mRNA表达水平与正常人之间的差异性。探讨Kv1.3mRNA表达水平变化与慢性髓劳病发病机制之间可能存在的关系,从而推测补肾生血法治疗慢性髓劳病可能存在的的疗效机理。方法:①选择30位已确诊的慢性髓劳病患者为治疗组,采用补肾生血法对这些患者进行为期6个月的治疗,观察并记录这些患者的临床疗效、中医证候积分及外周血象等在治疗前后的变化;②采集这些患者用补肾生血法治疗前后的骨髓,采用RT-PCR法检测骨髓液中Kv1.3mRNA表达水平治疗前后的变化。结果:①患者经补肾生血法治疗的临床有效率为80.0%,患者经补肾生血法治疗六个月后症状均有明显好转,中医证候积分较疗前也显著降低,差异有统计学意义(P0.05);②治疗后患者外周血血象各项指标明显升高,差异有统计学意义(P0.05);③患者骨髓中Kv1.3mRNA表达水平在经过补肾生血法治疗六个月后,表达水平明显上升,但仍低于健康对照组骨髓中Kv1.3mRNA的表达水平,差异均有统计学意义(P0.05)。结论:①补肾生血法对于慢性髓劳病的临床治疗具有肯定意义,无论在降低中医证候积分、改善中医证候、还是提高外周血象等方面都取得了显著的疗效。②慢性髓劳患者Kv1.3mRNA表达水平的低表达状态可能与慢性髓劳的病机存在某种关系。③补肾生血法可能通过调节Kv1.3mRNA表达水平而调节整合素介导的造血微环境损伤,从而提高骨髓造血能力,促进造血干/祖细胞的增殖分化,最终达到治疗慢性髓劳病的目的。
[Abstract]:Objective: to treat 30 patients with chronic pulpitis for 6 months by tonifying the kidney and generating blood, and to compare the data before and after treatment with the data of peripheral blood picture and TCM syndromes integral, etc. To observe the clinical effect of tonifying kidney and blood generating method in treating chronic myellabour disease and the difference of Kv1.3mRNA expression in bone marrow after treatment, and to compare it with the healthy control group composed of 10 volunteers. In order to understand the difference between the expression of Kv1.3mRNA in patients with chronic myellabour disease and normal subjects, and to explore the possible relationship between the changes of Kv1.3mRNA expression and the pathogenesis of chronic myellabour disease. Thus, the possible therapeutic mechanism of tonifying the kidney and generating blood was speculated. Methods 30 patients with chronic myellabour disease were selected as the treatment group and treated for 6 months with the method of tonifying kidney and generating blood. Observe and record the clinical curative effect of these patients, TCM syndromes integral and peripheral blood image changes before and after treatment to collect the bone marrow of these patients before and after treatment with the method of tonifying kidney and generating blood. RT-PCR method was used to detect the changes of Kv1.3mRNA expression in bone marrow fluid before and after treatment. Results the clinical effective rate of Bushen Shengxue therapy was 80.00.The symptoms of the patients treated with Bushen Shengxue method for six months were obviously improved. The score of TCM syndromes was also significantly lower than that before treatment. The difference was statistically significant (P 0.05). After treatment, the indexes of peripheral blood were significantly increased, and the level of Kv1.3mRNA expression in bone marrow of patients with P0.05 + 3 was significantly increased after six months of treatment with Bushen and Xue. The expression level of Kv1.3mRNA in bone marrow of healthy control group was significantly higher than that of healthy control group (P 0.05). Conclusion the method of tonifying kidney and generating blood by 1% 1 has positive significance for the clinical treatment of chronic Myelopathy, regardless of decreasing the integral of TCM syndromes. Improve TCM syndromes, The low expression level of Kv1.3mRNA in patients with chronic myelosis may have some relationship with the pathogenesis of chronic myelosis. 3 the method of tonifying kidney and generating blood may regulate the water expression of Kv1.3mRNA. Mediating integrin mediated hematopoietic microenvironment injury, Thus, the hematopoiesis ability of bone marrow was improved, and the proliferation and differentiation of hematopoietic stem / progenitor cells were promoted.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R259

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