苗药透骨香的质量标准研究

发布时间：2018-03-18 16:49

  本文选题：透骨香　切入点：薄层色谱法　出处：《中国药房》2017年33期 　论文类型：期刊论文

【摘要】：目的:建立透骨香药材的质量标准。方法:采用薄层色谱法(TLC)对药材进行定性鉴别;测定药材水分、总灰分和酸不溶性灰分;采用高效液相色谱法测定药材中水杨酸甲酯的含量:色谱柱为Eclipse XDB-C18,流动相为甲醇-水(62∶38,V/V),流速为1.0 mL/min,检测波长为307 nm,柱温为30℃,进样量为10μL。结果:药材的TLC图斑点清晰,分离度好。药材水分为8.2%~10.8%,总灰分为0.9%~4.0%,酸不溶性灰分为0.1%~0.9%。水杨酸甲酯检测进样量线性范围为0.045~0.73μg(r=0.999 9);精密度、稳定性、重复性试验的RSD≤1.0%;水杨酸甲酯加样回收率为97.8%~104.3%(RSD=2.6%,n=9)。结论:该研究所建标准可用于透骨香药材的质量控制。
[Abstract]:Objective: to establish the quality standard of TLC. Methods: TLC was used to identify the medicinal materials qualitatively, to determine the moisture, total ash and acid insoluble ash. The content of methyl salicylate in medicinal materials was determined by HPLC. The chromatographic column was Eclipse XDB-C18, the mobile phase was methanol-water (62: 38) V / V / V, the flow rate was 1.0 mL / min, the detection wavelength was 307 nm, the column temperature was 30 鈩，

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