颊针疗法对类风湿性关节炎兔的镇痛时效及其作用机制

发布时间：2018-03-21 04:03

本文选题：针刺疗法　切入点：类风湿性关节炎　出处：《中南大学学报(医学版)》2017年05期 　论文类型：期刊论文

【摘要】：目的:探究针刺对患有类风湿性关节炎大耳白兔的镇痛时效及其中枢作用机制。方法:将60只大耳白兔随机分为正常组(n=6)、模型组(n=6)、体针组(n=24)和颊针组(n=24),再将后两组分为针后即时(0 h)、针后0.5,1,2 h 4个亚组,每组各6只。白兔类风湿性关节炎模型采用卵蛋白诱导建立。体针组针刺双侧"膝眼"和"足三里"1次,颊针组双侧颊针针刺"膝"1次,行针15 s,留针30 min。采用PL-200热刺痛仪检测痛阈,以兔出现缩耳挣扎动作为疼痛反应指标,自照射开始至兔出现反应的潜伏期时间作为该兔的痛阈值;使用放射免疫法检测兔脑脊液中β-内啡肽(β-endorplhin,β-EP)及八肽胆囊收缩素(cholecystokinin-8,CCK-8)含量。结果:与正常组相比,模型组痛阈和CCK-8含量显著降低(P0.01),β-EP含量显著升高(P0.05);与模型组相比,体针组和颊针组的痛阈在0和1 h(P0.05或P0.01)显著升高;体针组和颊针组兔脑脊液中CCK-8和β-EP含量均显著高于模型组(P0.01或P0.05);颊针组的即时(0 h)CCK-8和β-EP含量显著高于体针组(P0.05),其他时间无明显变化。结论:针刺治疗可以有效缓解类风湿性关节炎的疼痛,颊针疗法的即时镇痛效应优于体针治疗,且这种即时镇痛效应与脑脊液中β-EP和CCK-8含量变化相关。
[Abstract]:Objective: to explore the analgesic effect and central mechanism of acupuncture on rabbits with rheumatoid arthritis. Methods: 60 rabbits were randomly divided into normal group, model group and buccal acupuncture group. The group was divided into 4 subgroups, one hour after acupuncture and one hour after acupuncture, and four subgroups were divided into 4 groups. There were 6 rabbits in each group. The rabbit model of rheumatoid arthritis was induced by ovalbumin. The body acupuncture group needled the bilateral "knee eyes" and "Zusanli" once, and the buccal acupuncture group the bilateral buccal acupuncture "knee" once. The pain threshold was measured by PL-200 thermal tingling instrument. The pain threshold was determined by the pain response index of the rabbit's ear contraction and struggling, and the latency period from the beginning of irradiation to the rabbit's reaction time was used as the pain threshold. The contents of 尾 -endorphin (尾 -EP) and cholecystokinin-8 (CCK-8) in cerebrospinal fluid of rabbits were measured by radioimmunoassay. Results: compared with the normal group, the pain threshold and CCK-8 content in the model group were significantly lower than those in the normal group, and the content of 尾 -EP was significantly higher than that in the model group. The pain threshold of body acupuncture group and buccal acupuncture group increased significantly at 0 and 1 h (P0.05 or P0.01). The contents of CCK-8 and 尾 -EP in cerebrospinal fluid of rabbits in body acupuncture group and buccal acupuncture group were significantly higher than those in model group (P0.01 or P0.05), and the contents of CCK-8 and 尾 -EP in buccal acupuncture group were significantly higher than those in body acupuncture group at 0 h. The pain of rheumatoid arthritis, The immediate analgesic effect of buccal acupuncture was better than that of body acupuncture, and the immediate analgesic effect was related to the changes of 尾 -EP and CCK-8 in cerebrospinal fluid.
【作者单位】：甘肃中医药大学针灸推拿学院;天水市中医医院针灸科;
【基金】：国家自然科学基金(81260559)~~
【分类号】：R245

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