基于悬液微珠抗体芯片技术的肝癌和大肠癌术后“同病异证”“异病同证”分析

发布时间：2018-03-21 04:20

本文选题：肝癌　切入点：大肠癌　出处：《中华中医药杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的:揭示肝癌和大肠癌术后"同病异证""异病同证"的特征性差异表达的细胞因子。方法:运用悬液微珠抗体芯片技术,定量检测分析证候间45种细胞因子的差异表达谱;运用ELISA对TGF-β1进行定量检测,并对显著差异因子进行验证。结果:通过统计分析发现,IP-10、RANTES、MIP-1β、IL-18、IL-1RA以及IFN-γ在肝癌术后,RANTES、BDNF、PDGF-BB、IL-9以及FGF-2在肠癌术后的肝肾阴虚证、脾虚证、湿热证和隐证中表达差异有统计学意义(P0.05)。与隐证相比,肝癌和大肠癌术后肝肾阴虚证、脾虚证和湿热证各具有不同的差异表达细胞因子谱。肝癌和大肠癌术后肝肾阴虚证共同的特异性细胞因子为RANTES,脾虚证共同的特异性细胞因子为TGF-β1,但湿热证中未发现共有的异病同证因子。对肝癌和大肠癌术后的共有显著差异细胞因子进行拟合和ROC分析,发现肝肾阴虚证的AUC值为0.837,脾虚证的AUC值为0.871,湿热证的AUC值为0.833,隐证的AUC值为0.936。生物学通路分析发现,Cytokine-cytokine receptor interaction、Cytosolic DNA-sensing pathway、Influenza A和Toll-like receptor signaling pathway等信号通路与肝癌术后同病异证相关;MAPK signaling pathway、Cytokine-cytokine receptor interaction以及Melanoma信号通路与肠癌术后同病异证相关。结论:肝癌和大肠癌术后肝肾阴虚证、脾虚证和湿热证有特异性细胞因子,这些细胞因子的变化可能与其证候的形成有关。
[Abstract]:Objective: to reveal the characteristic differential expression of cytokines in patients with liver cancer and large intestine cancer after operation. Methods: the differential expression profiles of 45 cytokines in different syndromes were quantitatively detected and analyzed by suspension microsphere antibody chip technique. TGF- 尾 1 was quantitatively detected by ELISA, and the significant difference factors were verified. Results: it was found that IP-10 RANTESU MIP-1 尾 IL-18 IL-1RA and IFN- 纬 were used to detect IL-9 of PDGF-BBGF-9 and FGF-2 in liver and kidney yin deficiency syndrome, spleen deficiency syndrome after liver cancer operation. The difference of expression between dampness and heat syndrome and hidden syndrome was statistically significant (P 0.05). Compared with hidden syndrome, liver and kidney yin deficiency syndrome after hepatocarcinoma and colorectal cancer operation, The specific cytokines of liver and kidney yin deficiency syndrome were RANTESand the specific cytokine of spleen deficiency syndrome was TGF- 尾 1, but there was no occurrence in damp-heat syndrome. There are common syndromes of different diseases. There are significant differences in cytokines between liver cancer and large intestine cancer after operation. The results of fitting and ROC analysis are as follows. It was found that the AUC value of liver and kidney yin deficiency syndrome was 0.837, the AUC value of spleen deficiency syndrome was 0.881, the AUC value of damp-heat syndrome was 0.833, and the AUC value of hidden syndrome was 0.936. The signal pathways such as Cytokine-cytokine-cytokine receptor intervention cytosolic DNA-sensing pathwayInfluenza A and Toll-like receptor signaling pathway were found to be different from those of liver cancer after operation. MAPK signaling pathway-Cytokine-cytokine receptor interaction and Melanoma signal pathway are related to the syndromes of the same disease after operation of colorectal cancer. Conclusion: liver and kidney yin deficiency syndrome after operation of liver cancer and large intestine carcinoma. Spleen deficiency syndrome and damp-heat syndrome have specific cytokines, and the changes of these cytokines may be related to the formation of their syndromes.
【作者单位】：上海中医药大学中医复杂系统研究中心;
【基金】：国家自然科学基金重点项目(No.81330084) 上海市教委E-研究院中医内科建设计划项目(No.E03008)~~
【分类号】：R273

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