平胃散治疗湿困脾胃证的化学物质基础研究

发布时间：2018-03-21 21:45

本文选题：平胃散　切入点：湿困脾胃　出处：《成都中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：本文由五部分组成,第一部分平胃散治疗湿困脾胃证的有效部位筛选；第二部分平胃散主要有效部位化学成分研究；第三部分单体化合物来源分析；第四部分以臣药厚朴为主的复方配伍规律研究；第五部分平胃散主要化学成分的含量测定。第一部分采用D~(-1)01大孔树脂对平胃散水煎液进行分段,分为水部位、20%乙醇部位、50%乙醇部位、75%乙醇部位、95%7醇部位。用湿阻中焦大鼠模型筛选有效部位。实验结果表明,平胃散水煎液的不同部位对于湿阻中焦模型大鼠的胃肠功能均有一定的调节作用,综合肠推进率、胃残留率以及D-木糖的吸收,将50%乙醇部位推断为平胃散治疗湿困脾胃证的主要有效部位。第二部分采用传统的柱色谱(硅胶、葡聚糖凝胶、反相Cls等)结合制备液相等对50%7醇部位进行化合物的分离纯化,从50%乙醇部位分离、纯化、鉴定了8个化合物,分别是：化合物1(甘草苷)、化合物2(厚朴酚)、化合物3(异甘草素)、化合物4(甘草素)、化合物5(异甘草苷)、化合物6(和厚朴酚)、化合物7(甘草酸)、化合物8(橙皮苷)。以上化合物均首次从平胃散水煎液中分离得到。第三部分对分离纯化得到的化合物进行来源归属。利用HPLC法对平胃散及已分离纯化的化合物进行分析,结果平胃散中5个特征色谱峰代表的成分分别是：甘草苷、橙皮苷、甘草酸、和厚朴酚、厚朴酚。通过与单味药对比归属了其来源分别是：和厚朴酚、厚朴酚来自臣药厚朴；橙皮苷来自佐药陈皮;甘草苷、甘草酸来自使药甘草。第四部分利用HPLC法以臣药厚朴为主研究复方的配伍规律。实验结果表明,“君药苍术”及生姜、大枣可以使平胃散中厚朴酚与和厚朴酚的含量降低。“佐药陈皮”。及“使药甘草”可以使平胃散中厚朴酚与和厚朴酚的含量升高；君药苍术及生姜、大枣使厚朴酚与和厚朴酚在平胃散中降低的趋势大于佐药陈皮及使药甘草使其升高的趋势。第五部分对平胃散中主要成分：橙皮苷、甘草酸、厚朴酚、和厚朴酚进行了含量测定。各成分在复方中的含量依次是：橙皮苷1.248mg-g~(-1)、甘草酸0.869mg.g~(-1)、和厚朴酚1.500mg.g~(-1)、厚朴酚0.104mg.g~(-1)。
[Abstract]:This article consists of five parts: the first part is the screening of the effective parts of Pingwei Powder in treating dampness of spleen and stomach syndrome, the second part is the study of the chemical constituents of the main effective parts of Pingwei Powder, the third part is the analysis of the source of the monomer compounds. The fourth part is about the law of compound compatibility of Magnolia officinalis, the fifth part is the determination of the main chemical components of Pingwei Powder. It can be divided into two parts: water, 20% ethanol, 50% ethanol, 75% ethanol, 95 7 alcohol. The effective parts were screened by the model of wet-blocking medium scorch rats. The experimental results showed that, The different parts of Pingwei Powder decoction could regulate the gastrointestinal function of rats with dampness obstruction and middle Jiao model, such as the comprehensive intestinal propulsion rate, gastric residual rate and absorption of D-xylose. In the second part, the traditional column chromatography (silica gel, dextran gel, reversed phase Cls, etc.) was used to prepare liquid phase for the separation and purification of 507 alcohols. Eight compounds were isolated, purified and identified from the ethanol fraction of 50%. Compounds 1 (glycyrrhizin), 2 (honokiol), 3 (isoglycyrrhizin), 4 (glycyrrhizin), 5 (isoglycyrrhizin), 6 (and honokiol), 7 (glycyrrhizinate, 8 (hesperidin)). The above compounds were isolated from the decoction of Pingwei Powder for the first time. In the third part, the source of the purified compounds was assigned. The HPLC method was used to analyze Pingwei Powder and the compounds that had been separated and purified. Results the representative components of five characteristic chromatographic peaks in Pingwei Powder were: glycyrrhizin, hesperidin, glycyrrhizic acid, honokiol and honokiol. Hesperidin comes from the adjunct peel; glycyrrhizin, glycyrrhizic acid from licorice. The fourth part uses HPLC method to study the compatibility rule of compound compound with Magnolia officinalis. Jujube can reduce the contents of honokiol and honokiol in Pingwei Powder. The contents of honokiol and honokiol in Pingwei Powder can be increased by "adjuvant drug Chen Pi" and "liquorice medicine", and the content of magnolol and honokiol in Pingwei powder can be increased. The decreasing trend of honokiol and honokiol in Pingwei Powder was greater than that of adjunct and liquorice. Part 5: hesperidin, glycyrrhizic acid, honokiol, and magnolol in Pingwei Powder, the main components in Pingwei Powder were: hesperidin, glycyrrhizic acid and honokiol. The contents of honokiol and honokiol were determined in order of hesperidin 1.248 mg 路g ~ (-1), glycyrrhizic acid 0.869 mg 路g 路g ~ (-1), honokiol 1.500 mg 路g ~ (-1) and honokiol 0.104 mg 路g ~ (-1).
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

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