针刺对自发性高血压大鼠的降压效应及其中枢Asp、Glu调节机制

发布时间：2018-03-28 18:49

本文选题：针刺　切入点：自发性高血压大鼠　出处：《浙江中医药大学》2017年硕士论文

【摘要】：目的以自发性高血压大鼠(SHR)为研究载体,观察以"太冲"为主穴的不同经穴配伍(原络配穴、同名经配穴、辨证配穴)对SHR血压的影响,确定降压效应最佳配穴组方;观察针刺对SHR延髓头端腹外侧区(RVLM)天冬氨酸(AsP)、谷氨酸(Glu)的影响,初步探讨针刺降压的部分中枢作用机制。方法SHR 72只,随机分为6组:模型组、太冲配内关组、太冲配蠡沟组、太冲配光明组、太冲配太溪组、太冲配非穴组,每组12只,另设同周龄WKY大鼠12只作为正常对照组。各治疗组针刺相应双侧穴位,每次30min,每天1次,共15次;模型组及WKY组不予针刺。分别于治疗第1、3、7、15天针刺结束后测量各组大鼠尾动脉血压。模型组、太冲配内关组、太冲配非穴组于第15天测完血压后立即处死,正常对照组大鼠同一天处死,用高效液相荧光法(HPLD-FLD)测定各组大鼠RVLM中Asp、Glu含量。结果一、大鼠血压变化:1.收缩压(SBP):针刺7天、15天后,与模型组比较,太冲配内关组、太冲配光明组、太冲配太溪组、太冲配非穴组SBP均明显下降,差异有统计学意义(P0.05);针刺15天后,太冲配蠡沟组SBP降压百分比大于模型组(P0.05),太冲配内关组SBP降压百分比大于太冲配蠡沟组、太冲配光明组、太冲配太溪组、太冲配非穴组(P0.05);与针刺前比较,太冲配内关组在针刺15天后SBP、舒张压(DBP)、平均动脉压(MBP)均明显下降,差异有统计学意义(P0.05);2.DBP:针刺7天、15天后,太冲配内关组DBP降压百分比大于模型组,差异有统计学意义(P0.05);3.MBP:针刺7天、15天后,太冲配内关组MBP降压百分比大于模型组,差异有统计学意义(P0.05);二、大鼠RVLM中Asp、Glu含量差异:与正常对照组比较,模型组Asp、Glu含量明显升高,差异有统计学意义(P0.05);太冲配内关组Asp、Glu含量明显低于模型组,差异有统计学意义(P0.05);太冲配内关组Asp含量明显低于太冲配非穴组,差异有统计学意义(P0.05),太冲配内关组Glu含量较太冲配非穴组低,但差异无统计学意义(P0.05)。结论针刺具有降压效应,以"太冲"为主穴的原络配穴、同名经配穴、辨证配穴,对SHR的血压均有调控作用,其中"太冲"配"内关"穴(同名经配穴)针刺降压效应最佳;针刺降压的中枢机制可能是通过影响大鼠RVLM中Asp、Glu实现的。
[Abstract]:Objective to observe the effect of different meridian combinations (original collaterals, syndromes, syndrome differentiation) on the blood pressure of SHR with "Taicong" as the main point. To observe the effect of acupuncture on aspartic acid aspartic acid (ASP) and glutamic acid (Glul) in the ventrolateral ventrolateral medulla of SHR, and to explore the mechanism of partial central effect of acupuncture on reducing blood pressure. Methods 72 rats of SHR were randomly divided into 6 groups: model group, Taichong plus Neiguan group, Taichong Baiguo group, Taichong Baiguo group. There were 12 rats in each group in Taichong combined with Taixi group, Taichong plus non-point group and 12 WKY rats of the same age as normal control group. Each treatment group was treated with acupuncture at corresponding bilateral acupoints for 30 minutes, once a day for 15 times. The blood pressure of caudal artery in each group was measured at the end of acupuncture at the end of 715 days after treatment. The model group, Taichong plus Neiguan group and Taichong plus non-point group were killed immediately after the blood pressure was measured on the 15th day. The rats in the control group were killed on the same day, and the content of Asp1 Glu in RVLM was determined by high performance liquid phase fluorescence method. Results 1. The blood pressure of rats changed to 1. Systolic blood pressure (SBP): after 7 days and 15 days acupuncture, compared with the model group, the rats were compared with Neiguan group. The SBP of Taichong group, Taichong and Taixi group, Taichong group and non-point group were significantly decreased (P 0.05), and the difference was significant after 15 days of acupuncture. The percentage of SBP hypotension in Taichong and Ligou group was higher than that in model group, and the percentage of SBP in Taichong and Neiguan group was higher than that in Taichong and Baiguang group, Taichong and Taixi group, Taichong and non-acupoint group, compared with that before acupuncture, the percentage of SBP in Taichong and Neiguan group was higher than that in Taichong Peiguang group, Taichong and Taixi group. After 15 days of acupuncture, SBP, DBP, and mean arterial pressure in Taichong and Neiguan group were significantly decreased, the difference was statistically significant (P 0.05) and 2.DBP: after 7 days and 15 days of acupuncture, the percentage of DBP in Taichong and Neiguan group was higher than that in model group. After 7 days and 15 days of acupuncture, the percentage of MBP in Taichong and Neiguan group was higher than that in model group, and the difference was statistically significant (P 0.05). Second, the content of AspP in RVLM in rats was significantly higher than that in normal control group. The content of Asp in Taichong Neiguan group was significantly lower than that in model group (P 0.05), and the content of Asp in Taichong injection Neiguan group was significantly lower than that in Taichong Neiguan group. The content of Glu in Taichong and Neiguan group was lower than that in Taichong and non-point group, but the difference was not statistically significant. Conclusion Acupuncture has the effect of lowering blood pressure. The blood pressure of SHR was regulated, among which "Taicong" combined with "Neiguan" point had the best effect of lowering blood pressure, and the central mechanism of acupuncture on blood pressure might be realized by affecting AspGlu-Glu in rat RVLM.
【学位授予单位】：浙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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