早、中期慢性肾衰竭患者经肠道治疗后肠道菌群及中医证候积分的变化

发布时间：2018-03-31 23:22

本文选题：慢性肾衰竭　切入点：中药灌肠　出处：《新疆医科大学》2017年硕士论文

【摘要】：目的:本研究通过罗氏454高通量测序法,观察经肠道治疗后患者肠道菌群及中医证候的变化,进一步发现经肠道治疗CRF的作用靶点和作用机制。方法:1)将来自符合纳入标准新疆自治区中医院肾病科住院的60例患者,按照患者意愿分为治疗组31例、对照组29例,平衡患者一般资料后进行统计学分析。两组均给予西医常规治疗,治疗组在西医常规基础上联合中药保留灌肠治疗,2周为一个疗程,4周后比较2组患者中医证候的变化;2)每组各抽取10例患者,留取治疗前、治疗2周后、治疗4周后的3个时期的粪便,对粪便标本进行高通量测序检测,观察两组患者的肠道菌群的变化。结果:治疗4周后,两组患者的中医证候均有所改善,治疗组在证候疗效、证候积分上明显优于对照组;具有统计学意义(P0.05)。两组患者治疗前后肠道菌群测序的数据经过统计学分析在门的水平上未见有意义的变化,在属的水平上的双歧杆菌、大肠杆菌、乳酸菌发生了有意义的变化。结论:经肠道治疗可对慢性肾衰竭患者的肠道菌群产生影响,其与肠道菌群密切相关。
[Abstract]:Objective: to observe the changes of intestinal flora and TCM syndromes in patients with CRF after intestinal therapy by means of high throughput sequencing of Roche 454, and to find out the action target and mechanism of enteral treatment of CRF.Methods 60 patients from renal department of Xinjiang Autonomous region Chinese Medicine Hospital were divided into treatment group (31 cases) and control group (29 cases) according to the wishes of the patients.Both groups were given routine western medicine treatment. The treatment group was treated with traditional Chinese medicine retention enema on the basis of western medicine routine. After 2 weeks of treatment and 4 weeks of treatment, the changes of TCM syndromes in both groups were compared.After 2 weeks and 4 weeks of treatment, the stool samples were detected by high throughput sequencing, and the changes of intestinal flora of the two groups were observed.Results: after 4 weeks of treatment, the TCM syndromes of the two groups were improved, and the treatment group was superior to the control group in syndromes and syndromes integral, which had statistical significance (P 0.05).Two groups of patients before and after treatment of intestinal flora sequencing data after statistical analysis at the gate level no significant changes in the generic level of Bifidobacterium, Escherichia coli, lactic acid bacteria have a significant change.Conclusion: intestinal microflora in patients with chronic renal failure can be affected by intestinal therapy, which is closely related to intestinal flora.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R277.5

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