脾虚泄泻患者肠道念珠菌群的生态变化研究

发布时间：2018-04-04 13:21

本文选题：脾虚　切入点：泄泻　出处：《辽宁中医杂志》2017年08期

【摘要】：目的:探讨脾虚泄泻患者肠道念珠菌群的生态变化,为脾虚泄泻证的防治提供理论依据。方法:受试者分为正常对照组和脾虚泄泻组,共两组,每组40例。无菌称取受试者新鲜粪便标本,稀释至所需浓度后,分别取0.05 m L、0.1m L接种于科玛嘉念珠菌显色培养基和沙保氏培养基表面,培养后计数种类及菌落数。结果:脾虚泄泻组粪便中的念珠菌检出率显著高于正常对照组,其中白色念珠菌检出率高达82.5%,男性患者粪便中的光滑念珠菌和克柔念珠菌检出率明显高于女性;沙保氏培养基表面生长的粪便活菌数明显高于正常对照组(P0.01)。结论:脾虚泄泻患者的肠道群菌失调,粪便中念珠菌种类检出率增高,活菌数明显多于正常健康者。
[Abstract]:Objective: to study the ecological changes of intestinal Candida flora in patients with spleen deficiency diarrhea and to provide theoretical basis for the prevention and treatment of spleen deficiency diarrhea syndrome.Methods: the subjects were divided into normal control group and spleen asthenia diarrhea group with 40 cases in each group.Fresh fecal specimens were collected and diluted to the required concentration. 0.05 mL / L 0.1m L was inoculated on the surface of Color-developing medium and Saab's medium respectively. The species and colony number were counted after culture.Results: the positive rate of Candida albicans in feces of spleen deficiency diarrhea group was significantly higher than that of normal control group, and the positive rate of Candida albicans in male patients was 82.5. The positive rate of Candida smooth and Candida krolimus in feces of male patients was significantly higher than that of female patients.The number of fecal living bacteria on the surface of Sabao's medium was significantly higher than that of the normal control group (P 0.01).Conclusion: in patients with diarrhea due to spleen deficiency, the intestinal flora was disordered, the positive rate of Candida species in feces was increased, and the number of living bacteria was significantly higher than that of normal controls.
【作者单位】：辽宁中医药大学;
【基金】：国家自然科学基金项目(81173145)
【分类号】：R256.34

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