针刺联合美多芭对帕金森病小鼠脑多巴胺神经元及蛋白激酶B表达的影响

发布时间：2018-04-11 21:45

本文选题：帕金森病 + -甲基--苯基-　；参考：《北京中医药大学学报》2017年03期

【摘要】：目的通过观察针刺舞蹈震颤控制区联合美多芭对帕金森病(PD)小鼠脑内蛋白激酶B(Akt)表达的影响来探讨针刺联合美多芭对多巴胺(DA)能神经元的保护作用及可能机制。方法将C57BL/6雄性小鼠随机分为正常组、模型组、美多芭组、针刺组和针刺联合美多芭(简称针美)组。应用腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)的方法建立PD小鼠模型,后3组在造模后采取不同治疗措施,造模后小鼠存活28 d。采用爬杆法观察小鼠行为学;免疫组织化学技术检测小鼠中脑黑质致密部多巴胺神经元的丢失程度及脑Akt的表达。结果 3个治疗组小鼠行为学爬杆时间均较模型组有明显缩短(P0.05)。造模后,小鼠黑质致密部多巴胺神经元表达较正常组减少(P0.05),其中针美组多巴胺神经元表达较模型组及美多芭组增多(P0.05)。在黑质致密部,针美组Akt与其余各组相比,表达明显升高(P0.05);在海马部位,针美组在CA1、CA3和CA4区的表达比模型组和美多芭组明显升高(P0.05),在CA1和CA4的表达比针刺组有明显上升(P0.05)。结论针刺舞蹈震颤控制区特别是再给予美多芭复合治疗可能通过调节脑磷酯酰肌醇-3-羟激酶(PI3K)/Akt通路,从而达到治疗PD的目的。
[Abstract]:Objective to investigate the protective effect and possible mechanism of acupuncture combined with MDB on dopaminergic neurons in the brain of Parkinson's disease (PD) mice.Methods C57BL/6 male mice were randomly divided into normal group, model group, medoba group, acupuncture group and acupuncture combined with medoba group.The PD mice model was established by intraperitoneal injection of 1-methyl-4-phenyl-1-trihydropyridine (MPTP). The last three groups were treated with different treatment measures and survived for 28 days.The loss of dopamine neurons in the substantia nigra compact region and the expression of Akt in the brain were detected by the method of climbing rod and immunohistochemistry.Results compared with the model group, the behavioral climbing time of the three treatment groups was significantly shorter than that of the model group.After modeling, the expression of dopamine neurons in the substantia nigra compact region was lower than that in the normal control group, and the expression of dopamine neurons in the acupuncture group was higher than that in the model group and medoba group.Conclusion Acupuncture in the dance tremor control area, especially in the treatment of medoba, may be achieved by regulating the PI3K / Akt pathway of phosphoinositide -3-hydroxyl kinase in the brain, so as to achieve the purpose of the treatment of PD.
【作者单位】：北京中医药大学基础医学院;
【基金】：国家自然科学基金资助项目(No.81573773) 北京中医药大学校级自主课题项目(No.2011-JYB22-JS063)
【分类号】：R245

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