电针对去卵巢大鼠骨形成及骨吸收活性的影响

发布时间：2018-04-12 10:23

  本文选题：电针 + 骨质疏松症　； 参考：《中国骨质疏松杂志》2017年05期

【摘要】：目的研究电针刺对去卵巢骨质疏松模型大鼠的全身骨密度(BMD)、骨矿物含量(BMC)及其血清对体外培养成骨细胞的骨形成因子骨钙素(OPG)和骨吸收因子(RANKL)蛋白表达的影响。方法选取SPF级2月龄SD雌性大鼠40只,随机分为空白组、电针组、药物组和模型组,每组10只。其中空白组不做任何处理,后3组采用手术方法切除双侧卵巢。饲养3个月后,骨密度检测结果显示造模成功。随后药物组大鼠灌胃服用戊酸雌二醇3个疗程,电针组大鼠采用针刺后通电治疗3个疗程并服用药物组相同体积的蒸馏水,模型组服用相应体积的蒸馏水,空白组常规饲养。各组大鼠于SPF级实验室处理3个月后,双能X射线骨密度仪检测全身骨密度和骨矿含量。随后,水合氯醛麻醉各组大鼠,心脏采血,各组血液分别经滤网过滤、56℃水浴处理后加入体外培养的大鼠成骨细胞培养液中,处理3d后多聚甲醛固定成骨细胞,采用免疫细胞化学染色法检测成骨细胞内OPG、RANKL蛋白的表达量。结果与空白组相比,模型组大鼠BMD,BMC显著降低(P0.05),服用药物或针刺处理后,BMD和BMC显著增高(P0.05),与模型组相比差异具有统计学意义。各组大鼠血清处理成骨细胞后,模型组骨形成指标OPG蛋白表达量较低,经药物或针刺后,OPG蛋白表达量显著升高(P0.01)。此外,模型组骨吸收指标RANKL的表达量较高,经药物和针刺处理后,RANKL蛋白表达量显著下降(P0.01)。结论针刺能有效升高大鼠BMD和BMC,同时针刺处理后的大鼠血清具有很好的促进骨形成和抑制骨吸收的能力。
[Abstract]:Objective to study the effects of electroacupuncture (EA) on the expression of bone morphogenetic factor osteocalcin (OPG) and bone resorption factor (RANKL) in osteoblasts of ovariectomized rats with bone mineral content (BMC) and bone mineral content (BMC).Methods Forty SPF 2-month-old SD female rats were randomly divided into blank group, electroacupuncture group, drug group and model group with 10 rats in each group.The blank group did not do any treatment, and the latter 3 groups underwent bilateral ovary resection.After feeding for 3 months, bone mineral density test showed that the model was successful.Then the rats in the drug group were treated with estradiol valerate for 3 courses, the rats in the electroacupuncture group were treated with electricity after acupuncture for 3 courses and the same volume of distilled water was taken in the drug group, the model group was given the corresponding volume of distilled water, and the blank group was fed routinely.The bone mineral density and bone mineral content were measured by dual energy X ray absorptiometry after 3 months of SPF laboratory treatment.Then, the rats were anesthetized by chloral hydrate, and the blood was collected from the heart. The blood of each group was treated with filter filter at 56 鈩，

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