膝痹膏摩法对兔膝骨性关节炎滑膜MMP-1、MMP-3、MMP-13影响的研究

发布时间：2018-04-12 11:05

本文选题：膝骨性关节炎 + 膝痹膏摩法　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:通过观察膝痹膏摩法对兔膝骨性关节炎滑膜中MMP-1、MMP-3、MMP-13水平的影响,对探究膝痹膏摩法治疗膝骨性关节炎的作用机制提供实验依据。方法:新西兰大白兔43只,随机分出10只作为正常组,将剩余33只利用石膏铁丝固定法建立兔膝骨性关节炎模型,造模8周,随机处死3只兔子查看造模情况,造模成功,将30只兔子随机分成模型组、手法组、膝痹膏摩组,每组10只,正常组和模型组正常饲养,不做干预;手法组给予推拿手法治疗每天一次,治疗8周;膝痹膏摩组给予膝痹膏配合推拿手法治疗每天一次,治疗8周。治疗结束后取材,通过大体观察关节面和关节软骨行HE病理染色观察软骨表面组织学形态变化情况;滑膜采用双抗体夹心酶联免疫吸附法(ELISA)检测各组滑膜组织中MMP-1、MMP-3、MMP-13的表达情况。结果:大体观察及光镜下观察软骨表面:与模型组相比,手法组和膝痹膏摩组软骨损伤程度较轻,其中膝痹膏摩组损伤程度最轻,大体评分膝痹膏摩组和模型组比较有统计学意义(p0.05)。膝关节软骨Mankin评分:模型组膝关节软骨Mankin评分显著高于其他各组(p0.01);而手法组及膝痹膏摩组与模型组比较膝关节软骨Mankin评分显著降低(p0.01),其中膝痹膏摩组膝关节软骨Mankin评分较手法组更低(p0.05)。结果显示模型组中MMP-1、MMP-3、MMP-13含量均高于其余各组(P0.01),正常组中MMP-1、MMP-3、MMP-13含量最低(P0.01)。各组间比较:膝痹膏摩组MMP-13的含量明显低于手法组(P0.01);膝痹膏摩组MMP-1、MMP-3的含量和手法组比较有统计学意义(P0.05)。结论:膝痹膏摩法治疗膝骨性关节炎可能是通过抑制MMP-1、MMP-3、MMP-13酶的活性而延缓对软骨及滑膜的破坏,从而减慢对关节组织的损害程度,发挥治疗膝骨性关节炎的作用。
[Abstract]:Objective: to observe the effect of knee arthralgia on the level of MMP-1, MMP-3 and MMP-13 in the synovium of rabbit knee osteoarthritis, and to provide experimental basis for exploring the mechanism of knee arthritic arthritis treated by knee arthralgia.Methods: 43 New Zealand white rabbits were randomly divided into 10 normal groups. The remaining 33 rabbits were used to establish the rabbit knee osteoarthritis model by the method of plaster wire fixation.30 rabbits were randomly divided into model group, manipulation group, knee arthralgia group with 10 rabbits in each group, normal feeding group and model group without intervention, manipulation group was treated with massage manipulation once a day for 8 weeks.The treatment group was treated with knee arthralgia ointment and massage manipulation once a day for 8 weeks.After treatment, histopathological changes of articular surface and articular cartilage were observed by HE pathological staining, and the expression of MMP-1, MMP-3 and MMP-13 in synovial tissue were detected by double antibody sandwich enzyme linked immunosorbent assay (Elisa).Results: compared with the model group, the degree of cartilage injury was lighter in manipulation group and knee arthralgia group, especially in knee arthralgia group.The gross score of the two groups was significantly higher than that of the model group (P 0.05).The Mankin score was lower than that in the manipulation group (P 0.05).The results showed that the content of MMP-1, MMP-3, MMP-13 in the model group was higher than that in the other groups, and the content of MMP-1, MMP-3, MMP-13 in the normal group was the lowest (P 0.01).Comparison among groups: the content of MMP-13 was significantly lower in group A than that in group B (P 0.01), and the content of MMP-1 + MMP-3 in group B was significantly lower than that in group B (P 0.05).Conclusion: the treatment of knee osteoarthritis with knee arthralgia may delay the destruction of cartilage and synovium by inhibiting the activity of MMP-1, MMP-3 and MMP-13, so as to slow down the damage to joint tissue and play a role in the treatment of knee osteoarthritis.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R244.1

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