中医调护PTSD大鼠“胎损”子代行为及相关神经递质研究

发布时间：2018-04-20 20:50

本文选题：创伤后应激障碍 + 胎损　；参考：《成都中医药大学》2016年硕士论文

【摘要】：[目的]1.通过观测创伤后应激心理障碍孕鼠“胎损”子代出生情况、生长发育、行为表现、血清神经递质,研究“胎损”对SD孕鼠子代的行为遗传及神经生物学损害；2.正确评估补肾中药金匮肾气丸联合优裕环境情志调护措施对创伤后应激心理障碍孕鼠“胎损”致子代生长发育迟缓、行为表现异常及血清神经递质紊乱的干预效果,科学阐释“母病及子”、“恐伤肾”、“肾主生殖”、“肾主骨生髓”等经典中医理论内涵。[方法]将初次受孕的SD孕鼠(36只)采用随机数字表分为三组：对照组、PTSD组以及情志+中药组(以下简称情中组),每组12只。PTSD组大鼠和情中组大鼠于孕中期(第7-13d)采用单程长时应激法进行创伤应激造模。妊娠14天至分娩,PTSD组和对照组孕鼠正常实验条件下普通饲养笼一般饲料常规喂养,情中组孕鼠给予补肾中药金匮肾气丸及优裕环境情志调护措施。记录各组孕鼠妊娠并观察有无流产及早产状况；重复测量各组子鼠体重、身长、尾长(出生第0、5、15、25d)、张耳(出生第5d)等生长发育指标；在不同时间段对各组子鼠进行行为能力测试,包括翻身测试(出生60s内)、亮盒排便测试(子鼠出生第20d、30d)、糖水消耗试验(子鼠出生第26d)、高架十字迷宫实验(子鼠出生第30d)。股动脉采血(子鼠出生第30d),采用酶联免疫吸附法对子代鼠的皮质酮、5-羟色胺、多巴胺等血清神经递质水平进行检测。[结果]1.生长发育指标各组孕鼠都正常分娩,均无早产及流产情况。PTSD组出生体重、身长、尾长均低于对照组和情中组,差异有统计学意义(P0.05)；情中组和对照组出生体重比较差异有统计学意义(P0.05),身长、尾长比较差异无统计学意义(P0.05)对子鼠第5、15、25天体重、身长、尾长动态连续测量,各组之间体重、身长、尾长差异均有统计学意义(P组间0.05)。体重、身长、尾长受时间因素影响,不同时间点上体重、身长、尾长比较,差异有统计学意义(P时间0.05)；时间因素和分组因素的交互作用具有统计学意义(P交互0.05),即体重、身长、尾长随时间变化的趋势因分组不同而不同。通过各时间点各个指标比较得出：PTSD组生长发育最慢,而情中组生长发育较快且优于对照组。PTSD组和对照组张耳情况比较,差异无统计学意义(P0.05)；情中组张耳发育优于对照组和PTSD组,差异有统计学意义(P0.05)2.行为能力测试翻身测试中,PTSD组翻身能力低于对照组和情中组,差异有统计学意义(P0.01)；情中组和对照组翻身能力比较,差异无统计学意义(P0.05)。亮盒排便测试中,PTSD组大小便粒／次均高于其余两组,差异有统计学意义(P0.05)；情中组与对照组比较,大便粒数差异有统计学意义(P0.05),小便次数差异无统计学意义(P0.05)。重复测量时间比较,时间差异有统计学意义(P0.05)。糖水消耗试验中,PTSD组糖水偏嗜度低于情中组,差异有统计学意义(P0.01)；情中组和对照组糖水偏嗜度比较,差异无统计学意义(P0.05)高架十字迷宫实验中,PTSD组开臂进入次数百分比和开臂停留时间百分比低于情中组和对照组,差异有统计学意义(P0.05)；情中组开臂进入次数百分比和开臂停留时间百分比均高于对照组,差异有统计学意义(P0.05)。3.血清神经递质PTSD组血清皮质酮水平高于情中组和对照组,差异有统计学意义(P0.05)；情中组和对照组皮质酮比较,差异无统计学意义(P0.05)。PTSD组血清5-羟色胺水平低于情中组和对照组,差异有统计学意义(P0.05)；情中组5-羟色胺水平高于对照组,差异有统计学意义(P0.05)。PTSD组血清多巴胺水平低于对照组,差异有统计学意义(P0.05)；情中组和对照组多巴胺水平比较,差异无统计学意义(P0.05)。[结论]1.母鼠孕期遭受创伤恐惧应激的危害会遗传到子代,导致子代低出生体重(身长尾长)、生长发育迟缓、行为表现及血清神经递质异常等。2.中医调护措施可调节并纠正PTSD'‘胎损”致子代生长发育迟缓、行为能力下降及神经内分泌紊乱等。3.“恐伤肾”是PTSD“胎损”的病机之一。对“胎损”子代神经生物学调节可通过先天补肾精、温肾阳及后天疏肝理气,达到气血生化充足实现。
[Abstract]:Objective : To study the genetic and neurobiological damage of the offspring of SD pregnant rats by observing the birth , development , behavior and neurotransmitters in the offspring of pregnant rats with stress after trauma .
2 . To evaluate the effect of the traditional Chinese medicine ( Jingui Shenqi Wan ) combined with excellent environment emotion regulation on the growth retardation , abnormal behavior and serum neurotransmitters in the pregnant rats with post - traumatic stress after trauma .
The growth and development indexes such as body weight , body length , tail length ( 0 , 5 , 15 , 25 d ) and ear ( day 5 ) of each group were measured repeatedly .
The levels of serum neurotransmitters such as cortical ketone , 5 - HT and dopamine were measured by enzyme - linked immunosorbent assay . The results showed that the body weight , body length and tail length were lower than those in the control group and the case group ( P0.05 ) .
Body weight , body length and tail length were statistically significant ( P < 0.05 ) . Body weight , body length , tail length were affected by time factors , body weight , body length and tail length were statistically significant ( P < 0.05 ) .
The time factor and the interaction of grouping factors had statistical significance ( P < 0.05 ) , that is , the trend of body weight , body length and tail length changed from time to time .
There was significant difference ( P 0.05 ) 2 . In the test of behavioral ability test , the ability to turn over was lower than that in the control group and the case group ( P0.01 ) .
Compared with the control group , the difference was not statistically significant ( P0.05 ) .
Compared with the control group , the difference of stool number was significant ( P0.05 ) . There was no statistical significance ( P0.05 ) . The time difference was statistically significant ( P0.05 ) .
Compared with the control group , there was no statistical significance ( P0.05 ) . In the elevated cross maze test , the percentage of open arms and the percentage of staying time of open arms were lower than those in the control group and the control group ( P0.05 ) .
The percentage of open arms and the percentage of staying time of open arms were higher than those in the control group ( P0.05 ) .
Compared with the control group , the difference was not significant ( P0.05 ) . The levels of serum 5 - HT were lower than those in the control group and the control group ( P0.05 ) .
The levels of 5 - HT in the patients were higher than those in the control group ( P0.05 ) .
The results showed that the harm of the stress caused by traumatic stress in the pregnant period of the female rats could be inherited to the progeny , which led to the low birth weight ( long tail length ) , the growth and growth retardation , the behavior and the abnormal serum neurotransmitter .

【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R248

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