咖啡因对人体疼痛模型针刺镇痛效应的影响

发布时间：2018-04-20 21:34

本文选题：针刺 + 疼痛　；参考：《成都中医药大学》2016年硕士论文

【摘要】：目的：明确咖啡因对人体疼痛模型针刺镇痛效应以及腺苷含量的影响。方法：将符合纳入标准的受试者进行随机分组,分为0 mg咖啡因组、200 mg咖啡因组、400 mg咖啡因组；人体疼痛模型的建立采用钾离子透入法；干预方法为给予不同剂量咖啡(分别含有0 mg、200 mg、400 mg咖啡因)摄入和针刺左侧足三里穴位;于左侧足三里穴、右侧足三里穴、左侧合谷穴处记录基线、喝咖啡30分钟后、针刺10分钟、20分钟、30分钟时、休息30分钟后的痛阈；在基线、喝咖啡30分钟后、针刺30分钟后、针刺结束30分钟后,共4个时间点收集血浆样品,用]HPLC检测血浆中咖啡因和腺苷的含量；统计分析采用SPSS21.0统计软件处理；本研究试验前已在Clinical Trials.gov获得注册,注册号：NCT02577770。结果：1.咖啡因对针刺镇痛效应的影响：1)左侧足三里穴位处痛阈变化：0 mg咖啡因组在针刺期间痛阈明显上升,在针刺10分钟、20分钟、30分钟时的痛阈分别上升65%、79%、97%,随时间增加,痛阈不断升高,针刺结束休息30分钟后回降至基线水平,与200 mg咖啡因组和400 mg咖啡因组比较,差异均有统计学意义(P0.05)；200 mg咖啡因组和400 mg咖啡因组的痛阈一直处于降低趋势,且两组比较,差异无统计学意义(P0.05)。2)右侧足三里穴位处痛阈变化：0 mg咖啡因组在针刺期间痛阈明显上升,在针刺10分钟、20分钟、30分钟时的痛阈分别上升74%、73%、73%,至针刺结束休息30分钟后回降,但仍未恢复至基线水平,与200 mg咖啡因组和400 mg咖啡因组比较,差异均有统计学意义(P0.05)；200 mg咖啡因组和400 mg咖啡因组的痛阈一直处于降低趋势,且两组比较,差异无统计学意义(P0.05)。3)左侧合谷穴位处痛阈变化：0 mg咖啡因组在针刺期间痛阂明显上升,在针刺10分钟、20分钟、30分钟时的痛阈分别上升41%、44%、51%,且随时间增加,痛阈不断升高,至休息30分钟后回降至基线水平,与200 mg咖啡因组和400 mg咖啡因组比较,差异均有统计学意义(P0.05)；200 mg咖啡因组和400mg咖啡因组的痛阈一直处于降低趋势,且两组比较,差异无统计学意义(P0.05)。2.血浆中咖啡因含量变化：0 mg咖啡因组在基线、喝咖啡30分钟后、针刺30分钟后、休息30分钟后,血浆中均未测到咖啡因；200 mg咖啡因组在基线、喝咖啡30分钟后、针刺30分钟后、休息30分钟后,血浆中咖啡因含量分别为：0 μg/mL、4.48 μg/mL、5.89 μg/mL、6.10μg/mL; 400 mg咖啡因组在基线、喝咖啡30分钟后、针刺30分钟后、休息30分钟后,血浆中咖啡因含量分别为：0 μg/mL、10.29μg/mL、14.18μg/mL、 13.49 μg/mL3.血浆中腺苷含量变化：0 mg咖啡因组在针刺期间腺苷含量明显上升52%,与200 mg咖啡因组和与400 mg咖啡因组比较,差异有统计学意义(P0.05),至针刺结束休息30分钟后回降,但仍高于基线水平,与400 mg咖啡因组比较,差异有统计学意义(P0.05)；200 mg咖啡因组腺苷含量在喝咖啡30分钟后下降11%,针刺30分钟后上升6%,至针刺结束休息30分钟后回降至基线,400 mg咖啡因组腺苷含量呈逐渐下降趋势,两组比较,差异均无统计学意义(P0.05)。结论：1.针刺治疗期间摄入咖啡因,可抑制针刺镇痛效应；2.200 mg咖啡因和400 mg咖啡因抑制针刺镇痛效应的程度相同；3.血浆中的腺苷可能参与针刺镇痛,而咖啡因摄入后,可能会影响针刺镇痛期间血浆中的腺昔含量。
[Abstract]:Objective: to determine the effect of caffeine on the analgesic effect and adenosine content of acupuncture in human pain model. Methods: the subjects were randomly divided into 0 mg caffeine groups, 200 mg caffeine group and 400 mg caffeine group, and the body pain model was established by potassium ion penetration method; the intervention method was given to different agents. Intake of coffee (0 mg, 200 mg, 400 mg caffeine respectively) intake and acupuncture point on the left Zusanli; at the left Zusanli point, the right Zusanli point, the left Hegu Point at the baseline, 30 minutes after drinking coffee, 10 minutes, 20 minutes, 30 minutes, 30 minutes after the pain threshold; after the coffee 30 minutes after coffee 30 minutes, acupuncture 30 minutes after 30 minutes, acupuncture, acupuncture 30 minutes after the needle, needles 30 minutes after the needle, needles 30 minutes after 30 minutes, needles after 30 minutes, needles 30 minutes after acupuncture needles, needles 30 minutes after the needle, needles 30 minutes after acupuncture, needles 30 minutes after the needle, needles 30 minutes after acupuncture, needles 30 minutes after the needle, needles 30 minutes after acupuncture, needles 30 minutes after the needle, needles 30 minutes after acupuncture, needles 30 minutes after acupuncture, needles 30 minutes after acupuncture needles, needneedles 30 minutes after 30 minutes, needles, needles 30 minutes after the stings, the plasma samples were collected at 4 time points, and the content of caffeine and adenosine in the plasma was detected by]HPLC; the statistical analysis was processed by the SPSS21.0 statistical software; the study was registered before Clinical Trials.gov, and the registration number: NCT02577770. results: the effect of 1. caffeine on the analgesic effect of acupuncture: 1) left side The pain threshold at Zusanli point: the pain threshold of 0 mg caffeine group increased obviously during the acupuncture period. The pain threshold increased by 65%, 79%, 97% at 10 minutes, 20 minutes and 30 minutes respectively. The pain threshold increased with time, and the end of acupuncture ended for 30 minutes to the baseline level, and the difference was compared with the 200 mg caffeine group and 400 mg caffeine group. There was statistical significance (P0.05); the pain threshold of 200 mg caffeine group and 400 mg caffeine group had been decreasing, and the two groups had no statistically significant difference (P0.05).2) the pain threshold at the right Zusanli point: the pain threshold of the 0 mg caffeine group increased significantly during the acupuncture period, and the pain threshold increased at 10 minutes, 20 minutes and 30 minutes respectively. 74%, 73%, 73%, until the end of the acupuncture rest 30 minutes after the fall, but still not to the baseline level, compared with the 200 mg caffeine group and 400 mg caffeine group, the difference was statistically significant (P0.05); 200 mg caffeine group and 400 mg caffeine group of the pain threshold has been in the lower trend, and the two groups, the difference is not statistically significant (P0.05).3) left The pain threshold changes at Hegu acupoint: 0 mg caffeine group in the period of acupuncture during acupuncture significantly increased the pain, the pain threshold increased by 41%, 44%, 51% at 30 minutes, 20 minutes and 30 minutes, and the pain threshold increased with time. After 30 minutes of rest, the pain threshold was reduced to the baseline level. Compared with 200 mg caffeine group and 400 mg caffeine group, there were statistical differences. Learning significance (P0.05); 200 mg caffeine group and 400mg caffeine group's pain threshold has been in a decreasing trend, and the two groups, the difference is not statistically significant (P0.05).2. plasma caffeine content changes: 0 mg caffeine group in the baseline, 30 minutes after drinking coffee, 30 minutes after acupuncture, after 30 minutes of rest, the plasma is not caffeine, 200 mg caffeine. After 30 minutes of coffee in the base line of enkephalin group, after 30 minutes of acupuncture, after 30 minutes of rest, the caffeine content in plasma was 0 mu g/mL, 4.48 mu g/mL, 5.89 g/mL, 6.10 g/mL; 400 mg caffeine group was in the baseline, after coffee for 30 minutes, after 30 minutes of acupuncture, the caffeine content in plasma was: 0, g/mL, 10.29 g/mL, respectively. The changes of adenosine content in 14.18 g/mL and 13.49 g/mL3. plasma: 0 mg caffeine group increased by 52% during acupuncture, and compared with 200 mg caffeine group and 400 mg caffeine group, the difference was statistically significant (P0.05), until the end of acupuncture at the end of rest for 30 minutes, but still higher than the baseline level, compared with the 400 mg caffeine group, the difference was compared. Statistical significance (P0.05); 200 mg caffeine group adenosine content decreased 11% after 30 minutes of coffee, 30 minutes after acupuncture, up 6%, to the end of the needle rest 30 minutes back to the baseline, 400 mg caffeine group adenosine content decreased gradually, two groups, the difference was not statistically significant (P0.05). Conclusion: 1. during the acupuncture treatment period photography. Caffeine can inhibit the analgesic effect of acupuncture; 2.200 mg caffeine and 400 mg caffeine inhibit the same degree of acupuncture analgesia; 3. the adenosine in plasma may be involved in acupuncture analgesia, and caffeine intake may affect the adenosine content in the plasma during acupuncture analgesia.

【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R246

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