电针百会、大椎、肾俞穴对SAMP8小鼠学习记忆与海马CA1区神经元突触的影响

发布时间：2018-04-24 14:58

本文选题：阿尔茨海默病 + 电针　；参考：《中国康复理论与实践》2017年11期

【摘要】：目的观察电针百会、大椎、肾俞穴对SAMP8小鼠学习记忆与海马CA1区神经元突触的影响,探讨电针治疗阿尔茨海默病(AD)的机制。方法 7月龄SAMP8小鼠24只随机分为模型组和电针组,同龄正常老化SAMR1小鼠12只为对照组。电针组电针百会、大椎、肾俞穴30 d。Morris水迷宫检测小鼠学习记忆能力,免疫组织化学观察小鼠海马CA1区突触素(SYN)及突触后致密区蛋白95(PSD95)的表达,透射电镜观察小鼠海马CA1区突触超微结构的变化。结果与模型组相比,电针组逃避潜伏期缩短(P0.05),原平台象限停留时间延长(P0.05),穿越原平台次数增加(P0.05),海马CA1区SYN与PSD95表达显著提高(P0.001),突触结构较完整,数量增加。结论电针能提高小鼠海马CA1区神经元突触蛋白的表达,改善突触的超微结构,从而有效改善SAMP8小鼠的学习记忆能力。
[Abstract]:Objective to observe the effects of electroacupuncture at Baihui, Dazhui and Shenshu on learning and memory and synapses of hippocampal CA1 neurons in SAMP8 mice, and to explore the mechanism of electroacupuncture in treating Alzheimer's disease (AD). Methods 24 SAMP8 mice aged 7 months were randomly divided into two groups: model group and electroacupuncture group. 12 normal aged SAMR1 mice of the same age were used as control group. In the electroacupuncture group, the ability of learning and memory was detected by water maze at Baihui, Dazhui and Shenshu points for 30 d.Morris, and the expression of synaptophysin (SYN) and PSD95 (PSD95) in hippocampal CA1 region were observed by immunohistochemistry. Ultrastructural changes of synapses in CA1 region of hippocampus of mice were observed by transmission electron microscope. Results compared with the model group, the escape latency of electroacupuncture group was shortened (P 0.05), the residence time of the original platform quadrant was prolonged (P 0.05), the times of crossing the original platform was increased (P 0.05), the expression of SYN and PSD95 in hippocampal CA1 region was significantly increased (P 0.001), the synaptic structure was relatively complete and the number of synapses was increased. Conclusion electroacupuncture can improve the expression of synaptic protein and the ultrastructure of synapses in CA1 neurons of mice, and thus improve the ability of learning and memory in SAMP8 mice.
【作者单位】：福建中医药大学针灸学院;甘肃省监狱管理局兰州医院;福建中医药大学康复学院;福建中医药大学中西医结合学院;
【基金】：国家自然科学基金项目(No.81102625) 福建省自然科学基金项目(No.2017J01541) 福建省中医药科研项目(No.2017FJZYJC102) 2015年度福建省高等学校新世纪优秀人才支持计划项目
【分类号】：R245

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