养血祛风方对抽动障碍鼠行为及PSD-95表达水平的影响

发布时间：2018-04-25 05:27

本文选题：抽动障碍 + 养血祛风方　；参考：《北京中医药大学》2016年硕士论文

【摘要】：抽动障碍是一种起病于儿童时期、复杂的、慢性神经精神障碍,以多种运动和发声抽动为主要临床表现,其患病率男性大于女性,常伴有强迫、多动等行为和情绪障碍,研究表明,与前额叶-纹状体相关的认知损害可能和抽动障碍的临床症状相关,认知损害以及和注意力缺陷多动障碍、强迫症等较高的共病率严重影响儿童的学业、同伴关系、社会适应能力以及生活质量,妨碍儿童个性的形成及心理品质的健康发展。本研究就养血祛风方对抽动障碍模型鼠抽动行为、认知能力及脑组织纹状体PSD-95表达的影响进行研究,探讨养血祛风方治疗儿童抽动障碍的疗效及可能的作用机制,为养血祛风方治疗儿童抽动障碍的临床应用提供实验依据。论文主要包括文献综述及实验研究两部分。文献综述分为“现代医学对抽动障碍的研究概况”和“中医学对抽动障碍的研究概况”两部分。通过查阅近年来国内外研究儿童抽动障碍的相关文献资料,就儿童抽动障碍现代医学在流行病学、发病机制、诊断标准、治疗、动物模型、PSD-95以及中医在病因病机、治法方药等方面进行了较为系统的总结。实验研究目的：通过建立抽动障碍大鼠模型,观察其行为学改变以及脑组织纹状体PSD-95水平的变化,对动物模型的复制进行评价,探讨养血祛风方治疗抽动障碍的作用机制,并与西药泰必利进行药效学比较,为临床中医药的开发应用奠定实验基础。探索养血祛风方在治疗多发性抽动障碍,尤其在运动性抽动及学习记忆方面的作用,为临床治疗抽动障碍从血论治提供依据。方法：IDPN腹腔注射建立大鼠拟TD模型,将TD大鼠随机分为正常组、模型组、西药组及中药低剂量组、中药中剂量组、中药高剂量组,造模后分别于第1、4、8周对各组大鼠进行行为学观察,并进行旷场分析及水迷宫测试,评价大鼠学习和记忆能力。采用HE染色法观察大鼠神经元锥体、颗粒细胞形态结构,并采用免疫组化方法检测大鼠纹状体PSD-95表达水平的变化。结果：与正常组比较,模型组跨格次数增加,垂直活动次数明显减少；与模型组比较,中药中、高剂量组及西药组中央停格时间有统计学意义(P0.05),中药低、高剂量组跨格次数有统计学意义(P0.05),中药中、高剂量组垂直活动次数有统计学意义(P0.05);第4、8周中药高剂量组大鼠半数能完成水迷宫测试,与模型组比较,差异有统计学意义(P0.05)。HE染色结果：TS模型大鼠纹状体神经元细胞形态结构发生病理性改变；通过药物干预可使其改善,中药中剂量组及中药高剂量组优于中药低剂量组及西药组。PSD-95免疫组化结果：模型组大鼠纹状体组织切片PSD-95受体表达水平与其他5组相比明显减少。经治疗在PSD-95层面中药高剂量组与中药中剂量组无明显差异,但均优于中药低剂量组及西药组。结论：养血祛风方中、高剂量组有改善抽动障碍大鼠空间学习记忆能力的作用,且大鼠纹状体中PSD-95受体的减少与抽动症的发生存在相关性。
[Abstract]:Disorder is a kind of childhood, complicated, chronic neuromental disorder, with multiple exercise and sound twitch as the main clinical manifestation. The prevalence rate of male is greater than that of women, often accompanied by compulsion, hyperactivity and emotional disorders. Research shows that cognitive impairment associated with prefrontal lobes may be associated with the clinical symptoms of twitches. The higher rates of common disease such as cognitive impairment and attention deficit hyperactivity disorder, obsessive-compulsive disorder, and obsessive-compulsive disorder seriously affect children's study, peer relationship, social adaptation and quality of life, which impede the formation of children's personality and the healthy development of psychological quality. The effect of force and the expression of PSD-95 in the striatum of the brain tissue is studied. The effect and possible mechanism of the prescription of nourishing blood and dispelling wind in the treatment of children's aspiration disorders are discussed. The paper provides the experimental basis for the clinical application of blood and dispelling wind prescription in the treatment of children's aspiration disorders. The paper mainly includes two parts: literature review and laboratory study. The literature review is divided into "modern medicine" The two part of the study on the disorders of aspiration "and" the general situation of the study of Chinese medicine to the disorders of aspiration ". By consulting the relevant literature on children's aspiration disorders at home and abroad in recent years, the epidemiology, pathogenesis, diagnostic criteria, treatment, animal models, PSD-95 and TCM in the etiology and pathogenesis of children with twitch disorders are reviewed. The objective of this study was to establish a rat model of motor disorder, to observe the changes of the behavioral changes and the changes in the PSD-95 level of the striatum in the brain tissue, to evaluate the reproduction of the animal model and to explore the mechanism of the treatment of withdrawal disorders by the prescription of nourishing blood and dispelling wind, and to carry out with the Western drug taapride. Pharmacodynamic comparison, in order to establish the experimental basis for the development and application of clinical traditional Chinese medicine. Explore the role of nourishing blood and dispelling wind in the treatment of multiple aspiration disorders, especially in the aspects of motor movement and learning and memory, and provide a basis for clinical treatment of aspiration disorders from blood. Method: IDPN intraperitoneal injection of pseudo TD model of rats was established, and TD rats were randomly divided into two groups. The normal group, the model group, the western medicine group and the low dose group of traditional Chinese medicine, the middle dose group of traditional Chinese medicine and the high dose group of traditional Chinese medicine were observed on the behavior of rats in week 1,4,8, and the field analysis and water maze test were carried out to evaluate the learning and memory ability of the rats. The HE staining method was used to observe the neuron pyramids and the morphologic structure of the granulosa cells. The change of PSD-95 expression level in the striatum of rats was detected by immunohistochemical method. Results: compared with the normal group, the number of cross lattice increased and the number of vertical activity decreased obviously. Compared with the model group, the central stopping time of the high dose group and the western medicine group was statistically significant (P0.05), the traditional Chinese medicine was low, and the high dose group had the cross grid times. Statistical significance (P0.05), the number of vertical activities in high dose group was statistically significant (P0.05), and half of the rats in the high dose group could complete the water maze test at week 4,8, and compared with the model group, the difference was statistically significant (P0.05).HE staining results: the morphological structure of the neurons in the striatum of the TS model rats had a pathological change; The dosage group and the high dose group of traditional Chinese medicine were superior to the low dose group and the western medicine group.PSD-95 immunohistochemical results: the expression level of PSD-95 receptor in the striatum tissue section of the model group was significantly reduced compared with the other 5 groups. The high dose group and the middle dose group of the Chinese medicine were not obvious at the PSD-95 level. The difference is better than the low dose group of Chinese medicine and the western medicine group. Conclusion: the high dose group has the effect of improving the spatial learning and memory ability of the rats with aspiration, and the decrease of PSD-95 receptor in the striatum of rats is related to the occurrence of aspiration.

【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R277.7

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