大蒜硝黄膏对SD大鼠粘连性肠梗阻的治疗作用及对皮肤性能的影响

发布时间：2018-04-29 14:09

本文选题：大蒜硝黄膏 + 穴位贴敷　；参考：《河北大学》2016年硕士论文

【摘要】：目的:考察大蒜硝黄膏对SD大鼠粘连性肠梗阻的治疗作用,对皮肤结构和力学性能的影响,探讨其透皮吸收机理,为大蒜硝黄膏治疗粘连性肠梗阻的临床应用提供参考。方法:1.将32只大鼠随机分成4组(n=8),即空白组、模型组、阳性对照组、实验组,除空白组外均采用浆膜剥脱法建立粘连性肠梗阻模型,造模期间第五、七天拍腹部平位片观察造模情况,造模成功后连续给药五天,给药结束后处死大鼠观察大蒜硝黄膏对大鼠一般身体状态、小肠粘连程度、小肠形态的影响。2.将大蒜硝黄膏贴敷于大鼠腹部,观察不同时间点(1h、2h、3h、4h、6h)对皮肤结构的影响。3.取大鼠、小鼠皮肤,在不同赋合剂(姜汁组、醋组、植物油组、空白组)中分别浸泡6h,测量皮肤拉伸强度。结果:1.大蒜硝黄膏对SD大鼠粘连性肠梗阻的治疗作用实验结果表明:模型组大鼠一般情况差,小肠黏膜损伤严重,粘连分级高。实验组和阳性对照组均能不同程度的改善大鼠的一般状况,降低小肠黏膜损伤和粘连程度。2.大蒜硝黄膏贴敷1h时,大鼠活性表皮和角质层变化不明显,贴敷2h时,角质层开始松散,贴药3h、4h时皮肤表皮、角质层松散,贴药6h时皮肤活性表皮回复正常。3.不同的粘合赋形剂均能降低大鼠和小鼠皮肤的拉伸强度,白醋作用最强,拉伸强度降低程度由大到小顺序为:白醋植物油姜汁。结论:1.大蒜硝黄膏对浆膜剥脱法粘连性肠梗阻模型大鼠有一定的治疗作用。2.贴敷大蒜硝黄膏使皮肤角质层疏松脱落,皮肤细胞间隙增加,有利于药物透皮吸收。3.穴位贴敷的赋性剂醋、姜汁、植物油可使皮肤结构变得疏松,细胞间结合能力变弱,其抗拉强度减弱,有利于药物透皮吸收。
[Abstract]:Objective: to investigate the therapeutic effect of garlic Nihuang ointment on adhesive intestinal obstruction in SD rats and its effects on skin structure and mechanical properties, and to explore the mechanism of its transdermal absorption, and to provide reference for the clinical application of garlic Nihuang ointment in the treatment of adhesive intestinal obstruction. Method 1: 1. Thirty-two rats were randomly divided into 4 groups: blank group, model group, positive control group and experimental group. The adhesive intestinal obstruction model was established by serous stripping method except for blank group. The rats were killed at the end of the administration to observe the effects of garlic nitrate ointment on the general state of the body, the degree of adhesion of the small intestine, and the morphology of the small intestine. The effects of garlic nitrate ointment on the skin structure of rats were observed at different time points (1 h, 2 h, 3 h, 4 h, 6 h). The skin of rats and mice were soaked in different Fu mixture (ginger juice group, vinegar group, vegetable oil group, blank group) for 6 hours, and the skin tensile strength was measured. The result is 1: 1. The therapeutic effect of garlic niihuang ointment on adhesive intestinal obstruction in SD rats was studied. The results showed that the rats in the model group were in poor condition, the intestinal mucosa was damaged seriously, and the adhesion grade was high. Both the experimental group and the positive control group could improve the general condition of the rats in varying degrees and reduce the intestinal mucosal injury and adhesion. The changes of active epidermis and cuticle of rats were not obvious at 1h after application of garlic Nihuang ointment. At 2h, the cuticle began to loose, the skin epidermis and cuticular layer loosened at 3h or 4h, and the active epidermis returned to normal at 6h. Different adhesive excipients could decrease the tensile strength of the skin of rats and mice. The effect of white vinegar was the strongest, and the degree of decrease of tensile strength was in the order of white vinegar vegetable oil ginger juice. Conclusion 1. Garlic Nihuang ointment has a certain therapeutic effect on adherent intestinal obstruction model rats. 2. 2. The application of garlic Nihuang ointment made the cuticle loose and the gap of skin cells increased, which was beneficial to drug transdermal absorption. 3. Vinegar, ginger juice and vegetable oil applied at acupoint can make skin structure loose, intercellular binding ability weaken, and its tensile strength weaken, which is beneficial to drug transdermal absorption.
【学位授予单位】：河北大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

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