穴位贴敷对过敏性哮喘大鼠血清转化生长因子β1和尾加压素Ⅱ的影响

发布时间：2018-04-30 04:31

  本文选题：过敏性哮喘 + 穴位贴敷　； 参考：《中医杂志》2017年22期

【摘要】：目的探讨穴位贴敷治疗过敏性哮喘的可能作用机制。方法将40只Wistar大鼠随机分为空白组、模型组、贴敷2 h组、贴敷4 h组,每组10只。除空白组外,其余各组大鼠用鸡卵白蛋白(OVA)和生理盐水的混悬液皮下多点注射及雾化吸入致敏制造过敏性哮喘模型。造模结束后第15天起,空白组和模型组正常饲养;贴敷2 h组、贴敷4 h组于大鼠肺俞、脾俞、肾俞穴位贴敷,分别贴敷2 h、4 h,隔天1次,共贴7次。观察各组大鼠引喘潜伏期和发作总持续时间;ELISA法检测大鼠血清中转化生长因子β1(TGF-β1)和尾加压素Ⅱ(UⅡ)含量;HE染色观察大鼠支气管和肺组织病理结构改变。结果与模型组比较,贴敷2 h、4 h组引喘潜伏期延长、发作总持续时间缩短,血清TGF-β1和UⅡ水平明显降低(P0.01);支气管和肺组织结构不清,支气管黏膜水肿、充血及气道壁增厚等改变显著改善。贴敷4 h组与贴敷2 h组相比,引喘潜伏期、发作总持续时间、血清TGF-β1与UⅡ差异均无统计学意义(P0.05)。结论穴位贴敷能降低血清TGF-β1和UⅡ含量,从而改善气道重塑,可能是其治疗过敏性哮喘的机制之一。
[Abstract]:Objective to explore the possible mechanism of acupoint application in the treatment of allergic asthma. Methods Forty Wistar rats were randomly divided into blank group, model group, 2 h group and 4 h group with 10 rats in each group. The allergic asthma model was induced by subcutaneous injection of ovalbumin (OVA) and normal saline suspension and atomization inhalation in the other groups except the blank group. From the 15th day after the model was finished, the blank group and the model group were fed normally, while the group of 2 h and the group of 4 h were applied at the acupoints of Fesu, Pi Yu and Shenshu, respectively, for 2 hours and 4 hours respectively, and once every other day for a total of 7 times. The latent period of induced asthma and the total duration of attack were detected by Elisa. The contents of TGF- 尾 1 and U2) in serum of rats were detected. The pathological changes of bronchi and lung were observed by HE staining. Results compared with the model group, the incubation period of induced asthma was prolonged, the duration of attack was shortened, the serum levels of TGF- 尾 1 and U 鈪，

本文编号：1823109